Skip to main content
SpringerLink
Log in

Thirteen loci physically assigned to sheep Chromosome 2 by cell hybrid analysis and in situ hybridization

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

Sheep x hamster cell hybrids containing sheep metacentric Chromosome (Chr) 2 were produced by fusing blood leukocytes from normal sheep with hamster auxotrophic Ade F-minus mutants. Cell clones that were isocitrate dehydrogenase 1 (IDH1) positive were cytogenetically characterized, confirming that they contained sheep Chr 2. The following loci were newly assigned by Southern hybridization to sheep Chr 2: lipoprotein lipase (LPL), glycoprotein-4-beta galactosyltransferase 2 (GGTB2), neurofilament light polypeptide (68 kDa; NEFL), surfactant-associated protein 2 (SFTP2), lymphocyte-specific protein tyrosine kinase (LCK), and nebulin (NEB). These new assignments and the in situ localization of gelsolin (GSN) to sheep Chr 2pter-p24 are consistent with the predicted homology of cattle Chr 8 (U18) with sheep Chr 2p, and of cattle Chr 2 (U17) with sheep 2q. In addition, the assignment by cell hybrid analysis of loci previously mapped to Chr 2 in sheep, viz., cholinergic receptor, nicotinic, delta polypeptide (CHRND), collagen type III alpha 1 (COL3A1), fibronectin 1 (FN1), isocitrate dehydrogenase (IDH1), and villin 1 (VIL1), confirmed the localization of sheep syntenic group U11 to this chromosome. By nutritional selection and complementation of the hamster auxotrophic Ade F mutation, the multifunctional enzyme locus phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase)/IMP cyclohydrolase (inosinicase) (provisionally given the symbol PRACFT) has also been newly assigned to sheep Chr 2. This report significantly extends the number of loci physically mapped to sheep Chr 2 and confirms its close homology with cattle Chrs 2 and 8.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ansari, H.A., Pearce, P.D., Maher, D.W., Malcolm, A.A., Broad, T.E. (1993). Resolving ambiguities in the karyotype of the domestic sheep (Ovis aries). Chromosoma 102, 340–347.

    Google Scholar 

  • Ansari, H.A., Pearce, P.D., Maher, D.W., Malcolm, A.A., Wood, N.J., Phua, S.H., Broad, T.E. (1994). Regional mapping of loci from human chromosome 2q to sheep chromosome 2q. Genomics 20, 122–124.

    Google Scholar 

  • Barendse, W., Armitage S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994). A genetic linkage map of the bovine genome. Nature Genet. 6, 227–235.

    Google Scholar 

  • Beever, J.E., Da, Y., Ron, M., Lewin H.A. (1994). A genetic map of nine loci on bovine Chromosome 2. Mamm. Genome 5, 542–545.

    Google Scholar 

  • Blattman, A.N., Beh, K.J. (1992). Dinucleotide repeat polymorphism within the ovine major histocompatibility complex. Anim. Genet. 23, 392.

    Google Scholar 

  • Brotherton, D., Chu, M-L, Humphries, S.E., Henney, A.M. (1989). An NcoI RFLP associated with the gene encoding the α-3 chain of human type VI collagen (COL6A3). Nucleic Acids Res. 17, 1274.

    Google Scholar 

  • Bunch, T.D., Foote, W.C., Spillett, J.J. (1976). Translocations of acrocentric chromosomes and their implications in the evolution of sheep (Ovis). Cytogenet. Cell Genet. 17, 122–136.

    Google Scholar 

  • Burkin, D.J., Morse, H.G., Broad, T.E., Pearce, P.D., Ansari, H.A., Lewis, P.E., Jones, C., (1993). Mapping the sheep genome: production of characterized sheep x hamster cell hybrids. Genomics 16, 466–472.

    Google Scholar 

  • Dietz, A.B., Womack, J.E., Swarbrick, P.A., Crawford, A.M. (1993). Assignment of five polymorphic ovine microsatellites to bovine syntenic groups. Anim. Genet. 24, 433–436.

    Google Scholar 

  • Droogmans, L., Cludts, I., Cleuter, Y., Kettmann, R., Burny, A. (1992). Nucleotide sequence of bovine interleukin-6-cDNA. J. DNA Seq. Mapping 2, 411–414.

    Google Scholar 

  • Echard, G., Broad, T.E., Hill, D, Pearce, P. (1994). Present status of the bovine gene map (Ovis aries): comparison with the bovine map (Bos taurus). Mamm. Genome 5, 324–332.

    Google Scholar 

  • Fries, R., Eggen, A., Womack, J.E. (1993). The bovine genome map. Mamm. Genome 4, 405–428.

    Google Scholar 

  • Glasser, S.W., Korfhagen, T.R., Weaver, T.E., Clark J.C., Pilot-Matias, T., Meuth, J., Fox, J.L., Whitsett, J.A. (1988). cDNA, deduced polypeptide structure and chromosome assignment of human pulmonary surfactant proteolipid, SPL(pVal). J. Biol. Chem. 263, 9–12.

    Google Scholar 

  • Hay, R.E., Woods, W.D., Church, R.L., Petrash, J.M. (1987). cDNA clones encoding bovine γ-crystallins. Biochem. Biophys. Res. Commun. 146, 332–338.

    Google Scholar 

  • Hayes, H.C., Popescu, P., Dutrillaux, B. (1993). Comparative gene mapping of lactoperoxidase, retinoblastoma, and α-lactalbumin genes in cattle, sheep and goats. Mamm. Genome 4, 593–597.

    Google Scholar 

  • Heriz, A., Arruga, M.V., Montagudo L.V., Tejedor, M.T., Pitel, F., Echard, G. (1994). Assignment of the transition protein 1 (TPN1) gene to the U17 bovine synteny group by PCR. Mamm. Genome 5, 742.

    Google Scholar 

  • Iannuzzi, L., Gallagher, D.S., Ryan, A.M., Di Meo, G.P., Womack, J.E. (1993a). Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization. Cytogenet. Cell Genet. 62, 224–227.

    Google Scholar 

  • Iannuzzi, L., Di Meo, G.P., Gallagher, D.S., Ryan, A.M., Ferrara, L., Womack, J.E. (1993b). Chromosome localization of omega and trophoblast interferon genes in goat and sheep by fluorescent in situ hybridization. J. Hered. 84, 301–304.

    Google Scholar 

  • ISCNDA, 1989 (1990). International system for cytogenetic nomenclature of domestic animals. D. DiBerardino, H. Hayes, R. Fries, S. Long, eds. Cytogenet Cell Genet. 53, 65–79.

  • Jones, C., Morse, H.G., Patterson, D. (1986). Assignment of the gene complementing the auxotrophic marker Ade-F to human Chromosome 2. Am. J. Hum. Genet. 39, A158 (Abstract).

  • Kornblihtt, A.R., Vibe-Pedersen, K., Baralle, F.E. (1983). Isolation and characterization of cDNA clones for human and bovine fibronectin. Proc. Natl. Acad. Sci. USA 80, 3218–3222.

    Google Scholar 

  • Kubo, T., Noda, M., Takai, T., Tanabe, T., Kayano, T., Shimizu, S., Tanaka, K., Takahashi, H., Hirose, T., Inayama, S., Kikuno, R., Miyata, T., Numa, S. (1985). Primary structure of the δ subunit precursor of calf muscle acetylcholine receptor deduced from cDNA sequence. Eur. J. Biochem. 149, 5–13.

    Google Scholar 

  • Kwiatkowski, D.J., Stossel, T.P., Orkin, S.H., Mole, J.E., Colten, H.R., Yin, H.L. (1986). Plasma and cytoplasmic gelsolin are encoded by a single gene and contain a duplicated actin-binding domain. Nature 323, 455–458.

    Google Scholar 

  • Logan, C., Joyner, A.L. (1989). An SstI RFLP for the human homeo box-containing gene EN2. Nucleic Acids Res. 17, 2878.

    Google Scholar 

  • Long, S.E., (1990). Chromosomes of sheep and goats. In: Domestic Animal Cytogenetics, R.A. McFeely, ed. Adv. Vet. Sci. Compar. Med. 34, 109–129.

  • Lopez, L.C., Frazier, M.L., Su, C-J., Kumar, A., Saunders, G.F. (1983). Mammalian pancreatic pre-proglucagon contains three glucagon-related peptides. Proc. Natl. Acad. Sci., USA 80, 5485–5489.

    Google Scholar 

  • Malo, D., Schurr, E., Dorfman, J., Canfield, V., Levenson, R., Gros, P. (1991). Three brain sodium channel α-subunit genes are clustered on the proximal segment of mouse chromosome 2. Genomics 10, 666–672.

    Google Scholar 

  • Marth, J.D., Disteche, C., Pravtcheva, D., Ruddle, F., Krebbs, E.G., Perlmutter, R.M. (1986). Localization of the lymphocyte-specific protein tyrosine kinase gene (lck) at a site of frequent chromosome abnormalities in human lymphomas. Proc. Natl. Acad. Sci. USA 83, 7400–7404.

    Google Scholar 

  • McAlpine, P.J., Shows, T.B., Boucheix, C., Pericak-Vance, M.A., Anderson, W.A. (1993). The 1992 catalog of mapped genes and report of the nomenclature committee. Genome Prior. Rep. 1, 11–142.

    Google Scholar 

  • Meera Khan, P. (1971). Enzyme electrophoresis on cellulose acetate gel: zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybrids. Arch. Biochem. Biophys. 145, 470–483.

    Google Scholar 

  • Miskulin, M., Dalgleish, R., Kluve-Beckerman, B., Rennard, S.I., Tolstoshev, P., Brantly, M., Crystal, R.G. (1986). Human type III collagen gene expression is coordinately modulated with the type I collagen genes during fibroblast growth. Biochemistry 25, 1408–1413.

    Google Scholar 

  • Neibergs, H.L., Gallagher, D.S., Georges, M., Sargeant, L.S., Womack, J.E. (1993). Physical mapping of inhibin beta A in domestic cattle. Mamm. Genome 4, 328–332.

    Google Scholar 

  • Ni, L., Guan, K., Zalkin, H., Dixon J.E. (1991). De novo purine nucleotide biosynthesis: cloning, sequencing and expression of a chicken PurH cDNA encoding 5-aminoimidazole 4-carboxamide-ribonucleotide transformylase-IMP cyclohydrolase. Gene 106, 197–215.

    Google Scholar 

  • O'Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.

    Google Scholar 

  • Patterson, D. (1985). De novo purine and pyrimidine biosynthesis. In: Molecular Cell Genetics, M. Gottesman, ed. New York: John Wiley and Sons Inc. pp. 286–309.

    Google Scholar 

  • Pearce, P.D., Ansari, H.A., Maher, D.W., Broad, T.E. (1995). Five regional localizations to the sheep genome: first assignments to chromosomes 5 and 12. Anim Genet. 26, 171–176.

    Google Scholar 

  • Penty, J.M., Montgomery, G.W. (1991). Genetic linkage between α-inhibin and fibronectin 1 in sheep. Proc. Australas. Gene Mapping Mol. Genet. Symp. Dunedin, Aug 26–30, abstr. 32.

  • Pitel, F., Lantier, I., Taber-Aoul, K., Saidi-Mehtar, N., Elsen, J-M, Lantier, F., Gellin, J. (1994). TNP1 gene: a sheep polymorphic marker for a conserved region among mammals. Mamm. Genome 5, 390–392.

    Google Scholar 

  • Pringault, E., Arpin, M., Garcia, A., Finidori, J., Louvard, D. (1986). A human villin cDNA clone to investigate the differentiation of intestinal and kidney cells in vivo and in culture. EMBO J. 5, 3119–3124.

    Google Scholar 

  • Shaper, N.L., Shaper, J.H., Meuth, J.L., Fox, J.L., Chang, H., Kirsch, I.R., Hollis, G.F. (1986). Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library. Proc. Natl. Acad. Sci. USA 83, 1573–1577.

    Google Scholar 

  • Tabet-Aoul, K., Pitel, F., Lantier, I., Gros, P., Saidi-Mehtar, N., Lantier, F., (1992). Mapping of the ovine genome by somatic hybrid cell lines: the IDH1, FN1, VIL1 and CHRNG syntenic group is conserved in sheep. Anim. Genet. 23 (Suppl. 1), 96.

    Google Scholar 

  • Takai, T., Noda, M., Furatani, Y., Takahashi, H., Notake, M., Shimizu, S., Kayano, T., Tanabe, T., Tanaka, K., Hirose, T., Inayama, S., Numa, S. (1984). Primary structure of γ subunit precursor of calf-muscle acetylcholine receptor deduced from the cDNA sequence. Eur. J. Biochem. 143, 109–115.

    Google Scholar 

  • Tolan, D.R., Niclas, J., Bruce, B.D., Lebo, R.V. (1987). Evolutionary implications of the human aldolase-A,-B,-C, and pseudogene chromosome locations. Am. J. Hum. Genet. 41, 907–924.

    Google Scholar 

  • Van Someren, H., Van Henegouwen, N.B., Los, W., Wurzer-Figurelli, E., Doppert, B., Vervloet, M., Meera Khan, P. (1974). Enzyme electrophoresis on cellulose acetate gel. II. Zymogram patterns in man-Chinese hamster somatic cell hybrids. Humangenetik 25, 189–201.

    Google Scholar 

  • Wijnen, L.M.M., Grzeschik, K.-H., Pearson, P.L., Meera Khan, P. (1977). The human PGM2 and its chromosomal localization in man-mouse hybrids. Hum. Genet. 37, 271–278.

    Google Scholar 

  • Wion, K.L., Kirschgessner, T.G., Lusis, A.J., Schotz, M.C., Lawn, R.M. (1987). Human lipoprotein lipase complementary DNA sequence. Science 235, 1638–1641.

    Google Scholar 

  • Womack, J.E., Moll, Y.D. (1986). Gene map of the cow: conservation of linkage with mouse and man. J. Hered. 77, 2–7.

    Google Scholar 

  • Yamauchi, M., Auysawa, D., Shimizu, K., Seno, T., Matsuhashi, M. (1989). Two types of mouse FM3A cell mutants deficient in 5-amino-imidazole-4-carboxamide ribonucleotide transformylase and their transformants isolated by human chromosome-mediated gene transfer. Somatic Cell Mol. Genet. 15, 39–48.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. AgResearch Grasslands Research Centre, Palmerston North, New Zealand

    T. E. Broad, P. E. Lewis, A. J. Gleeson, P. D. Pearce, D. W. Maher & H. A. Ansari

  2. Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, Colorado, USA

    D. J. Burkin & C. Jones

  3. Laboratory of Viral Carcinogenesis, NIH-National Cancer Institute, 21701, Frederick, Maryland, USA

    M. A. Carpenter

  4. Department of Biochemistry, Biophysics & Genetics, University of Colorado Health Sciences Center, 80206, Denver, Colorado, USA

    C. Jones

Authors
  1. T. E. Broad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. E. Lewis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. J. Burkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. J. Gleeson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. A. Carpenter
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. P. D. Pearce
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. D. W. Maher
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. H. A. Ansari
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Broad, T.E., Lewis, P.E., Burkin, D.J. et al. Thirteen loci physically assigned to sheep Chromosome 2 by cell hybrid analysis and in situ hybridization. Mammalian Genome 6, 862–866 (1995). https://doi.org/10.1007/BF00292436

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00292436

Keywords

Search

Navigation