Skip to main content
Log in

Differentiation of proteinurias with electrophoresis

  • Original Article
  • Editorial Comment
  • Published:
Pediatric Nephrology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Lütscher JA (1940) Electrophoretic analysis of plasma and urine proteins. J Clin Invest 19:313–320

    Google Scholar 

  2. Longsworth LG, MacInnes DA (1940) An electrophoretic study of nephrotic sera and urine. J Exp Med 71:77–82

    Google Scholar 

  3. Davies BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427

    PubMed  Google Scholar 

  4. Ornstein L (1964) Disc electrophoresis. I. Background and theory. Ann NY Acad Sci 121:321–349

    PubMed  Google Scholar 

  5. Raymond S, Weintraub L (1959) Acrylamide gel as a supporting medium for zone electrophoresis. Science 130:711–717

    Google Scholar 

  6. Maurer HR (1968) Disk Elektrophorese. De Gruyter, Berlin

    Google Scholar 

  7. Neuhoff V (1970) Die Disk-Elektrophorese als analytische Mikromethode zur Charakterisierung von Makromolekülen. Mitt Dtsch Pharm Ges 12:289–314

    Google Scholar 

  8. Weise M, Bockhorn H (1972) Discontinuous micro-disc electrophoresis for quantitative albumin determination in urine of patients with kidney transplants. Clin chim Acta 42:391–395

    Google Scholar 

  9. Shapiro AL, Vinuela E, Maizel JV (1967) Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun 28:815–821

    PubMed  Google Scholar 

  10. Weber K, Osborn M (1969) The reliability of molecular weight determination by dodecyl-sulfate-polyacrylamide gel electrophoresis. Biol Chem 244:4406–4412

    PubMed  Google Scholar 

  11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680–685

    PubMed  Google Scholar 

  12. Pesce AJ, Boreisha I, Pollak VE (1972) Rapid differentiation of glomerular and tubular proteinuria by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Clin Chim Acta 40:27–34

    PubMed  Google Scholar 

  13. Boesken WH, Kopf K, Schollmeyer P (1973) Differentiation of proteinuric diseases by discelectrophoretic molecular weight analysis of urinary proteins. Clin Nephrol 4:311–318

    Google Scholar 

  14. Maurer HR, Allen AC (1972) Polyacrylamide gel electrophoresis in clinical chemistry: problems of standardization. Clin Chim Acta 40: 359–370

    PubMed  Google Scholar 

  15. Rüchel R, Mesecke S, Wolfrum DD, Neuhof V (1974) Microelectrophoresis in continuous polyacrylamide gels. II. Fractionation and dissociation of sodium dodecyl sulfate protein complexes. Hoppe Seyler Z Physiol Chem 355:997–1020

    Google Scholar 

  16. Reichel W, Wolfrum DI, Klein R, Scheler F (1976) Differenzierung der Proteinurie durch die Mikroelektrophorese in kontinuierlichen Polyacrylamid-Gradientengelen. Klin Wochenschr 54:19–24

    PubMed  Google Scholar 

  17. Alt JM, Deerberg F, Hackbarth H, Stolte H (1980) The study of urinary protein excretion in male rats as compared with human proteinuria. Contrib Nephrol 19:79–87

    PubMed  Google Scholar 

  18. Rüchel R, Mesecke S, Wolfrum DI, Neuhoff V (1973) Mikroelektrophorese an kontinuierlichen Polyacrylamid-Gradienten. I. Herstellung und Eigenschaften von Gelgradienten in Kapillaren, ihre Anwendung zur Proteinfraktionierung und Molekulargewichtsbestimmung. Hoppe Seyler Z Physiol Chem 354:1351–1361

    PubMed  Google Scholar 

  19. Weber MH, Kyung-Sun Cheong, Schott KJ, Neuhoff V (1986) Microelectrophoresis in the differential diagnosis of proteinuric diseases. Electrophoresis 7:134–141

    Google Scholar 

  20. Schiwara HW, Hebell T, Kirchherr H, Postel W, Weser J, Görg A (1986) Ultrathin-layer sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis and silver staining of urinary proteins. Electrophoresis 7:496–505

    Google Scholar 

  21. Scherberich JE, Fischer P, Bigalke A, Stangl P, Wolf GB, Haimerl M, Schoeppe W (1989) Routine diagnosis with PhastSystem compared to conventional electrophoresis: automated sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining and Western blotting of urinary proteins. Electrophoresis 10:58–62

    PubMed  Google Scholar 

  22. Stierle HE, Oser B, Boesken WH (1990) Improved classification of proteinuria by semiautomated ultrathin SDS polyacrylamide gel electrophoresis. Clin Nephrol 33:168–173

    PubMed  Google Scholar 

  23. Brocklebank T, Cooper E, Richmond K (1991) SDS PAGE: patterns of proteinuria in various renal diseases of childhood. Pediatr Nephrol 5:371–375

    PubMed  Google Scholar 

  24. Fabre J, Eliades S, Favre H, Wauters JP (1970) Interet clinique de l'analyse des proteines urinaires. Praxis 59:1781–1787

    PubMed  Google Scholar 

  25. Manucl Y, Revillard JP, Betuel H (1970) Proteins in normal and pathological urine. Karger, Basel

    Google Scholar 

  26. Boesken WH (1976) Molekulargewichtsbezogene Analyse der Proteinurie als Beitrag zur Diagnose renaler Erkrankungen. Med Klin 71:441–448

    PubMed  Google Scholar 

  27. Pesce AJ, First MR (1979) Proteinuria. Dekker, New York

    Google Scholar 

  28. Stolte H, Alt J, Schurek HJ (1979) Experimentelle und klinische Untersuchungen zur Differentialdiagnostik der Proteinurie. Klin Wochenschr 57:1069–1079

    PubMed  Google Scholar 

  29. Ehrich JHH, Foellmer HG, Krull F, Winnecken HJ, Wurster U (1984) Aussagen, Fehlerquellen und Grenzen der Proteindiagnostik im Urin. I. Diagnostische Methoden. Monatsschr Kinderheilkd 132: 136–142

    PubMed  Google Scholar 

  30. Ehrich JHH, Foellmer HG, Krull F, Winnecken HJ, Wurster U (1984) Aussagen, Fehlerquellen und Grenzen der Proteindiagnostik im Urin. II. Normalwerte und Klassifikation der pathologischen Proteinurien. Monatsschr. Kinderheilkd 132:144–150

    PubMed  Google Scholar 

  31. Weber MH (1988) Review: urinary protein analysis. J Chromatogr 429:315–344

    PubMed  Google Scholar 

  32. Wurster U, Ehrich JHH (1985) Silberfärbung von Urinproteinen. Nieren Hochdruckkr 14:491–496

    Google Scholar 

  33. Burghard R, Galaske RG, Offner G, Ehrich JHH, Leititis JU, Brodehl J (1986) Urinary protein analysis in the early detection of acute rejection episodes after renal transplantation in children. Clin Nephrol 26:1–6

    PubMed  Google Scholar 

  34. Hoffmann A, Ehrich JHH, Wurster U (1989) Identification of urinary proteins after one-dimensional SDS-PAGE by immunoblotting and polychromatic silver stain. In: Radola BJ (ed) Electrophoresis forum'89. Technical University, Munich, pp 468–474

    Google Scholar 

  35. Doehring E, Ehrich JHH, Vester U, Feldemeir H, Poggensee U, Brodehl J (1985) Proteinuria, hematuria, and leukocyturia in children with mixed urinary and intestinal schistosomiasis. Kidney Int 28: 520–525

    PubMed  Google Scholar 

  36. Kaiser C, Doehring-Schwerdtfeger E, Ibrahim M, Daubner G, Vester U, Mamoun M Homeida, Osman Al-Hamour, Schmidt E, Mihatsch M, Ehrich JHH (1989) Renal function and morphology in Sudanese patients with advanced hepatosplenic schistosomiasis and portal hypertension. Am J Trop Med Hyg 40:176–185

    PubMed  Google Scholar 

  37. Mamoun Elsheikh, Doehring-Schwerdtfeger E, LKaiser C, Ibrahim M, Ghorashi M, Franke D, Porrath K, Kardorff R, Ehrich JHH (1989) Renal function in Sudanese school children withSchistosoma mansoni infection. Pediatr Nephrol 3:259–264

    PubMed  Google Scholar 

  38. Ehrich JHH, Wurster U, Lütge M, Mertz I, Doehring E, Oemar B (1986) Enzymurie und Nierenerkrankungen im Kindesalter. monatsschr Kinderheilkd 134:832–839

    PubMed  Google Scholar 

  39. Steinkamp G, Lütge M, Wurster U, Schulz-Baldes JG, Gröne HJ, Ehrich JHH (1986) Renal function in cystic fibrosis: proteinuria and enzymuria before and after tobramycin therapy. Eur J Pediatr 145: 526–531

    PubMed  Google Scholar 

  40. Wurster U, Patzold U, Ehrich JHH (1985) Assessment of enzymuria in multiple sclerosis patients treated with cyclosporin A. In: Bach PH, Lock EA (eds) Renal heterogeneity and target cell toxicity. Wiley, chichester, pp 457–460

    Google Scholar 

  41. Alt J, Maess B (1985) Konzentrierung von Urineiweissen mit Minicon B 15 Konzentratoren. Nieren Hochdruckkr 14:484–486

    Google Scholar 

  42. Alt JM, Jänig H, Schurek HJ, stolte H (1979) Study of renal protein excretion in chronic pyelonephritis. Contrib Nephrol 16:37–43

    PubMed  Google Scholar 

  43. Anderson NG, Anderson NL, Tollaksen SL, Hahn H, Giere F, Edwards J (1979) Analytical techniques for cell fraction. XXV. Concentration and two-dimensional electrophoretic analysis of human urinary proteins. Anal Biochem 95:48–61

    PubMed  Google Scholar 

  44. Hardwicke J (1970) Proteinuria. Scientific basis of medicine annual reviews. The Athlone Press, University of London, pp 211–229

  45. Boylan JW (1979) Introduction. Kidney Int 16:247–250

    Google Scholar 

  46. Hardwicke J (1984) Proteinuria — the future? Clin Nephrol 21: 50–53

    PubMed  Google Scholar 

  47. Hardwicke J (1981) Clinical significance of urinary albumin dimers in the nephrotic syndrome. Contrib Nephrol 24:72–75

    PubMed  Google Scholar 

  48. Lison AE, Halverscheidt U, Feldmann U, Meyer E (1985) Häufigkeit von renalen Proteinurien im Kindergartenalter. Nieren Hochdruckkr 14:512–514

    Google Scholar 

  49. Chambers RE, Bullock DG, Whichker JT (1989) Urinary total protein estimation — fact or fiction. Nephron 53:33–36

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ehrich, J.H.H., Wurster, U. Differentiation of proteinurias with electrophoresis. Pediatr Nephrol 5, 376–378 (1991). https://doi.org/10.1007/BF01453655

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01453655

Keywords

Navigation