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Induction of hepatic CYP1A activity as a biomarker for environmental exposure to Aroclor® 1254 in feral rodents

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Abstract

Specimens of the feral mouse species Reithrodontomys fulvescens trapped from a polychlorinated biphenyl (PCB)-contaminated field location had hepatic ethoxy-resorufin (ETR) O-dealkylase activities and immunoreactive CYP1A protein contents which were two- to threefold higher than those measured in animals of the same species and sex collected from non PCB-contaminated reference sites. Specimens with hepatic ETR O-dealkylase activities differing by as little as 50% could readily be assigned as originating from the PCB or reference sites by the use of a specific chemical inhibitor of cytochrome P450IA (CYP1A). The relative levels of ETR O-dealkylase activity in R. fulvescens significantly correlated with hepatic PCB burdens (r=0.819, P<0.01). When the magnitudes of the induced ETR O-dealkylase activities corresponding to given hepatic PCB burdens were compared between the feral animals, F344/NCr rats (Rattus norvegicus) or B6C3F1 mice (Mus musculus) exposed in the laboratory to dietary Aroclor® 1254, the order of sensitivity to the inducing effects of PCBs were F344/NCr rat>B6C3F1 mouse>R. fulvescens.

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Authors and Affiliations

  1. Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, Maryland, USA

    Ronald A. Lubet, Raymond W. Nims & Lisa E. Beebe

  2. Chemical Synthesis and Analysis Laboratory, PRI-DynCorp, Frederick Cancer Research and Development Center, 21702-1201, Frederick, Maryland, USA

    Stephen D. Fox & Haleem J. Issaq

  3. Department of Zoology, Oklahoma State University, 74708, Stillwater, Oklahoma, USA

    Karen McBee

Authors
  1. Ronald A. Lubet
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  2. Raymond W. Nims
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  3. Lisa E. Beebe
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  4. Stephen D. Fox
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  5. Haleem J. Issaq
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  6. Karen McBee
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Lubet, R.A., Nims, R.W., Beebe, L.E. et al. Induction of hepatic CYP1A activity as a biomarker for environmental exposure to Aroclor® 1254 in feral rodents. Arch. Environ. Contam. Toxicol. 22, 339–344 (1992). https://doi.org/10.1007/BF00212096

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  • DOI: https://doi.org/10.1007/BF00212096

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