Summary
The application of confocal laser scanning microscopy together with in situ hybridization experiments in tobacco pollen enabled a detailed localization of a pollen-specific mRNA. The three-dimensional distribution of this specific mRNA over the whole pollen grain was reconstructed by means of optical sections of one specimen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson MA, Cornish EC, Mau SL, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roche PJ, Haley JD, Penschow JD, Niall HD, Tregear GW, Coghlan JP, Crawford RJ, Clarke AE (1986) Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38–44
Angerer LM, Cox KH, Angerer RC (1987) Demonstration of tissue-specific gene expression by in situ hybridization. In: Berger S, Kimmel A (eds) Methods in enzymology, guide to molecular cloning techniques, vol 152. Academic Press, London New York pp 649–661
Amos WB, White JG, Fordham M (1987) Use of confocal imaging in the study of biological studies. Appl Opt 26:3239–3243
Buchen B, Sievers A (1981) Sporogenesis and pollen grain formation. In: Kiermayer O (ed) Cytomorphogenesis in plants. Springer, Berlin Heidelberg New York, pp 366–369
Budelier KM, Smith AG, Gasser ChS (1990) Regulation of a stylar transmitting tissue-specific gene in wild-type and transgenic tomato and tobacco. Mol Gen Genet 224:183–192
Clark KR, Okuley JJ, Collins PD, Sims TL (1990) Sequence variability and developmental expression of S-alleles in self-incompatible and pseudo-self-compatible Petunia. Plant Cell 2:815–826
Cox KH, DeLeon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101:485–502
Giles KL, Prakash J (eds) (1987) Pollen: cytology and development. Int Rev Cytol, vol 107. Academic Press, New York
Goldberg RB, Barker SJ, Perez-Grau L (1989) Regulation of gene expression during plant embryogenesis. Cell 56:149–160
Hanson DD, Hamilton DA, Travis JL, Bashe DM, Mascarenhas JP (1989) Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1:173–179
Harders J, Lukács N, Robert-Nicoud M, Jovin TM, Riesner D (1989) Imagion of viroids in nuclei from tomato leaf tissue by in situ hybridization and confocal laser scanning microscopy. EMBO J 8:3941–3949
Herpen MMA, Reijnen WH, Schrauwen JAM, de Groot PFM, Jager JWH, Wullems GJ (1989) Heat shock proteins and survival of germinating pollen of Lilium longtflorum and Nicotiana tabacum. J Plant Physiol 134:345–351
Houtsmuller AB, Oud JL, van der Voort HTM, Baarslag MW, Krol JJ, Mosterd B, Mans A, Brakenhoff GJ, Nanninga N (1990) Image processing techniques for 3-D chromosome analysis. J Microsc 158:235–248
Kuhlemeier C, Green PJ, Chua N-H (1987) Regulation of gene expression in higher plants. Annu Rev Plant Physiol 38:221–257
Maliga P, Sz-Breznovitis A, Marton L (1973) Streptomycin resistant plants from callus culture of haploid tobacco. Nature New Biol 244:29–30
Mariani C, de Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347:737–741
Mascarenhas JP (1988) Antherand pollen-expressed genes. In: Verma DPS, Goldberg RB (eds) Temporal and spatial regulation of plant genes. Springer, Berlin Heidelberg New York, pp 97–115
Mascarenhas JP (1990) Gene activity during pollen development. Annu Rev Plant Physiol Plant Mol Biol 41:317–338
McFadden GI (1989) In situ hybridization in plants: from macroscopic to ultrastructural resolution. Cell Biol Int Rep 13:3–21
Pawley JB (1990) Handbook of biological confocal microscopy. Plenum Press, New York
Schrauwen JAM, de Groot PFM, van Herpen MMA, van der Lee T, Reijnen WH, Weterings KAP, Wullems GJ (1990) Stage-related expression of mRNAs during pollen development in lily and tobacco. Planta 182:298–304
Shotton DM (1989) Confocal scanning optical microscopy and its applications for biological specimens. J Cell Sci 94:175–206
Weterings KAP (1991) Analysis of pollen-specific gene expression in tobacco. J Cell Biochem [Suppl 15A] 141
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reijnen, W.H., van Herpen, M.M.A., de Groot, P.F.M. et al. Cellular localization of a pollen-specific mRNA by in situ hybridization and confocal laser scanning microscopy. Sexual Plant Reprod 4, 254–257 (1991). https://doi.org/10.1007/BF00200544
Issue Date:
DOI: https://doi.org/10.1007/BF00200544