Pistil hyperplasia in rice spikelets as affected by heat stress

Summary

Sexual organogenesis in spikelets of rice (Oryza sativa L. cv ‘Kinmaze’) affected by heat stress was investigated using SEM and stereo-microscopy. Of the 243 spikelets dissected, 55.6% developed pistil hyperplasia, i.e., proliferated female organs or tissues, including multiple stigmata and/or ovaries, outgrowth of swollen parenchymatous tissues from inside the ovule, and differentiation of trichomes from ovary epidermis. Conversely, 7% of the spikelets exhibited stamen hypoplasia, represented by a decrease in the number of stamens, and only 3.7% of the spikelets showed hyperplasia, represented by an increase in the number of stamens. The morphological and structural development of the anther was disturbed, and microsporogenesis was inhibited. The shape of the anther was altered, though not perfectly, into the form of an ovary; the lemma and palea changed in form and ultimately resembled each other in shape and size. All of these changes in structure are more or less similar to those that usually result in rice spikelets subjected to cold and other environmental stresses. It was therefore concluded that rice plants show similar responses in sex differentiation in the spikelet under various environmental stresses.