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Effect of bicarbonate, pH, methazolamide and stibenes on the intracellular potentials of cultured bovine corneal endothelial cells

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Summary

Micropuncture of cultured bovine corneal endothelial cells led to registrations stable for hours. Intracellular potentials were mainly in the range of −40 to −55 mV, average 46.3±0.6 mV (sem). Changes of extracellular [HCO −3 ] led to voltage transients, their amplitude depending logarithmically on [HCO −3 ] with a mean slope of 37.3±8.8 (sd) mV. After removal of bicarbonate/CO2, a steady-state depolarization was seen. This steady-state depolarization, but not the voltage transients, could be reduced by 1mm Ba++. After removal of bicarbonate, the voltage response to changes of extracellular potassium was reduced. Alteration of pH i induced by permeable buffers (butyrate, glycodiazine and ammonium) also resulted in voltage transients, internal acidification being correlated with a hyperpolarization, and internal alkalinization with a depolarization. Also changes of external pH caused voltage responses, alkalinization causing a hyperpolarization, acidification a depolarization. Methazolamide, an inhibitor of carbonic anhydrase, as well as stilbenes (SITS or DIDS) caused a reduction of the voltage response to HCO −3 and pH. Their effects were additive. It is suggested that corneal endothelial cells possess one or two electrogenic transporters for HCO −3 or related species, one of which is inhibitable by stilbenes.

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Authors and Affiliations

  1. Institut für Klinische Physiologie, Klinikum Steglitz, Freie Universität Berlin, D-1000, Berlin 45, West Germany

    T. J. Jentsch, M. Koch & M. Wiederholt

  2. Augenklinik, Klinikum Charlottenburg, Freie Universität Berlin, D-1000, Berlin 19, West Germany

    H. Bleckmann

Authors
  1. T. J. Jentsch
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  2. M. Koch
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  3. H. Bleckmann
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  4. M. Wiederholt
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Jentsch, T.J., Koch, M., Bleckmann, H. et al. Effect of bicarbonate, pH, methazolamide and stibenes on the intracellular potentials of cultured bovine corneal endothelial cells. J. Membrain Biol. 78, 103–117 (1984). https://doi.org/10.1007/BF01869198

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  • DOI: https://doi.org/10.1007/BF01869198

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