Abstract
The effects of positive and negative air ions on the active and residual iron fractions of barley seedlings were studied during the course of iron chlorosis. Active iron is that fraction localized in the chloroplasts which dissolves in 1.0 N HC1 and participates in the biosynthesis of chlorophyll. Residual iron is not soluble in 1.0 N HC1 and is not concerned with the biosynthesis of chlorophyll. Air ions of either charge induced a significant decrease in active iron content which was associated with a decrease in chlorophyll content. Concomitantly there occurred an increase in both the residual iron and the cytochrome c fractions of the seedlings. There is evidence that the rise in residual iron content may involve not only cytochrome c but also other cytochromes and iron-containing enzymes as well. We have theorized that the site of air ion action in the experiments reported may be the regulatory systems controlling iron metabolism in the seed and young seedling. Through this action air ions apparently divert endogenous free-state iron from conversion to active iron and make it available for the production of a number of ironcontaining compounds which are components of the residual iron fraction.
Zusammenfassung
Die Wirkung von positiven und negativen Luftionen auf die “Aktiv”- und “Rest”-EisenfraktÏon in Gerstenkeimlingen wurde im Verlauf der Eisenchlorose untersucht. “Aktiv”-Eisen ist die Fraktion in dem FarbstofftrÄger, die in 1.0 N HC1 lösbar und an der Chlorophyllsynthese beteiligt ist. “Rest”-Eisen istnicht löslich in 1.0 N HC1 und an der Chlorophyllsynthese unbeteiligt. Positive und negative Luftionen bewirkten eine signifikante Verminderung des Gehalts an Aktiv-Fe und Chlorophyll. Gleichzeitig wurden höhere Anteile an Rest-Fe und Cytochrom c gefunden. Es sind Hinweise dafür vorhanden, dass die Zunahme des Rest-Fe nicht nur das Cytochrom c,sondern auch andere Fe-haltige Enzyme betrifft. Die Autoren nehmen an, dass die Luftionen an dem Regulationssystem angreifen, das den Eisenstoffwechsel der Samen und Keimlinge kontrolliert. Luftionen verhindern scheinbar die Umbildung von endogenem Fe-freier Form zu Aktiv-Fe und machen es für die Bildung einer Reihe von Fe-haltigen Verbindungen zugÄnglich, die in der Rest-Fe Fraktion enthalten sind.
Resume
Les effets de l'ionisation positive ou négative de l'air ont été étudiés fraction “active” et sur la fraction “résiduelle” du Fer contenu dans des germes d'orge au cours de la sidérochlorose. Le Fer actif est la fraction des chloroplastes soluble dans l'Acide Chlorhydrique 1,0 N et participant à la biosynthèse de la chlorophylle. Le Fer résiduel n'est pas soluble dans H C1 1,0 N et n'entre pas dans la biosynthèse de la chlorophylle. Les ions positifs et négatifs de l'air ont provoqué une réduction significative de la teneur en Fer actif et en chlorophylle. Simultanément, il est apparu un accroissement de Fer résiduel et du Cytochrome c. Certains indices suggèrent que l'accroissement du Fer actif porte non seulement sur le Cytochrome c mais aussi sur d'autres enzymes contenant du Fer. Les auteurs pensent que les ions de l'air agissent au niveau des systèmes de régulation du métabolisme du Fer dans les graines et dans les germes. Les ions de l'air détournent apparemment le Fer libre endogène de la conversion en Fer actif et le rendent disponible pour la formation d'un certain nombre de composés contenant du Fer qui entrent dans la fraction du Fer résiduel.
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Krueger, A.P., Kotaka, S. & Andriese, P.C. Studies on air-ion-enhanced iron chlorosis I. Active and residual iron. Int J Biometeorol 8, 5–16 (1964). https://doi.org/10.1007/BF02186923
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DOI: https://doi.org/10.1007/BF02186923