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Immunocytochemical localization of trehalase inhibitor in some insect species

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Abstract

Antibody against cockroach trehalase inhibitor was prepared and tested against the plasma of adult locusts and larval silkworms to determine whether these species possess a similar protein. An immunopositive response was elicited in both species. Studies using immunogold labeling show that adult cockroaches have trehalase inhibitor protein in granules of plasmatocytes and in oenocytoid-like structures. Localization of the immunoreactive protein with trehalase inhibitor antibody in locust hemocytes indicated that the protein is also contained in the granules of plasmatocytes. However, in the hemolymph of silkworm larvae, the immunoreactive protein was found only in the spherules of spherulocytes. The results suggest that insect hemolymph commonly contains trehalase inhibitor both in plasma and in certain hemocytes.

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Authors and Affiliations

  1. Department of Medical Entomology, National Institute of Health, 1-23-1, Toyama, Shinjuku-ku, 162, Tokyo, Japan

    T. Hiraoka

  2. Biochemical Laboratory, Institute of Low Temperature Science, Hokkaido University, 060, Sapporo, Japan

    Y. Hayakawa

  3. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    R. G. H. Downer

Authors
  1. T. Hiraoka
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  2. Y. Hayakawa
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  3. R. G. H. Downer
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Hiraoka, T., Hayakawa, Y. & Downer, R.G.H. Immunocytochemical localization of trehalase inhibitor in some insect species. Cell Tissue Res 279, 465–468 (1995). https://doi.org/10.1007/BF00318159

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  • DOI: https://doi.org/10.1007/BF00318159

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