Abstract.
Blastemal cells of embryonic mouse limb buds (day 12) were cultivated in organoid cultures in the presence of the human recombinant cytokines interleukin-1β (IL-1β) and tumour necrosis factor α (TNF-α). The effects of both cytokines (applied alone or together) on mesenchymal cells were demonstrated by electron microscopy. Cultures treated with TNF-α (alone or in combination with IL-1β) showed several mesenchymal cells with numerous irregularly shaped membrane-bordered cavities containing thick bundled tannic-acid-positive fibrillar structures that resembled loosened collagen fibrils, whereas cells exposed to IL-1β alone did not exhibit such changes. These findings are discussed in the light of two hypotheses: the phagocytosis of extracellular collagen fibrils, and fibrillogenesis resulting from incongruity of synthesis and secretion rates of procollagen; our results favour the former.
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Received: 14 March 1995 / Accepted: 7 February 1996
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Mohamed-Ali, H., Scholz, P. & Merker, HJ. Intracellular collagen-like material in mouse embryonic mesenchymal cells incubated with TNF-α and IL-1β in organoid culture. Cell Tissue Res 284, 509–515 (1996). https://doi.org/10.1007/s004410050613
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DOI: https://doi.org/10.1007/s004410050613