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Histochemische Untersuchung der Wirkung von Pharmaka auf die Aufnahme vonα-Methylnoradrenalin in Noradrenalin- und Dopamin-Neuren bei der reserpinisierten Ratte

Histochemical study of the effect of drugs on the uptake of α- methylnoradrenaline by noradrenaline and dopamine containing neurones in rats treated with reserpine

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Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

A number of drugs were tested in histochemical studies using a fluorescent technique in order to establish whether they inhibited the uptake ofα-methylnoradrenaline in catecholamine neurones in the reserpine-treated rat in vivo. The noradrenaline axons in the iris, mesenteric veins, and vas deferens were investigated, as well as the dopamine axons of the eminentia mediana of the hypothalamus. In the noradrenaline neurones an inhibitory action was exerted by the following substances (listed in descending order of effectiveness): desipramine, guanethidine, methylphenidate, cocaine, 1-(3-methylaminopropyl)-dibenzo [b, e] bicyclo [2.2.2] octadiene (34′276-Ba, a new antidepressive from the series of the dibenzo-bicyclo-octadienes), and tripelennamine. Of these substances only methylphenidate, cocaine, and tripelennamine exerted an inhibitory effect in the dopamine neurones. Two other compounds that were studied, the psycho-active drug benz-octamine (a tranquillizer from the series of the dibenzo-bicyclo-octadienes) and the monoamine oxidase inhibitor N-methyl-N-(2-propynyl)-1-indanamme (11′739-Su) did not exert an inhibitory effect either in the noradrenaline or in the dopamine neurones. Possible correlations between apparent inhibition of uptake and depletion ofα-methylnoradrenaline are discussed.

Zusammenfassung

Die Hemmwirkung verschiedener Pharmaka auf die Aufnahme vonα-Methylnoradrenalin (MNA) in Katecholamin-Neuren wurde fluorescenz-histochemisch an der reserpinisierten Ratte in vivo untersucht. Geprüft wurden die Noradrenalin-Axone in Iris, Mesenterialvenen und Vas deferens sowie die Dopamin-Axone der Eminentia mediana des Hypothalamus. In den Noradrenalin-Neuren hemmen mit abnehmender Wirksamkeit folgende Substanzen: Desipramin, Guanethidin, Methylphenidat, Cocain, 1-(3-Methylaminopropyl)-dibenzo [b, e] bioyclo [2.2.2] octadien (34′276-Ba, ein neues Antidepressivum aus der Reihe der Dibenzo-bicyclo-octadiene) und Tripelennamin. In den Dopamin-Neuren hemmen von diesen Substanzen nur Methylphenidat, Cocain und Tripelennamin. Zwei weitere untersuchte Verbindungen, das Psychopharmakon Benzoctamin (ein Tranquillizer aus der Reihe der Dibenzo-bicyclo-octadiene) und der Monoaminoxydasehemmer N-methyl-N-(2-propinyl)-1-indanamin (11′739-Su) hemmen weder in den Noradrenalin- noch in den Dopamin-Neuren. Mögliche Beziehungen zwischen apparenter Aufnahmehemmung und Entleerung von MNA werden diskutiert.

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Abbreviations

NA:

Noradrenalin

DA:

Dopamin

MNA:

α-Methylnor-adrenalin

Dopa:

3,4-Dihydroxyphenylalanin

KA:

Katecholamin(e)

ZNS:

Zentralnervensystem

i.p.:

intraperitoneal

i.v.:

intravenös

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  1. Pharmazeutische Abteilung, Forschungslaboratorien der CIBA-Aktiengesellschaft Basel, Schweiz

    H. -P. Lorez

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Lorez, H.P. Histochemische Untersuchung der Wirkung von Pharmaka auf die Aufnahme vonα-Methylnoradrenalin in Noradrenalin- und Dopamin-Neuren bei der reserpinisierten Ratte. Z. Gesamte Exp. Med. 151, 241–260 (1969). https://doi.org/10.1007/BF02044567

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  • DOI: https://doi.org/10.1007/BF02044567

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