Summary
Three cancer cell strains that fail to make permeable membrane junctions were tested for ability to transfer an endogenous hypoxanthine derivative from cell to cell. The cells of these strains, loaded with3H-hypoxanthine, were grown in contact with cells from a mutant line incapable of incorporating exogenous hypoxanthine. The transfer of the3H-hypoxanthine derivative to the mutant cells was determined by radio-autography and, in the same preparations, the presence of permeable membrane junctions was determined by intercellular fluorescein tracer diffusion and electrical measurement. The cells of the three strains showed no transfer of hypoxanthine derivative to contiguous mutant cells; the cells that make permeable junctions did show such transfer, under the same conditions.
In contrast to this contact-requiring mode of transfer, a contact-independent transfer phenomenon was observed with these three cancer cell strains.
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References
Adams, R. L. P. 1969. Phosphorylation of tritiated thymidine by L929 mouse fibroblasts.Exp. Cell Res. 56:49.
Azarnia, R., Loewenstein, W. R. 1971. Intercellular communication and tissue growth. V. A cancer cell strain that fails to make permeable membrane junctions with normal cells.J. Membrane Biol. 6:368.
Borek, C., Higashino, S., Loewenstein, W. R. 1969. Intercellular communication and tissue growth. IV. Conductance of membrane junctions of normal and cancerous cells in culture.J. Membrane Biol. 1:274.
Cox, R. P., Krauss, M. R., Balis, M. E., Dancis, J. 1970. Evidence for transfer of enzyme product as the basis of metabolic cooperation between tissue culture fibroblasts of Lesch-Nyhan Disease and normal cells.Proc. Nat. Acad. Sci. 67:1573.
Ham, R. G. 1965. Clonal growth of mammalian cells in a chemically defined, synthetic medium.Proc. Nat. Acad. Sci. 53:288.
Loewenstein, W. R. 1971. Cellular communication through membrane junctions. Symposium on Membranes.Arch. Intern. Med. 129:305.
Loewenstein, W. R., Kanno, Y. 1964. Studies on an epithelial (gland) cell junction. I. Modifications of surface membrane permeability.J. Cell Biol. 22:565.
MacPherson, J., Stoker, M. 1962. Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence.Virology 16:147.
Michalke, W., Loewenstein, W. R. 1971. Communication between cells of different types.Nature 232:121.
Miller, O. L., Stone, G. E., Prescott, D. M. 1964. Autoradiography of soluble materials.J. Cell Biol. 23:654.
Morris, H. P. 1965. Studies on the development, biochemistry and biology of experimental hepatomas.Advanc. Cancer Res. 9:227.
Pitts, J. D. 1971. Molecular exchange and growth control in tissue culture.In: Ciba Foundation Symposium on Growth Control in Cell Cultures. G. E. W. Wolstenholme and J. Knight, editors. p. 89. Churchill Livingston, London.
Potter, D. D., Furshpan, E. J., Lennox, E. S. 1966. Connections between cells of the developing squid as revealed by electrophysiological methods.Proc. Nat. Acad. Sci. 55:328.
Stoker, M. G. P. 1967. Transfer of growth inhibition between normal and virus-transformed cells: Radioautographic studies using marked cells.J. Cell Sci. 2:293.
Stoker, M., MacPherson, J. 1964. Syrian hamster fibroblast cell line BHK 21 and its derivatives.Nature 203:1355.
Subak-Sharpe, H. 1965. Biochemically marked variants of the Syrian hamster fibroblast cell line BHK 21 and its derivatives.Exp. Cell Res. 38:106.
Subak-Sharpe, H., Bürk, R. R., Pitts, J. D. 1966. Metabolic cooperation by cell to cell transfer between genetically different mammalian cells in tissue culture.Heredity 21:342.
Subak-Sharpe, H., Bürk, R. R., Pitts, J. D. 1969. Metabolic cooperation between biochemically marked mammalian cells in culture.J. Cell Sci. 4:353.
Vogt, M., Dulbecco, R. 1960. Virus-cell interaction with a tumor-producing virus.Proc. Nat. Acad. Sci. 46:365.
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Azarnia, R., Michalke, W. & Loewenstein, W.R. Intercellular communication and tissue growth. J. Membrain Biol. 10, 247–258 (1972). https://doi.org/10.1007/BF01867858
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DOI: https://doi.org/10.1007/BF01867858