Abstract
The 96-h toxicity of four organophosphates (thiometon, disulfoton, malathion, and demeton-S-methyl, the oxygen analogue of thiometon) in the freshwater bivalve mollusc Dreissena polymorpha was tested using different nominal concentrations ranging between 6 and 50 mg/L. No mortalities were observed in mussels exposed to malathion and demeton-S-methyl (26 mg/L and 6 mg/L, respectively), and at the lowest concentrations of thiometon and disulfoton (6 and 10 mg/L, respectively). At higher thiometon and disulfoton concentrations, mortalities occurred. At the highest concentrations of 50 mg thiometon/L and 30 mg disulfoton/L, mussel mortalities of 88 and 93%, respectively, were determined. Organophosphate concentrations of up to a factor 10 times higher than in the ambient water were found in exposed mussels, irrespective of whether they were alive or dead. The search for organophosphate metabolites via GC/MS analysis of mussel tissue extracts was negative, suggesting lacking or low oxidative activation of the insecticides used. The mollusc is highly resistant to toxic effects of organophosphate insecticides and their biological active oxygen analogues.
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References
Arnold H, Braunbeck T (1992) Disulfoton as a major toxicant in the river Rhine chemical spill at Basle in 1986: Acute and chronic studies with eel and rainbow trout. EIFAC/XVII/Symp E9
ASTM (1990) Standard guide for conducting acute toxicity tests with fish, macroinvertebrates, and amphibians. In: Annual book of ASTM standards, Volume 11.04. Philadelphia, PA, pp 360–379
Capel PD, Giger W, Reichert P, Wanner O (1988) Accidental input of pesticides into the Rhine river. Environ Sci Technol 22:992–996
De Bruijn J, Hermens J (1991) Uptake and elimination kinetics of organophosphrous pesticides in the guppy (Poecilla reticulata): Correlations with the octanol/water partition coefficient. Environ Toxicol Chem 10:791–804
DFG (1982) Organochlor-und Organophosphor-Pestizide. In: Rückstandsanalytik von Pflanzenschutzmittel. Deutsche Forschungs-gemeinschaft. Weinheim, Deerfield Beach Florida, Basel, pp 1–13
EAWAG (1987) Verhalten der Chemikalien im Rhein, biologischer Zustand und Wiederbelebung des Rheins nach dem Brand in Schweizerhalle. Swiss Federal Institute for Water Resources and Water Pollution Control (EAWAG). Dübendorf, Switzerland, Project 4727
Ferrario JB, DeLeon IR, Peuler EA (1994) Bioaccumulation of chemical markers as a means for the field detection and verification of organophosphorus warfare agents. Environ Sci Technol 28:1893–1897
Frank R, Braun HE, Chapman N, Burchart C (1991) Degradation of parent compounds of nine organophosphorus insecticides in Ontario surface and ground waters under controlled conditions. Bull Environ Contam Toxicol 47:374–380
Gallo MA, Lawryk NJ (1991) Organic phosphorus pesticides. In: Hayes WJ, Hayes ERL (eds) Handbook of pesticide toxicology. Academic Press, Inc., San Diego, CA, pp 917–1090
Güttinger H, Stumm W (1992) An analysis of the Rhine pollution caused by the Sandoz chemical accident, 1986. Interdisciplin Sci Rev 17:127–136
Hall RJ, Kolbe E (1980) Bioconcentration of organophosphorus pesticides to hazardous levels by amphibians. J Toxicol Environ Health 6:853–860
Heyer CB, Kater SB, Karlsson UL (1973) Neuromuscular systems in molluscs. Am Zool 13:247–270
Johnson MK (1975) The delayed neuropathy caused by some organophosphorus esteres: Mechanisms and challenge. Crc Crit Rev Toxicol 3:289–316
Lotti M (1992) The pathogenesis of organophosphate polyneuropathy. Crc Crit Rev Toxicol 21:465–487
Mahendru VK, Agarwal RA (1983) Phorate and mexacarbate induced changes in enzymes of the snail, Lymnaea acuminata. Arch Environ Contam Toxicol 12:77–82
Mayer FL, Ellersieck MR (1986) Manual of acute toxicity: Interpretation and data base for 410 chemicals and 66 species of freshwater animals. Washington, DC
McLeese DW, Zitko V, Sergeant DB (1979) Uptake and excretion of fenitrothion by clams and mussels. Bull Environ Contam Toxicol 22:800–806
Mercer AL, McGregor DD (1982) Neural regulation and pharmacology of the gut of Chione stutchburyi, a bivalve mollusc. Comp Biochem Physiol 73C:243–251
Miller CW, Zuckerman BM, Charig AJ (1966) Water translocation of diazinon-C14 and parathion-S35 of a model cranberry bog and subsequent occurrence in fish and mussels. Trans Amer Fish Soc 95:345–349
Morton B (1969) Studies on the biology of Dreissena polymorpha Pall. I. General anatomy and morphology. Proc Malac Soc Lond 38:301–321
Nichols SJ (1992) Maintenance of the zebra mussel (Dreissena polymorpha) under laboratory conditions. In: Nalepa TF, Nalepa DS (eds) Zebra mussels: Biology, impacts, and control. Boca Raton, Florida, pp 733–747
Payne JF, Fancey LL, Rahimtula AD, Porter EL (1987) Review and perspective on the use of mixed-function oxygenase enzymes in biological monitoring. Comp Biochem Physiol 86C:233–245
Prozorovskii VB, Skopichev VG (1993) Morphological changes in mouse and rat erythrocytes upon exposure to cholinesterase organophosphorus inhibitors. Bull Exp Biol Med 115:479–481
Rosza K (1984) The pharmacology of molluscan neurons. Prog Neurobiol 23:79–150
Singh AK, Zeleznikar RJ, Drewes LR (1985) Analysis of soman and sarin in blood utilizing a sensitive gas chromatography-mass spectrometry method. J Chromatogr 324:163–172
Suter W (1982) Vergleichende Nahrungsökologie von überwinternden Tauchenten (Bucephala, Aythya) und Bläßhuhn (Fulica atra) am Untersee-Ende/Hochrhein (Bodensee). Ornitholog Beobachter 79:225–254
Takase I, Oyama H (1985) Uptake and bioconcentration of disulfoton and its oxidation compounds in carp, Cyprinus carpio. L. J Pestic Sci 10:47–53
Villar D, Gonzalez M, Gualda MJ, Schaeffer DJ (1994) Effects of organophosphorus insecticides on Duglesia tigrina: Cholinesterase activity and head regeneration. Bull Environ Contam Toxicol 52:319–324
Wanner O, Egli T, Fleischmann T, Lanz K, Reichert P, Schwarzenbach RP (1989) Abbau von Thiometon und Disulfoton im Wasser. Environ Sci Technol Appl 32:1232–1342
Ward TR, Ferris DJ, Tilson HA, Mundy WR (1993) Correlation of the anticholinesterase activity of a series of organophosphates with their ability to compete with agonist binding to muscarinic receptors. Toxicol Appl Pharmacol 122:300–307
Willems JL, De Bisschop HC, Verstraete AG, Declerck C, Christiaens Y, Vanscheeuwyck P, Buylaert WA, Vogelaers D, Colardyn F (1993) Cholinesterase reactivation in organophosphorus poisoned patients depends on the plasma concentrations of the oxime pralidoxime methylsulphate and of the organophosphate. Arch Toxicol 67:79–84
Wisson M, Van Hoek C, Sauer HH (1976) Thiometon. In: Analytical methods for pesticides and plant growth regulators, Vol. VIII. Academic Press, NY, pp 239–248
Yashiki M, Kojima T, Ohtani M, Chikasue F, Miyazaki T (1990) Determination of disulfoton and its metabolites in the body fluids of a Di-syston® intoxication case. Forensic Sci Int 48:145–154
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Dauberschmidt, C., Dietrich, D.R. & Schlatter, C. Toxicity of organophosphorus insecticides in the zebra mussel, Dreissena polymorpha P.. Arch. Environ. Contam. Toxicol. 30, 373–378 (1996). https://doi.org/10.1007/BF00212296
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DOI: https://doi.org/10.1007/BF00212296