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Isozyme diversity, RFLP of the rDNA and phylogenetic affinities among cultivated Lima beans,Phaseolus lunatus (Fabaceae)

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Abstract

Genetic variation inPhaseolus lunatus (Lima bean) was investigated at isozyme and DNA levels. Sixty cultivated accessions, including representatives of the Mesoamerican and Andean gene pools and intermediate types, were analyzed for variability at 17 isozyme loci. Some accessions were also examined for restriction fragment length polymorphism (RFLP) at the rDNA level. These data were used to construct two dendrograms showing clear separation in two distinct groups corresponding to each of the gene pools and an intermediate one probably representing a transitional group.

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References

  • Becerra Velasquez, V., Gepts, P., 1994: RFLP diversity of common bean (Phaseolus vulgaris) in its centres of origin. — Genome37: 256–263.

    Google Scholar 

  • Dellaporta, S. L., Wood, J., Hicks, J. B., 1983: A plant DNA minipreparation: version II. — Pl. Molec. Biol. Reporter.1: 19–21.

    Google Scholar 

  • Freyre, R., Rios, R., Guzman, L., Debouck, D. G., Gepts, P., 1996: Ecogeographic distribution ofPhaseolus spp. (Fabaceae) in Bolivia. — Econ. Bot.50: 195–215.

    Google Scholar 

  • Garvin, D. F., Roose, M. L., Waines, J. G., 1989: Isozyme genetics and linkage in tepary bean,Phaseolus acutifolius A. Gray. — J. Heredity80: 373–376.

    Google Scholar 

  • —, 1990: Aconitase variation in tepary bean suggests a common origin for domesticated landraces. — Report (Annual) Bean Improv. Coop.33: 132–133.

    Google Scholar 

  • Gepts, P., Osborn, T. C., Rashka, K., Bliss, F. A., 1986: Phaseolin protein variability in wild forms and landraces of common bean (Phaseolus vulgaris): evidence for multiple centers of domestication. — Econ. Bot.40: 451–468.

    Google Scholar 

  • Gerlach, W. L., Bedbrook, J. R., 1989: Cloning and characterization of ribosomal RNA genes from wheat and barley. — Nucl. Acids Res.7: 1869–1885.

    Google Scholar 

  • Gutierrez Salgado, A., Gepts, P., Debouck, D. G., 1995: Evidence for two gene pools of the Lima bean,Phaseolus lunatus L., in the Americas. — Genet. Res. Crop Evol.42: 15–28.

    Google Scholar 

  • Hussain, A., Bushuk, W., Ramirez, H., Roca, W., 1988: A practical guide for electrophoretic analysis of isoenzymes and proteins in cassava, field beans and forage legumes. — Working document no.40 Centro International de Agricultura Tropical (CIAT), Cali, Colombia.

  • Jaaska, V., 1996: Isoenzyme diversity and phylogenetic affinities among thePhaseolus beans (Fabaceae). — Pl. Syst. Evol.200: 233–252.

    Google Scholar 

  • Jacob, M., Zink, D., Nagl, W., 1995: RFLPs of the rDNA genes in the genusPhaseolus. — Genet. Res. Crop Evol.42: 97–106.

    Google Scholar 

  • Kaplan, L., Kaplan, N., 1988:Phaseolus in archaeology. — InGepts, P., (Ed.): Genetic resources ofPhaseolus beans, pp. 125–142. — Dordrecht: Kluwer.

    Google Scholar 

  • Koenig, R., Gepts, P., 1989: Allozyme diversity in wildPhaseolus vulgaris: further evidence for two major centers of diversity. — Theor. Appl. Genet.78: 809–817.

    Google Scholar 

  • Leitch, A. R., Heslop-Harrison, J. S., 1993: Ribosomal RNA gene expression and localization in cereals. — InSumner, A. T., Chandley, A. C., (Eds): Chromosomes today11, pp. 91–100. — London: Chapmann & Hall.

    Google Scholar 

  • Lioi, L., 1994: Morphotype relationships in Lima bean (Phaseolus lunatus L.) deduced from variation of the evolutionary marker phaseolin. — Genet. Res. Crop Evol.41: 81–85.

    Google Scholar 

  • —, 1996: Phaseolin diversity in wild Lima bean (Phaseolus lunatus L.) in American centres of origin. — Genet. Res. Crop Evol.43: 575–580.

    Google Scholar 

  • Mackie, W. W., 1943: Origin, dispersal and variability of the Lima bean,Phaseolus lunatus. — Hilgardia15: 1–24.

    Google Scholar 

  • Maggini, F., Tucci, G., Demartis, A., Gelati, M. T., Avanzi, S., 1992: Ribosomal RNA genes ofPhaseolus coccineus I. — Pl. Molec. Biol.18: 1073–1082.

    Google Scholar 

  • Maquet, A., Gutierrez, A., Debouck, D. G., 1990: Further biochemical evidence for the existence of two gene pools in Lima beans. — Report (Annual) Bean Improv. Coop.33: 128–129.

    Google Scholar 

  • —, 1996: Case studies on breeding systems and its consequences for germplasm conservation. 1. Isozyme diversity in wild Lima bean populations in central Costa Rica. — Genet. Res. Crop Evol.43: 309–318.

    Google Scholar 

  • Müeller-Starck, G., 1997: Isozymes. — InKarp, A., Isaac, P. G., Ingram, D. S., (Eds): Molecular tools for screening biodiversity: plants and animals. — London: Chapmann & Hall.

    Google Scholar 

  • Nei, M., 1978: Estimation of average heterozygosity and genetic diversity in subdivided population. — Genetics89: 583–590.

    Google Scholar 

  • Nienhuis, J., Tivang, J., Skroch, P., Dos Santos, J. B., 1995: Genetic relationships among cultivars and landraces of Lima bean (Phaseolus lunatus L.) as measured by RAPD markers. — J. Amer. Soc. Hort. Sci.120: 300–306.

    Google Scholar 

  • Panella, L., Gepts, P., 1992: Genetic relationships withinVigna unguiculata (L.)Walp. based on isozyme analyses. — Genet. Res. Crop Evol.39: 71–88.

    Google Scholar 

  • Schinkel, C., Gepts, P., 1989: Allozyme variability in the tepary bean,Phaseolus acutifolius A. Gray. — Pl. Breed.102: 182–195.

    Google Scholar 

  • Sparvoli, F., Daminati, M. G., Lioi, L., Bollini, R., 1996: In vivo endoproteolytically cleaved phaseolin is stable and accumulates in developingPhaseolus lunatus seeds. — Biochim. Biophys. Acta1292: 15–22.

    PubMed  Google Scholar 

  • Sprecher, S. L., 1988: New isozyme variants at two NADH diaphorase loci in dry beans: correlations to gene pools and commercial classes. — Report (Annual) Bean Improv. Coop.31: 92.

    Google Scholar 

  • Weeden, N. F., 1984: Linkage between the gene coding the small subunit of ribulose bisphosphate carboxylase and the gene coding malic enzyme inPhaseolus vulgaris. — Report (Annual) Bean Improv. Coop.27: 123–124.

    Google Scholar 

  • —, 1986: Genetic confirmation that the variation in the zymograms of 3 enzyme systems is produced by allelic polymorphism. — Report (Annual) Bean Improv. Coop.29: 117–118.

    Google Scholar 

  • Zink, D., Schumann, K., Nagl, W., 1994: Restriction fragment length polymorphysms of the phytohemagglutinin genes inPhaseolus andVigna (Leguminosae). — Pl. Syst. Evol.191: 131–146.

    Google Scholar 

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Correspondence to Lucia Lioi.

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Lioi, L., Lotti, C. & Galasso, I. Isozyme diversity, RFLP of the rDNA and phylogenetic affinities among cultivated Lima beans,Phaseolus lunatus (Fabaceae). Pl Syst Evol 213, 153–164 (1998). https://doi.org/10.1007/BF00985196

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  • DOI: https://doi.org/10.1007/BF00985196

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