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Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis

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Summary

Cathepsin D was purified from rat liver using a new affinity chromatographic method, based on the coupling to the specific inhibitor pepstatin. This preparation was used for the production of specific antibodies from rabbit. The purified IgG fraction was conjugated to horseradish peroxidase in a two-step coupling procedure and used for electron microscopic immunohistochemistry of the odontoblast-predentine region of the rat incisor. Precipitates, indicating the presence of cathepsin D, were seen in the odontoblast, odontoblast process, and in the extracellular unmineralized matrix, the predentine. The observations are discussed in relation to proteoglycan degradation at the mineralization front simultaneous with crystal formation, and in relation to the function of lysosomal enzymes in the turnover of connective tissue.

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References

  1. Dingle, J.T., Barrett, A.J., Weston, P.D.: Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown, Biochem. J.123:1–13, 1971

    PubMed  CAS  Google Scholar 

  2. Dingle, J.T., Barrett, A.J., Poole, A.R., Stovin, P.: Inhibition by pepstatin of human cartilage degradation, Biochem. J.127:443–444, 1972

    PubMed  CAS  Google Scholar 

  3. Woessner, J.F.: Purification of cathepsin D from cartilage and uterus and its action on the protein-polysaccharide complex of cartilage, J. Biol. Chem.248:1634–1642, 1972

    Google Scholar 

  4. Vaes, G., Jacques, P.: Studies on bone enzymes: the assay of acid hydrolases and other enzymes in bone tissue, Biochem. J.97:380–388, 1965

    CAS  Google Scholar 

  5. Vaes, G., Jacques, P.: Studies on bone enzymes: distribution of acid hydrolases, alkaline phenylphosphatase, cytochrome oxidase and catalase in subcellular fraction of bone tissue homogenates, Biochem. J.97:389–392, 1965

    CAS  Google Scholar 

  6. Vaes, G.: Hyaluronidase activity in lysosomes of bone tissue, Biochem. J.103:802–804, 1967

    PubMed  CAS  Google Scholar 

  7. Suga, S.: Histochemical observation of proteolytic enzyme activity in the developing dental hard tissue of rat, Arch. Oral Biol.15:555–558, 1970

    Article  PubMed  CAS  Google Scholar 

  8. Baylink, D., Wergedal, J., Thompson, E.: Loss of protein-polysaccharides at sites where bone mineralization is initiated, J. Histochem. Cytochem.20:279–292, 1972

    PubMed  CAS  Google Scholar 

  9. Engström, C., Linde, A., Persliden, B.: A fluorimetric study ofβ-glucuronidase andβ-galactosaminidase from the pulp of the rat incisor, Arch. Oral Biol.17:1421–1430, 1972

    Article  PubMed  Google Scholar 

  10. Engström, C., Linde, A., Persliden, B.: Acid hydrolases in the odontoblast-predentin region of dentinogenically active teeth, Scand. J. Dent. Res.84:76–81, 1976

    PubMed  Google Scholar 

  11. Hjerpe, A., Engfeldt, B.: Proteoglycans of dentine and predentine Calcif. Tissue Res.22:173–182, 1976

    Article  PubMed  CAS  Google Scholar 

  12. Linde, A., Persliden, B.: Cathepsin D activity in isolated odontoblasts, Calcif. Tissue Res.23:33–38, 1977

    Article  PubMed  CAS  Google Scholar 

  13. Linde, A., Persliden, B., Rönnbäck, L.: Cathepsin D: Purification from rat liver and immunohistochemical demonstration in rat incisor, Acta Odont. Scand.36:117–126, 1978

    PubMed  CAS  Google Scholar 

  14. Campo, R.D., Dziewiatkowski, D.D.: Turnover of the organic matrix of cartilage and bone as visualized by autoradiography, J. Cell. Biol.18:19–29, 1963

    Article  PubMed  CAS  Google Scholar 

  15. Fiore-Donno, G., Baume, L.-J.: Etude histochemique de la dentinogenèse humaine, Helv. Odont. Acta10:141–185, 1966

    CAS  Google Scholar 

  16. Hirschman, A., Dziewiatkowski, D.D.: Protein-polysaccharide loss during endochondral ossification: Immunochemical evidence, Science154:393–395, 1966

    PubMed  CAS  Google Scholar 

  17. Höhling, H.J., Nicholson, W.A.P., Schreiber, J., Zessack, U.: The distribution of some elements in predentine and dentine of rat incisor, Naturwissenschaften59:423, 1972

    Article  PubMed  Google Scholar 

  18. Pugliarello, M.C., Vittur, F., de Bernard, B., Bonucci, E., Ascenzi, A.: Chemical modifications in osteones during calcification, Calcif. Tissue Res.5:108–114, 1970

    Article  PubMed  CAS  Google Scholar 

  19. Lennox, D.W., Provenza, D.V.: Mucopolysaccharides in odontogenesis. Histochemical and autoradiographic study, Histochemie23:328–341, 1970

    Article  PubMed  CAS  Google Scholar 

  20. Laurent, T.C.: The ultrastructure and physical-chemical properties of interstitial connective tissue, Pflügers Arch.336:S 21-S 33, 1972

    Article  Google Scholar 

  21. Campbell, D.H., Garvey, J.S., Cremer, N.E., Sussdorf, D.H.: Immunoelectrophoresis. In: Methods in Immunology, pp. 149–155. W.A. Benjamin, New York, 1963

    Google Scholar 

  22. Linde, A., Persliden, B.: Purification of cathepsin D by AH-Sepharose affinity chromatography, Prep. Biochem.8:231–240, 1978

    PubMed  CAS  Google Scholar 

  23. Avrameas, S., Ternunck, T.: Peroxidase labeled antibody and Fab conjugates with enhanced intracellular penetration, Immunochemistry8:1175–1179, 1971

    Article  PubMed  CAS  Google Scholar 

  24. Molin, S.-O., Nygren, H., Dolonius, L.: A new method for the study of glutaraldehyde-induced crosslinking properties in proteins with special reference to the reaction with aminogroups, J. Histochem. Cytochem.26:412–414, 1978

    PubMed  CAS  Google Scholar 

  25. Boorshma, D.M., Kalsbeck, G.L.: A comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method, J. Histochem. Cytochem.23:200–207, 1975

    Google Scholar 

  26. Linde, A.: A method for the biochemical study of enzymes in the rat odontoblasts during dentinogenesis, Arch. Oral Biol.15:1209–1212, 1972

    Article  Google Scholar 

  27. Poole, A.R., Hembry, R.M., Dingle, J.T.: Extracellular localization of cathepsin D in ossifying cartilage, Calcif. Tissue Res.12:313–321, 1973

    Article  PubMed  CAS  Google Scholar 

  28. Dingle, J.T., Fell, H.B., Glauert, A.M.: Endocytosis of sugars in embryonic skeletal tissues in organ culture, J. Cell. Sci.4:139–154, 1969

    PubMed  CAS  Google Scholar 

  29. Dingle, J.T.: Secretion of enzymes into the pericellular environment, Philos. Trans. R. Soc. Lond.271:315–324, 1975

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Oral Biology, Department of Histology, University of Göteborg, Fack, S-400 33, Göteborg, Sweden

    H. Nygren, B. Persliden, H-A. Hansson & A. Linde

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  1. H. Nygren
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  2. B. Persliden
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  3. H-A. Hansson
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  4. A. Linde
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Nygren, H., Persliden, B., Hansson, HA. et al. Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis. Calcif Tissue Int 29, 251–256 (1979). https://doi.org/10.1007/BF02408088

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  • DOI: https://doi.org/10.1007/BF02408088

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