Skip to main content
SpringerLink
Log in

Leucocyte values in rats and mice following carbon dioxide euthanasia

  • Short Paper
  • Published:
Comparative Haematology International Aims and scope Submit manuscript

Abstract

Leucocyte counts obtained from Wistar rats and CD1 mice following euthanasia with carbon dioxide were compared to leucocyte values obtained using other agents for anaesthesia or euthanasia. In rats, following euthanasia with carbon dioxide, lymphocyte and neutrophil counts in samples taken from the heart were significantly raised compared with sodium barbiturate euthanasia. In mice, following carbon dioxide, total leucocyte counts from brachial plexus were significantly lower than other collection sites. Lymphocyte counts from vena cava and brachial plexus were also significantly lower than other sites.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adegh A J-H, Cohen J (1986) A better method for terminal bleeding of mice. Lab Anim 20:70–72.

    Google Scholar 

  • Archer RK, Riley J (1981) Standardised method for bleeding rats. Lab Anim 15:25–28.

    Google Scholar 

  • Blackshaw JK, Fenwick DC, Beattie AW, Allan DJ (1988) The behaviour of chickens, mice and rats during euthanasia with chloroform, carbon dioxide and ether. Lab Anim 22:67–75.

    Google Scholar 

  • Evans GO, Flynn RM (1990) Further observations on in-vitro aggregation of rat platelets with different collagens. Thromb Res 57:301–303.

    Google Scholar 

  • Fawell JK, Thomson C, Cooke L (1972) Respiratory artefact produced by carbon dioxide and pentobarbitone sodium euthanasia in rats. Lab Anim 6:321–326.

    Google Scholar 

  • Fower JSL, Brown JS, Flower EW (1980) Comparison between ether and carbon dioxide anaesthesia for removal of small blood samples from rats. Lab Anim 14:275–278.

    Google Scholar 

  • Howard HL, McLaughlin-Taylor E, Hill RL (1990) The effect of mouse euthanasia technique on subsequent lymphocyte proliferation and cell mediated lympholysis assays. Lab Anim Sci 40:510–514.

    Google Scholar 

  • Smith CN, Neptun DA, Irons RD (1968) Effect of sampling site and collection method on variations in baseline clinical pathology parameters in Fischer-344 rats. Fundam Appl Toxicol 7:658–663.

    Google Scholar 

  • Smith AW, Houpt KA, Kitchell RL, Kohn DF, McDonald LE, Passaglia M et al. (1986) Report of the AVMA panel on euthanasia. J Am Vet Med Assoc 188: 252–268.

    Google Scholar 

  • Weihe WH (1986) The laboratory rat In: Poole TB (ed) The U.F.A.W. handbook on the care and management of laboratory animals. Longman Scientific and Technical, London.

    Google Scholar 

  • Young L, Chambers TR (1973) A mouse bleeding technic yielding consistent volume with minimal hemolysis. Lab Anim Sci 23:428–430.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Drug Safety Evaluation, Wellcome Research Laboratories, BR3 3BS, Beckenham, Kent, UK

    G. O. Evans & D. E. C. Smith

Authors
  1. G. O. Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. E. C. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Evans, G.O., Smith, D.E.C. Leucocyte values in rats and mice following carbon dioxide euthanasia. Comparative Haematology International 1, 233–235 (1991). https://doi.org/10.1007/BF00235378

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00235378

Keywords

Search

Navigation