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Differential enzymatic behaviour of single proximal segments of the superficial and juxtamedullary nephron

II. Lactic-, Isocitric-, Malic-, Glutamic- and Glucose-6-Phosphate dehydrogenases

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Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

With oxidoreductases mediating several metabolic pathways, e.g. LDH (glycolysis), ICDH, MDH (tricarboxylic acid cycle), GLDH (aminoacid metabolism) and G-6-PDH (hexose monophosphate shunt) additional marked differences between the subcapsular (SCN) and juxtamedullary (JMN) nephron of rat kidney could be established using quantitative histochemical technique. The enzymatic differences between the two nephron types are mainly quantitative as could be shown within the logarithmic network. The intrarenal enzymatic heterogeneity seems to constitute a general concept.

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Abbreviations

LDH:

lactic dehydrogenase EC 1.1.1.27

ICDH:

isocitric dehydrogenase EC 1.1.1.42

MDH:

malate dehydrogenase EC 1.1.1.37

G-6-PDH:

glucose-6-phosphate dehydrogenase EC 1.1.1.49

GLDH:

glutamic dehydrogenase EC 1.4.1.3

a.P'tase:

alkaline phosphatase EC 3.1.3.1

Na K ATPase:

sodium and potassium activated adenosintriphosphatase EC 3.6.1.3

SCN:

subcapsular nephron

JMN:

juxtamedullary nephron

G:

glomerulus

PTC:

proximal convoluted portion

PTR:

proximal straight portion

NAD(NADH):

nicotinamide-adenine dinucleotide

NADP(NADPH):

nicotinamide-adenine dinucleotide phosphate

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Authors and Affiliations

  1. Enzyme Laboratory, Medical Policlinic of the University of Basle, Switzerland

    U. Schmidt & U. C. Dubach

  2. Institute of Pathology, University of Tübingen, Germany

    U. Schmidt & U. C. Dubach

Authors
  1. U. Schmidt
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  2. U. C. Dubach
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With the technical assistance of Mrs. I. Bieder and Miss M. Kirstein.

Grants SNF No. 3.207.69 and DFG.

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Schmidt, U., Dubach, U.C. Differential enzymatic behaviour of single proximal segments of the superficial and juxtamedullary nephron. Z. Gesamte Exp. Med. 155, 340–353 (1971). https://doi.org/10.1007/BF02053466

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  • DOI: https://doi.org/10.1007/BF02053466

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