Skip to main content
SpringerLink
Log in

Resistenzsteigerung vom Mehrschrittyp bei in vitro wiederholt subinhibitorischen Konzentrationen von Zweitgenerationschinolonen ausgesetzten Neisseria gonorrhoeae-Isolaten

Decrease of antimicrobial susceptibility of gonococci to newer quinolones after repeated exposition to subinhibitory concentrations in vitro

  • Mikrobiologische Studien
  • Published:
Infection Aims and scope Submit manuscript

Zusammenfassung

Fünf rezente Gonokokkenisolate wurden 25mal in Flüssigmedien überimpft, welche subinhibitorische Konzentrationen verschiedener Antibiotika enthielten. In Gegenwart von Rifampicin wurden alle Stämme schnell resistent (Einschrittyp). In Gegenwart von Penicillin, Enoxacin sowie Ciprofloxacin konnte ebenfalls eine Resistenzsteigerung beobachtet werden. Diese ist jedoch offensichtlich dem langsameren Mehrschrittyp zuzuordnen. Bemerkenswert ist ferner, daß bei Ciprofloxacin, sicherlich mit bedingt durch die von vornherein niedrigere MHK, bei keinem der fünf untersuchten Keime in Gegenwart einer höheren Konzentration als 0,064 mg/l noch Wachstum nach 25 Kulturpassagen auftrat. Eine schnelle Resistenzentwicklung vonNeisseria gonorrhoeae gegenüber Enoxacin oder Ciprofloxacin, welche in der Gonorrhoetherapie eingesetzt werden, muß somit nicht erwartet werden.

Summary

Five recent gonoccal isolates were exposed to subinhibitory concentrations of several antibioticsin vitro 25 times. In the presence of rifampicin all strains quickly became resistant. In the presence of penicillin, enoxacin and ciprofloxacin, antimicrobial susceptibility also decreased. Here development of resistance, however, corresponded to the multi- and not to the one-step type. It seems remarkable that even at the end of the experiments no strain grew at concentrations of ciprofloxacin exceeding 0.064 mg/l. In conclusion, quick development of resistance need not be expected after the introduction of newer quinolones into the therapy of gonorrhoea on a large scale.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Campbell, D. J. Gonorrhoea in North Africa and central Mediterranean. Br. Med. J. 2 (1944) 44–47.

    Google Scholar 

  2. Korting, H. C., Beckenkamp, B. Repeated exposition ofNeisseria gonorrhoeae to subinhibitory concentrations of antibiotics in liquid medium leads to a fast increase of its resistance with rifampicin, but not with penicillin G, cefotiam or ceftizoxime. Eur. J. Sex. Transm. Dis. 3 (1986) 163–167.

    Google Scholar 

  3. Morse, S. H., Vaughan, P., Golluster, D., Iglewski, B. H. Inhibition ofNeisseria gonorrhoeae by a bacteriocin fromPseudomonas aeruginosa. Antimicrob. Agents Chemother. 10 (1976) 354–362.

    Google Scholar 

  4. Brookes, R., Heden, C.-G. Dense culture ofNeisseria gonorrhoeae in liquid medium. Appl. Microbiol. 15 (1967) 219–223.

    Google Scholar 

  5. Thornsberry, C., Hill, B. C., Swenson, J. M., McDougal, L. K. Rifampicin: spectrum of antibacterial activity. Rev. Infect. Dis. 5, Suppl. 3 (1983) 412–417.

    Google Scholar 

  6. Zinsser, R.: Appleton, century, crofts. Microbiology (1960) 91–96.

  7. Brown, S., Biddle, J., Warnnissorn, T., Panikakbutra, K., Traisuipa, A. Antimicrobial resistance ofNeisseria gonorrhoeae in Bangkok: Is single-drug treatment passé? Lancet 2 (1982) 1366–1368.

    Google Scholar 

  8. Klingeren van, B., Wagenvoort, J. H. T., Willigen van der, A. H., Vliet van, H. J. A., Michel, M. F. Resistance ofNeisseria gonorrhoeae to enoxacin. J. Antimicrob. Chemother. 16 (1986) 429.

    Google Scholar 

  9. Thabaut, A., Durosoir, J.-L., Saliou, P. La sensibilité deNeisseria gonorrhoeae aux antibiotiques: considérations méthodologiques. Bull. Org. Mond. Santé. 59 (1981) 561–566.

    Google Scholar 

  10. Marcuse, K., Hussels, U. Zur Frage der Penizillinempfindlichkeit der Gonokokken. Dermatol. Wochenschr. 130 (1954) 1031–1044.

    Google Scholar 

  11. Meyer-Rohn, J. Experimentelle Untersuchungen zur Antibiotika-Resistenz vonNeisseria gonorrhoeae. Hautarzt 9 (1954) 81–84.

    Google Scholar 

  12. Miller, C. P., Bohnhoff, J. Studies on the action of penicillin. IV. Development of penicillin resistance by gonococcus. Proc. Soc. Exp. Biol. Med. 60 (1945) 354–356.

    Google Scholar 

  13. Korting, H. C., Behrendt, H., Roth, K.-H., Neubert, U. Repeated exposition to subinhibitory concentrations of antibioticin vitro readily decreases susceptibility ofNeisseria genorrhoeae to rifampicin, but not to new cephalosporins and penicillin G. Chemotherapy 30 (1984) 366–372.

    Google Scholar 

  14. Hafiz, S., McEntegart, M. G. Prolonged survival ofNeisseria gonorrhoeae in a new liquid medium. Br. J. Vener. Dis. 52 (1976) 381–383.

    Google Scholar 

  15. Cobbold, R. J. C., Morrison, G. D., Willcox, R. R. Treatment of gonorrhoea with single oral doses of rifampicin. Br. Med. J. 4 (1968) 681–682.

    Google Scholar 

  16. Hopsu-Havu, V. K., Helander, I. Rifampicin in the treatment of acute gonorrhoea. Zeitschr. Hautkr. 47 (1972) 441–444.

    Google Scholar 

  17. Willigen van der, A. H., Hoek van der, J. C. S., Wagenvoort, J. H. T., Vliet van, H. J. A., Klingeren van, B., Schalla, W. O., Knapp, J. S., Joost van, T., Michel, M. F., Stolz, E. Comparative double-blind study of 200- and 400-mg enoxacin given orally in the treatment of acute uncomplicated urethral gonorrhoea in males. Antimicrob. Agents Chemother. 31 (1987) 535–538.

    Google Scholar 

  18. Notowicz, A., Stolz, E., Klingeren van, B. A double blind study comparing two dosages of enoxacin for the treatment of uncomplicated urogenital gonorrhoea. J. Antimicrob. Chemother. 14, Suppl. C (1984) 91–94.

    Google Scholar 

  19. Gombert, M. E. Thein vitro activity of quinolone antibiotics against pathogens causing sexually transmitted diseases. Infect. Med. Lett. Obstetr. Gyn. 8, suppl. (1986) 32–34.

    Google Scholar 

  20. Szarmach, H., Weuta, H., Podziewski, J., Szarzynski, J., Wronski, A. Ciprofloxacin in acute male gonorrhoea. Arzneimittelforsch. 36 (1986) 1840–1842.

    Google Scholar 

  21. Tegelberg-Stassen, M. J. A. M., Hoek van der, J. C. S., Mooi, L., Wagenvoort, J. H. T., Joost van, T., Michel, M. F., Stolz, E. Treatment of uncomplicated gonococcal urethritis in men with two dosages of ciprofloxacin. Eur. J. Clin. Microbiol. 5 (1986) 244–246.

    Google Scholar 

  22. Wise, R., Lister, D., McNulty, C. A. M., Griggs, D., Andrews, J. M. The comparative pharmacokinetics of five quinolones. J. Antimicrob. Chemother. 18, Suppl. D (1986) 71–81.

    Google Scholar 

  23. Jaffe, H. W., Schroeter, A. L., Reynolds, G. H., Zaidi, A. A., Martin, J. E., Thayer, J. D. Pharmacokinetic determinants of penicillin cure of gonococcal urethritis. Antimicrob. Agents Chemother. 15 (1979) 587–591.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dermatologische Klinik und Poliklinik der Ludwig-Maximilians-Universität München, Frauenlobstraße 9–11, D-8000, München 2

    H. C. Korting &  A. Lukacs

Authors
  1. H. C. Korting
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Lukacs
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Korting, H.C., Lukacs, A. Resistenzsteigerung vom Mehrschrittyp bei in vitro wiederholt subinhibitorischen Konzentrationen von Zweitgenerationschinolonen ausgesetzten Neisseria gonorrhoeae-Isolaten. Infection 17 (Suppl 1), S6–S10 (1989). https://doi.org/10.1007/BF01643626

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01643626

Search

Navigation