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Mechanism of bradykinin-induced cyclic GMP accumulation in bovine tracheal smooth muscle

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Abstract

Bradykinin (10−8-10−5M) caused a concentration-dependent increase in cyclic GMP (cGMP) production in bovine tracheal smooth muscle in the absence of epithelium. The effect was calcium-dependent and was inhibited by pyrogallol (10 μM) and methylene blue (10 μM). The inhibition of pyrogallol was reversed by superoxide dismutase (100 Usnowml). Nitric oxide (NO) synthase inhibitors, N G-methyl-l-arginine (10–100 μM) and N G-nitro-l-arginine (10–100 μM) reduced cGMP accumulation induced by bradykinin in a concentration-dependent fashion, and the inhibition was reversed by l-arginine. Immunohistochemistry with a specific antibody against neuronal NO synthase from rat cerebellum showed positive staining localized in some nerve fibers. Bradykinin-induced cGMP accumulation appears to be related to the release of NO, part of which is probably synthesized in nonadrenergic noncholinergic nerve in bovine trachea.

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Authors and Affiliations

  1. Signal Transduction Group, Abbott Laboratories, 60064, Abbott Park, Illinois

    H. Sheng, K. Ishii, U. Förstermann & F. Murad

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  1. H. Sheng
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  2. K. Ishii
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  3. U. Förstermann
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  4. F. Murad
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Offprint requests to: Dr. Hong Sheng

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Sheng, H., Ishii, K., Förstermann, U. et al. Mechanism of bradykinin-induced cyclic GMP accumulation in bovine tracheal smooth muscle. Lung 173, 373–383 (1995). https://doi.org/10.1007/BF00172144

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