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Insulin enhances the growth of cartilage in organ and tissue cultures of mouse neonatal mandibular condyle

  • Laboratory Investigations
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Summary

Condylar cartilages were cultured in the form of organ cultures on top of collagen sponges in medium containing 2% fetal calf serum and were treated with 3.5–350 nM insulin for 6 days. Doses of 175 nM of insulin caused a marked increase (+96%) in DNA synthesis and in proteoglycan production (+74%), features that manifested themselves structurally by a 60% increase in overall size of the cultured explants. Using a tissue culture system comprised of cartilage progenitor cells, insulin was found to enhance the differentiation of the progenitor cells so that by 6 days in culture and appreciable nodule of differentiated chondrocytes developed. The latter was surrounded by perichondrial cells whereas the extracellular matrix within the newly formed, insulin-induced, nodule reacted positively for cartilagespecific antigens (type II collagen and bone sialoprotein). It is suggested that insulin induces a direct stimulatory effect on progenitor cell proliferation, cartilage differentiation, and extracellular matrix deposition.

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Authors and Affiliations

  1. Laboratory for Musculoskeletal Research, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O.B. 9649, 31096, Haifa, Israel

    Gila Maor & Michael Silbermann

  2. Research Unit for Clinical Rheumatology, Max-Planck Society, University of Erlangen-Nurnberg, Germany

    Klaus von der Mark

  3. Department of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

    Dick Heingard

  4. Institute of Pediatric and Adolescent Endocrinology, Beilinson Medical Center, Tel Aviv University, Petah Tikvah, Israel

    Zvi Laron

Authors
  1. Gila Maor
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  2. Michael Silbermann
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  3. Klaus von der Mark
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  4. Dick Heingard
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  5. Zvi Laron
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Maor, G., Silbermann, M., von der Mark, K. et al. Insulin enhances the growth of cartilage in organ and tissue cultures of mouse neonatal mandibular condyle. Calcif Tissue Int 52, 291–299 (1993). https://doi.org/10.1007/BF00296654

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  • DOI: https://doi.org/10.1007/BF00296654

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