Summary
Primary tumors of the nervous system in rats induced by weekly administration of 6 mg/kg N-methylnitrosourea and spontaneous human brain tumors were investigated comparatively using immunoprecipitation technique. It is shown that two proteins antigenically identical with the specific glia proteins S100 and 14.3.2 are present in spontaneous as well as in experimental gliomas and schwannomas. These results demonstrate that the model of experimental tumors of the nervous system in rats is practicable for comparative morphological and biochemical studies of tumors of glial and schwann cell origin.
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References
Benda, P.: Protein — S-100 et tumeurs cerebral humaines. Soc. franc. Neurol.118, 368–372 (1968).
—, Lightbody, J., Sato, G., Levine, L., Sweet, W.: Differentiated rat glial cell strain in tissue culture. Science161, 370–371 (1968).
Bennet, G. S., Edelman, G. M.: Isolation of an acidic protein from rat brain. J. biol. Chem.243, 6234–6251 (1968).
Cicero, T. J., Cowan, W. M., Moore, B. W., Suntzeff, V.: The cellular localisation of the two brain specific proteins S-100 and 14.3.2. Brain Res.18, 25–34 (1969).
Davis, B. J.: Disc electrophoresis II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci.121, 404–427 (1969).
Haglid, K. G., Carlsson, C. A.: An immunological study of some human brain tumors concerning the brain specific protein S 100. Neurochirurgia (Stuttg.)14, 24–27 (1971).
--An immunological study of human brain tumors concerning the brain specific proteins S 100 and 14.3.2. J. Neurosurg. (in press).
Hydén, H., McEwen, B.: A glial protein specific for the nervous system. Proc. nat. Acad. Sci. (Wash.)55, 354–358 (1966).
Moore, B. W.: A soluble protein characteristic of the nervous system. Biochem. biophys. Res. Commun.19, 739–744 (1965).
—, McGregor, D.: Chromatografic and electrophoretic fractionation of soluble proteins of brain and liver. J. biol. Chem.240, 1647 (1965).
—, Perez, V. J.: Specific acidic proteins of the nervous system. In: Carlson, F. D. (Ed.), Physiological and Biochemical Aspects of Nervous Integration. Englewood Cliffs (N.J.): Prentice-Hall 1968.
Müller, W., Schröder, R.: Zur Diagnostik der Gliome. Neurochirurgia (Stuttg.)11, 30–36 (1968).
Ouchterlony, O.: In vitro method for testing toxinproducing capacity of diphteria. Acta path. microbiol. scand.25, 186–191 (1948).
Perez, V. J., Moore, B. W.: Wallerian degeneration in rabbit tibial nerve. Changes in the amounts of the S 100 protein. J. Neurochem.15, 971–977 (1968).
—, Olney, J. W., Cicero, T. J., Moore, B. W., Bahn, B. A.: Wallerian degeneration in rabbit optic nerve: cellular localisation in the central nervous system of the S 100 and 14.3.2 proteins. J. Neurochem.17, 511–519 (1970).
Zülch, K. J.: Biologie und Pathologie der Hirngeschwülste. Hdb. d. Neurochirurgie Bd. III. Berlin-Göttingen-Heidelberg: Springer 1956.
—, Wechsler, W.: Pathology and classification of gliomas. In: H. Krayenbühl, P. E. Maspes and W. H. Sweet (Eds.), Progress in Neurological Surgery, p. 49. Basel-New York: Karger 1968.
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Stavrou, D., Haglid, K.G. & Weidenbach, W. The brain specific proteins S100 and 14.3.2 in experimental brain tumors of the rat. Z. Gesamte Exp. Med. 156, 237–242 (1971). https://doi.org/10.1007/BF02047353
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DOI: https://doi.org/10.1007/BF02047353