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Expression of HGF and TGF-β1 mRNA after partial hepatectomy in rats with liver cirrhosis

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Abstract

Hepatocyte growth factor (HGF) is a potent mitogen for the maturation of hepatocytes in vitro which plays a role in liver regeneration in vivo. In addition, transforming growth factor-β1 (TGF-β1) is also a potent regulator of liver regeneration. In attempting to clarify the mechanisms related to liver regeneration after partial hepatectomy, we investigated the expression of HGF and TGF-β1 in rats with liver cirrhosis (LC). A rat model of LC was prepared using carbon tetrachloride (CCl4). The expression of HGF mRNA in both the LC and control groups showed a similar time-course with the highest expression seen at 18 h after a 70% hepatectomy. The expression of TGF-β1 mRNA peaked at 18 h after partial hepatectomy in the LC group and at 48 h in the control group. The 5-bromo-2'-deoxyuridine (BrdU) labeling index for the LC group at 24, 48, and 72 h after partial hepatectomy was 9.2%, 5.9%, and 1.8%, while for the control group it was 7.0%, 11.7%, and 6.8%, respectively. The BrdU labeling index in the LC group was thus suppressed earlier than that in the control group. We therefore postulate that regeneration of the remnant liver in the presence of LC accelerates immediately after partial hepatectomy, but the extent of regeneration is insufficient because of an early cessation due to an early expression of TGF-β1.

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Authors and Affiliations

  1. First Department of Surgery, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan

    Shinji Mitsue, Masahiro Hamanoue, Gen Tanabe, Yoshito Ogura, Shinrou Yoshidome & Takashi Aikou

  2. Biomedical Research Center, Osaka University Medical School, 2-2 Yamadaoka, 565, Suita, Osaka, Japan

    Toshikazu Nakamura

Authors
  1. Shinji Mitsue
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  2. Masahiro Hamanoue
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  3. Gen Tanabe
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  4. Yoshito Ogura
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  5. Shinrou Yoshidome
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  6. Takashi Aikou
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  7. Toshikazu Nakamura
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Mitsue, S., Hamanoue, M., Tanabe, G. et al. Expression of HGF and TGF-β1 mRNA after partial hepatectomy in rats with liver cirrhosis. Surg Today 25, 237–243 (1995). https://doi.org/10.1007/BF00311534

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  • DOI: https://doi.org/10.1007/BF00311534

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