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The functional significance of the evolutionary divergence between the tryptophan operons ofEscherichia coli andSalmonella typhimurium

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Summary

Hybrid strains ofEscherichia coli andSalmonella typhimurium were constructed in which intergeneric complementation was required for tryptophan biosynthesis. The functional aspects of in vivo tryptophan biosynthesis in these hybrid strains were compared with similar strains ofE. coli. There was little indication that the hybrid strains were deficient in tryptophan biosynthesis as a result of their hybrid nature, although the tryptophan operons of the two species differ by approximately one thousand mutations. It is tentatively concluded that this genetic divergence has not detectably altered the functional properties of the operon and is likely to be the result of accumulation of neutral mutations.

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Abbreviations

PRA:

N-(5′ phophoribosyl)anthranilic acid

CdRP:

1-(o-carboxyphenylamino)-1-deoxyribulose phosphate

InGP:

indole-3-glycerol phosphate

InG(P):

InGP or its dephosphorylated form

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  1. Medical Research Council Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, CB2 2QH, Cambridge, UK

    Thomas E. Creighton

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Creighton, T.E. The functional significance of the evolutionary divergence between the tryptophan operons ofEscherichia coli andSalmonella typhimurium . J Mol Evol 4, 121–137 (1974). https://doi.org/10.1007/BF01732018

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  • DOI: https://doi.org/10.1007/BF01732018

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