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Neuronal differentiation of human neuroblastoma SH-SY5Y cells by gangliosides

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Abstract

Exogenous administration of gangliosides induced neuronal differentiation with prominent neuritogenesis in human neuroblastoma SH-SY5Y cells in vitro. Neuritogenesis was characterized by ruffling of the cell membrane, the development of lamellipodia and filopodia, and the subsequent elongation and branching of the neurites ultrastructurally. Both axons and neurites were identified. Increased numbers of cell organelles in the neurites and cell bodies were noted. Nonsynaptic contacts and gap junctions formed between neurites or between each neurite and cell body. These findings could be implicated in histopathologic changes from neuroblastoma to ganglioneuroblastoma.

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Authors and Affiliations

  1. Department of Pathology, Chonnam National University Medical School and Medical Center, 8 Hakdong, Dongku, 501-190, Kwangju, Korea

    Min-Cheol Lee, Jong-Soon Kim, Ji-Shin Lee, Kyung-Soo Kim, Jae-Hyuk Lee & Jong-Hee Nam

  2. Department of Rehabilitation Medicine, Chonnam National University Medical School and Medical Center, Kwangju, Korea

    Bong-Woon Kim & Sung-Man Rowe

  3. Department of Medicine, University of British Columbia, Vancouver, BC, Canada

    Seung-U. Kim

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  1. Min-Cheol Lee
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  2. Bong-Woon Kim
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  3. Jong-Soon Kim
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  4. Ji-Shin Lee
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  9. Seung-U. Kim
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Lee, MC., Kim, BW., Kim, JS. et al. Neuronal differentiation of human neuroblastoma SH-SY5Y cells by gangliosides. Brain Tumor Pathol 14, 5–11 (1997). https://doi.org/10.1007/BF02478862

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  • DOI: https://doi.org/10.1007/BF02478862

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