Skip to main content
SpringerLink
Log in

Pluripotent and committed haemopoietic progenitor cells in rat peripheral blood

  • Original Article
  • Published:
Comparative Haematology International Aims and scope Submit manuscript

Abstract

The assay system for determination of haemopoietic progenitors in peripheral blood of rats is essen tial for potential studies on mobilisation and transplantation of circulating progenitor cells in a rat experimental model. This paper demonstrates the possibility of detection and quantification of pluripotent progenitors (Colony Forming Units-Spleen day 8-CFU-Sd8) and committed progenitors (Colony Forming Units Granulocyte Macrophage-CFU-GM and Burst Forming Units-Erythroid-BFU-E) in peripheral blood of rats in a steady state. For determination of CFU-Sd8 the ‘rat to mouse’ in vivo assay was used, and for committed progenitors in vitro assays on methylcellulose were employed. The CFU-Sd8 incidence ranged from 7.3 to 11.6/ml of rat blood, similar to that reported in literature for mice. The incidence of CFU-GM was found to be 59.7 ± 9.4/ml which is in the range of the literature data for mice, rabbits, dogs and humans. The incidence of BFU-E in rat peripheral blood was 4.3 ± 1/ml, which was relatively low, but could be also considered as comparable with some literature data for dogs and humans. The CFU-E were not detected by the technique used. These results confirmed the existence of circulatory blood pluripotent progenitors (CFU-Sd8) and committed (CFU-GM and BFU-E) progenitors in rat, as has been established for some other mammalian species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barr RD, Whang-Peng J, Perry S (1975) Hematopoietic stem cells in human peripheral blood. Science 190:284–285

    Google Scholar 

  • Basara N, Biljanović-Paunović L, Pavlović-Kentera V (1988) Method for growing primitive erythroid progenitors (BFU-E) from rat bone marrow. Exp Hematol 16:790–793

    Google Scholar 

  • Biljanović-Paunović L, Ruvidić R, Pavlović-Kentera V (1990) Endobenous BFU-E in peripheral blood in diagnosis of polycythemia vera. Eur J Haematol 45:262–266

    Google Scholar 

  • Biljanović-Paunović L, Stojanovid N, Mostarica-Stojković M et al. (1992) Hematopoietic growth factors in anemia of Belgrade laboratory (b/b) rats. Exp Hematol 20:1257–1262

    Google Scholar 

  • Bolin RB, Stewart DA, Cheney BA et al. (1983) Granulocyte progenitor cell (CFUc) harvest by continuous apheresis in dogs. Effects of blood volume and lithium on yields. Exp Hematol 11:226–230

    Google Scholar 

  • Coggle JE, Gordon MY (1975) Quantitative measurements on the haemopoietic systems of three strains of mice. Exp Hematol 3:181–186

    Google Scholar 

  • Eridani S, Batten E, Sawyer B (1984) Erythroid colony formation in primary thrombocythaemia: evidence of hypersensitivity to erythropoietin. Br J Haematol 56:157–161

    Google Scholar 

  • Gerhartz HH, Fliedner TM (1980) Velocity sedimentation and cell cycle characteristics of granulopoietic progenitor cells (CFUC) in canine blood and bone marrow: influence of mobilization and CFUc depletion. Exp Hematol 8:209–218

    Google Scholar 

  • Gidáli J, Fehér (1985) The amount of mobilizable stem cells in perturbed hemopoiesis. Int J Cell Cloning 3:149–155

    Google Scholar 

  • Goodman JW, Hodgson GS (1962) Evidence for stem cells in the peripheral blood of mice. Blood 19:702–714

    Google Scholar 

  • Gratwohl A, Baldomero H, John L et al. (1995) Transplantation of GCSF mobilized allogeneic peripheral blood stem cells in rabbits. Bone Marrow Transplant 16:63–68

    Google Scholar 

  • Hara H, Ogawa M (1977) Erythropoietic precursors in murine blood. Exp Hematol 5:161–165

    Google Scholar 

  • Hendry JH (1971) The number of primary transplanted splenic colony-forming cells. Cell Tissue Kinet 4:217–221

    Google Scholar 

  • Iscove NN, Sieber F, Winterhalter KH (1974) Erythroid colony formation in cultures of mouse and human bone marrow. Analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin. Am J Cell Physiol 83:309–320

    Google Scholar 

  • Ivanović Z, Milenković P (1995a) The number and proliferation activity of rat bone marrow spleen colony forming-cells as determined in a ‘rat to mouse’ assay. Acta Vet (Beograd) 45:261–268

    Google Scholar 

  • Ivanović Z, Milenković P (1995b) The seeding efficiency of normal and hereditarily anemic (b/b) rat bone marrow colony forming units-spleen as determined in a ‘rat to mouse’ assay. Stem Cells 13:666–670

    Google Scholar 

  • Ivanović Z, Milenković P, Stojanović N et al. (1993) A stimulator of proliferation of spleen colony-forming cells (CFU-S) in the bone marrow of irradiated rats. Acta Vet (Beograd) 43:95–104

    Google Scholar 

  • Ivanović Z, Milenković P, Vasiljevska M et al. (1995a) Hematopoietic stem cells in the hereditarily anemic Belgrade laboratory (b/b) rat. Exp Hematol 23:1218–1223

    Google Scholar 

  • Ivanović Z, Milenković P, Stošić-Grujičić S (1995b) Constitutive production of regulators of stem cell proliferation in the hereditarily anaemic Belgrade laboratory (b/b) rat. Comp Haematol Int 5:170–176

    Google Scholar 

  • Ivanović Z, Milenković P, Zarić J et al. (1995c) The effect of hemin or iron treatment on bone marrow and spleen hemopoietic stem cells of hereditarily anemic Belgrade laboratory (b/b) rats. Exp Hematol 23:812 (Abstr.)

    Google Scholar 

  • Jovčić G, Stojanović N, Kataranovski M et al. (1993) Acute sterile inflammation-correlation between cellular changes and extramedullary-produced regulators in vivo. Ann Hematol 66:195–201

    Google Scholar 

  • Kellar K, Rolović Z, Evatt BL et al. (1985) Characteristics of rat megakaryocyte colonies and their progenitors in agar culture. Exp Hematol 13:1018–1024

    Google Scholar 

  • Körbling M, Fliedner TM, Calvo W et al. (1979) Albumin density gradient purification of canine hemopoietic blood stem cells (HBSC): long-term allogeneic engraftment without GVH-reaction. Exp Hematol 7:277–288

    Google Scholar 

  • Kreja L, Baltschukat K, Nothdruft W (1988) Growth of erythoroid burst-forming units (BFU-E) in cultures of canine bone marrow and peripheral blood cells: effect of serum from irradiated dogs. Exp Hematol 16:647–651

    Google Scholar 

  • Lacombe C, Casadevall N, Varet B (1980) Polycythaemia vera: in vitro studies of circulating erythroid progenitors. Br J Haematol 44:189–199

    Google Scholar 

  • Lahiri SK, van Putten LM (1969) Distribution and multiplication of colony forming units from marrow and spleen after injection in irradiated mice. Cell Tissue Kinet 2:21–27

    Google Scholar 

  • Lee JH, Klein HG (1995) The effect of donor red cell sedimentation rate on efficiency of granulocyte collection by centrifugal leukapheresis. Transfusion 35:384–388

    Google Scholar 

  • Lord BI, Woolford LB, Wood LM et al. (1995) Mobilization of early hematopoietic progenitor cells with BB-10010: a genetically engineered variant of human macrophage inflammatory protein —1a. Blood 85:3412–3415

    Google Scholar 

  • Majolino I, Buscemi F, Scimé R et al. (1995) Treatment of normal donors with rhG-CSF 16 µg/kg for mobilization of peripheral blood stem cells and their apheretic collection for allogeneic transplantation. Haematologica 80:219–226

    Google Scholar 

  • Martens ACM, van Bekkum DW, Hagenbek A (1986) Heterogeneity within the spleen colony-forming cell population in rat bone marrow. Exp Hematol 14:714–718

    Google Scholar 

  • Meagher RC, Rothmann SA, Paik In-Ki et al. (1989) In vitro growth of rat bone marrow BFU-E. Exp Hematol 17:374–378

    Google Scholar 

  • Metclaf D, Moore MAS (1971) Haemopoietic cells. North Holland, Amsterdam-London, p. 71.

    Google Scholar 

  • Milenković P, Ivanović Z, Kataranovski M et al. (1991) Stimulator of proliferation of spleen colony-forming cells in T-cell deprived mice treated with cyclophosphamide or irradiation. Cell Prolif 24:507–515

    Google Scholar 

  • Milenković P, Ivanović Z, Lukić ML et al. (1993) Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation. Cell Prolif 26:503–510

    Google Scholar 

  • Pavlović-Kentera V, Basara N, Jerkić M (1988) Erythrocytosis in spontaneously hypertensive rats. Exp Hematol 16:950–953

    Google Scholar 

  • Pavlović-Kentera V, Basara N, Biljanović-Paunović L et al. (1989) Erythroid progenitors in anemic Belgrade laboratory (b/b) rat. Exp Hematol 17:812–815

    Google Scholar 

  • Pavlović-Kentera V, Varagić V, Clemons G et al. (1992) The effect of aminophylline on erythropoietin production in rats. Iugoslav Physiol Pharmacol Acta 28:191–196

    Google Scholar 

  • Praloran V, Klausman M, Naud MF et al. (1989) Blood erythroid progenitors (CFU-E and BFU-E) in acute lymphoblastic leukemias. Blut 58:75–78

    Google Scholar 

  • Rubin SH, Cowan DH (1973) Assay of granulocytic progenitor cells in human peripheral blood. Exp Hematol 1:127–131

    Google Scholar 

  • Sheridan JW, Metcalf D (1973) A low-molecular-weight factor in lung-conditioned medium stimulating granulocyte-monocyte formation in vitro. J Cell Physiol 81:11–24

    Google Scholar 

  • Smith JE (1983) Comparative hematology. In: Williams WJ, Beutler E, Erslev A, Lichtman MA (eds) Hematology, McGraw-Hill, New York, p 117–125

    Google Scholar 

  • Stojanović N, Jovčić G, Basara N et al. (1990) Granulopoiesis in anemic Belgrade laboratory (b/b) rats. Exp Hematol 18:379–383

    Google Scholar 

  • Tambourin PE, Wendling F, Gallien-Lartigue O et al. (1973) Production of high levels of erythropoietin in mice. Biomedicine 19:112–116

    Google Scholar 

  • Tennant GB, Jacobs A, Bailey-Wood R (1986) Peripheral blood granulocyte-macrophage progenitors in patients with the myelodysplastic syndromes. Exp Hematol 14:1063–1068

    Google Scholar 

  • van Bekkum DW (1977) The appearance of the multipotential hemopoietic stem cell. In: Baum JB, Ledney GD (eds) Experimental hematology today. Springer-Verlag, New York, p 3

    Google Scholar 

  • Verma DS, Spitzer G, Zander R (1980) The myeloid progenitor cell: a parallel study of subpopulations in human marrow and peripheral blood. Exp Hematol 8:32–44

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Medical Research, Belgrade, Yugoslavia

    Z. Ivanovic, M. Petakov, G. Jovčić & P. Milenković

  2. Military Medical Academy, Belgrade, Yugoslavia

    B. Balint

Authors
  1. Z. Ivanovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Petakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Jovčić
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Biljanović-Paunović
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Balint
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Milenković
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ivanovic, Z., Petakov, M., Jovčić, G. et al. Pluripotent and committed haemopoietic progenitor cells in rat peripheral blood. Comparative Haematology International 7, 1–6 (1997). https://doi.org/10.1007/BF01320992

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01320992

Keywords

Search

Navigation