Summary
Measurement of the B-interphase has afforded a means of calculating the duration of the cell division cycle at various temperatures, from 0° to 40° C, in the root meristems of Allium cepa. For this purpose caffeine labelling was used to produce a small population (1–5%) of highly synchronized binucleate cells. The B-interphase is what we call the period from 0 hour (end of the one-hour caffeine treatment) to the appearance of the first binucleate cells in bi-prophase. The duration of the phases that make up the cycle was calculated from their relative frequency within the meristematic population.
Observation shows that at 0° and at 40° the cells do not go through the cycle at all and that from 10° to 30° the speed increases in almost linear proportion. There is no significant change in the relative duration of any of the morphological phases under any of the conditions tested.
In discussing these results the authors propose the use of the cell flow (percentage of meristematic cells passing through any point in the cycle in one hour) as an expression in the simplest form of the steady-state kinetics of the meristematic population at a given temperature.
Similar content being viewed by others
References
Agrell, I.: The thermal dependence of the mitotic stages during the early embryonic development of the sea urchin embryo. Arch. Zool. (Stockh.) 11, 383–392 (1958).
Bajer, A., and J. Mole-Bajer: Endosperm; material for study on the physiology of cell division. Acta. Soc. Bot. Pol. 23, 69–110 (1954).
Brown, R. J.: The effects of temperature on the durations of the different stages of cell division in the root-tip. Exp. Bot. 2, 96–110 (1951).
Edwards, J. L., A. L. Koch, P. Youcis, H. L. Freese, M. B. Laite, and J. T. Donalson: Some characteristics of DNA synthesis and the mitotic cycle in Ehrlich ascites tumor cells. J. biophys. biochem. Cytol. 7, 273–282 (1960).
Ephrussi, B.: Sur les coefficients de température des différentes phases de la mitose des oeufs d'oursin (Paracentrotus lividus Lk.) et de l'ascaris megalocephala. Protoplasma (Wien) 1, 105–123 (1927).
Evans, H. J., and J. R. K. Savage: The effect of temperature on mitosis and on the action of colchicine in root meristem cells of Vicia faba. Exp. Cell Res. 18, 51–61 (1959).
Giménez-Martin, G., A. González-Fernández, and J. F. López-Sáez: A new method of labelling cells. J. Cell Biol. 26, 305–309 (1965).
- - - Duration of the division cycle in mononucleate, binucleate and tetraploid cells. Exp. Cell Res. (in press).
González-Fernández, A., J. F. López-Sáez, and G. Giménez-Martin: Inhibition of cytokinesis: bimitosis and polymitosis. Fiton 21, 157–165 (1964).
- - - Duration of the division cycle in mononucleate and binucleate cells. Exp. Cell Res. (in press).
Hejnowicz, Z.: Growth and cell division in the apical meristem of wheat roots. Physiol. Plantarum (Kbh.) 12, 124–138 (1959).
Hoffman, J. G.: Theory of the mitotic index and its application to tissue growth measurement Bull. Math. Biophysics 11, 139–144 (1949).
Hughes, A.: The mitotic cycle, p. 89. London: Butterworths Sci. Publ. 1952.
Kihlman, B.: Chromosome breakage in allium by 8-ethoxycaffeine and X-rays. Exp. Cell Res. 8, 345–368 (1955).
López-Sáez, J. F., and E. Fernández-Gómez: Mitotic partial index and phase indices. Experientia (Basel) 21, 591–592 (1965).
—, M. C. Risueño, and G. Giménez-Martin: Inhibition of cytokinesis in plant cells. J. Ultrastruct. Res. 14, 85–92 (1966).
Tjio, J. H., and A. Levan: The use of oxiquinoline in chromosome analysis. Ann. Estac. Exptl. Aula Dei 2, 21–64 (1950).
Van't Hoff, J., and A. H. Sparrow: The effect of mitotic cycle duration on chromosome breakage in meristematic cells of Pisum sativum. Proc. nat. Acad. Sci. 50, 855–860 (1963).
—, G. B. Wilson, and A. Colon: Studies on the control of mitotic activity: the use colchicine in the tagging of a synchronous population of cells in the meristem of Pisum sativum. Chromosoma (Berl.) 11, 313–321 (1960).
—, and W. K. Ying: Relationship between the duration of the mitotic cycle, the rate of cell production and the rate of growth of Pisum roots at different temperatures. Cytologia (Tokyo) 29, 399–406 (1964).
Wimber, D.: Duration of the nuclear cycle in Tradescantia paludosa root tips as measured with H3-thymidine. Amer. J. Bot. 47, 828–834 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
López-Sáez, J.F., Giménez-Martin, G. & Gonzalez-Fernández, A. Duration of the cell division cycle and its dependence on temperature. Zeitschrift für Zellforschung 75, 591–600 (1966). https://doi.org/10.1007/BF00341516
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00341516