Skip to main content
Log in

A study of uricase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen

  • Original Papers
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A uricase biosensor was constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize uricase on a glassy carbon electrode modified with Nafion and methyl viologen (MV). A linear response of uric acid in serum was observed in a range of 1.0·10−3 to 5.0·10−6 mol/1 and the response time is 25 s. This biosensor has the advantage of high sensitivity, fast response as compared with previous sensors and low interferences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. G. G. Guilbault,Analytical Uses of Immobilized Enzymes, Marcel Dekker, New York, 1984.

    Google Scholar 

  2. M. Nanjo, G. G. Guilbault,Anal. Chem. 1974,46, 1769.

    PubMed  Google Scholar 

  3. W. Dritschilo, M. Weibel,Biochem. Med. 1974,11, 242.

    PubMed  Google Scholar 

  4. T. Kawashima, A. Arima, N. Hatakeyama, N. Tominaga, M. Ando,Nippon Kagaku Kaishi,1980, 1542.

  5. Jin Litong, Jin Ping, Ye Jiannong, Fang Yuzhi,Talanta 1992,39, 145.

    Google Scholar 

  6. Shanghai Institute of Medical Analysis,Clinical Biochemical Analysis, Shanghai Science and Technology Publishing House, 1979.

  7. Jin Litong, Liu Haiying, Fang Yuzhi,J. East China Normal Univ. (Special Edition in Chemistry) 1991,Sept., 50.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, L., Ye, J., Tong, W. et al. A study of uricase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen. Mikrochim Acta 112, 71–75 (1993). https://doi.org/10.1007/BF01243323

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01243323

Key words

Navigation