Summary
Acute effects of occlusion of the middle cerebral artery on local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) were investigated quantitatively in separate groups of rats using (14C) iodoantipyrine (14C-IAP) or (14C) 2-deoxyglucose (14C-DG) respectively.
LCBF was significantly decreased in the ipsilateral cerebral cortices (to less than 45 ml/100 g/min or 30% of the control side) and the lateral part of the striatum (to 22 ml/100 g/min or 10% of the control side) which were supplied by the middle cerebral artery. No significant changes in LCBF were found in any other of the subcortical regions. In contrast to the unanimous decrease of LCBF in the ipsilateral cortices and the lateral striatum, complexed changes in LCGU were found in not only the cortex and striatum but also in many other subcortical regions which were closely related to the distribution of the mesencephalic dopamine neurons, such as globus pallidus, substantia nigra, subthalamic nucleus, nucleus accumbens, olfactory tubercle and lateral habenular nucleus. Relevance of this putative neurotransmitter and GABA on the glucose metabolism in ischemic brain is discussed.
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Abbreviations
- 2-DG:
-
2-deoxyglucose
- IAP:
-
iodoantipyrine
- LCGU:
-
local cerebral glucose utilization
- LCBF:
-
local cerebral blood flow
- MCA:
-
middle cerebral artery
- DA:
-
dopamine
- GABA:
-
gamma-aminobutyric acid
- NEN:
-
New England Nuclear Co.
References
Brown LL, Wolfson LI (1978) Apomorphine increases glucose utilization in the substantia nigra, subthalamic nucleus and corpus striatum of the rat. Brain Res 140: 188–193
Brown LL, Wolfson LI (1983) A dopamine-sensitive striatal efferent system mapped with (14C) deoxyglucose in the rat. Brain Res 261: 213–229
Diemer NH, Siemkowicz E (1980) Increased 2-deoxyglucose uptake in hippocampus, globus pallidus and substantia nigra after cerebral ischemia. Acta Neurol Scand 61: 56–63
Divac I, Diemer NH (1980) Prefrontal system in the rat visualized by means of labelled deoxyglucose — further evidence for functional heterogeneity of the striatum. J Comp Neurol 190: 1–13
Farber JL, Chien KR, Mittnacht S Jr (1981) The pathogenesis of irreversible cell injury in ischemia. Am J Pathol 102: 271–281
Ginsberg MD, Reivich M, Giandomenico A, Greenberg JH (1977) Local glucose utilization in acute focal cerebral ischemia: local dysmetabolism and diaschisis. Neurology (Minneap) 27: 1042–1048
Ginsberg MD, Reivich M (1979) Use of the 2-deoxyglucose method of local cerebral glucose utilization in the abnormal rat brain: evaluation of the lumped constant during ischemia. Acta Neurol Scand [Suppl] 60(72): 226–227
Gjedde A, Wienhard K, Heiss W-D, Kloster G, Diemer NH, Herholz K, Pawlik G (1985) Comparative regional analysis of 2-fluorodeoxyglucose and methylglucose uptake in brain of four stroke patients. With special reference to the regional estimation of the lumped constant. J Cereb Blood Flow Metab 5: 163–178
Hoedt-Rasmussen K, Skinhoj E, Paulson I, Ewald J, Bjerrum JK, Fahrenkrug A, Lassen NA (1967) Regional cerebral blood flow in acute apoplexy: The “luxury perfusion syndrome≓ of brain tissue. Arch Neurol 17: 271–281
Hossman KA, Schuier FJ (1980) Experimental brain infarcts in cats I. Pathophysiological observations. Stroke 11: 583–592
Kelly PAT, McCulloch J (1981) Cerebral blood flow and glucose utilization following dopaminergic and GABAergic manipulation. J Cereb Blood Flow Metab, vol 1 [Suppl] 1: 317–318
Kety SS (1951) The theory and application of the exchange of inert gas at the lung and tissues. Pharmacol Rev 3: 1–41
Kozlowski MR, Marshall JF (1980) Plasticity of (14C) 2-deoxy-d-glucose incorporation into neostriatum and related structures in response to dopamine neuron damage and apomorphine replacement. Brain Res 197: 167–183
Kozlowski MR, Marshall JF (1983) Recovery of function and basal ganglia (14C) 2-deoxyglucose uptake after nigrostriatal injury. Brain Res 259: 237–248
McCulloch J, Savaki HE, Sokoloff L (1980) Influence of dopaminergic systems on the lateral habenular nucleus of the rat. Brain Res 194: 117–124
McCulloch J, Kelly PAT, Crome JJ (1981) GABAergic influence upon cerebral metabolism and perfusion. In: Cervos-Navarro J, Fritschka E (eds) Cerebral microcirculation and metabolism. Raven Press, New York, pp 189–193
Michenfelder JD, Sundt TM Jr (1971) Cerebral ATP and lactate levels in the squirrel monkey following occlusion of the middle cerebral artery. Stroke 2: 319–326
Norberg K, Siesjo BK (1975) Cerebral metabolism in hypoxic hypoxia I. Pattern of activation of glycolysis: a re-evaluation. Brain Res 86: 31–44
Pellegrino LJ, Cushman AJ (1968) A stereotaxic atlas of the rat brain. Appleton-Century-Crofts, New York
Pulsinelli WA, Duffy TE (1979) Local cerebral glucose metabolism during controlled hypoxia in rats. Science 204: 626–629
Rieke GL, Bowers DE Jr, Penn P (1981) Vascular supply pattern to rat caudatoputamen and globus pallidus: scanning electron microscopic study of vascular endocasts of stroke prone vessels. Stroke 12: 840–847
Robinson RG (1979) Differential behavioral and biochemical effects of right and left cerebral infarction in the rat. Science 205: 707–710
Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with (14C) iodoantipyrine. Am J Physiol 234: H 59–66
Savaki HE, Girault JA, Desban M, Glowinski J, Besson MJ (1984) Local cerebral metabolic effects induced by nigral stimulation following ventromedial thalamic lesions. I. Basal ganglia and related motor structures. Brain Res Bull 12: 609–616
Siesjo BK (1984) Cerebral circulation and metabolism. J Neurosurg 60: 883–908
Sokoloff L, Reivich M, Kennedy C, DesRosiers MH, Patlak CS, Petigrew KD, Sakurada O, Shinohara M (1977) (14C) deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rats. J Neurochem 28: 897–916
Strong AJ, Goodhardt MJ, Bronston NM, Symon L (1977) A comparison of the effects of ischemia on tissue flow, electrical activity and extracellular potassium ion concentration in the cerebral cortex of baboons. Biochem Soc Trans 5: 158–168
Symon L, Bronston NM, Chikovani O (1979) Ischemic brain edema following middle cerebral artery occlusion in baboons. Relationship between regional cerebral water content and blood flow at 1 to 2 hours. Stroke 10: 184–191
Tamura A, Graham DI, McCulloch J, Teasdale GW (1981 a) Focal cerebral ischemia in the rat: 1. Description of technique and early neurological consequences following middle cerebral artery occlusion. J Cereb Blood Flow Mctab 1: 53–60
Tamura A, Graham DI, McCulloch J, Teasedale GM (1981 b) Focal cerebral ischemia in the rat: 2. Regional cerebral blood flow determined by (14C) iodoantipyrine autoradiography following middle cerebral artery occlusion. J Cereb Blood Flow Metab 1: 61–69
Wechsler LR, Savaki KE, Sokoloff L (1979) Effects of d- and 1-amphetamine on local cerebral glucose utilization in the conscious rat. J Neurochem 32: 15–22
Welsh FA, O'Conner MJ, Langfitt TW (1977) Regions of cerebral ischemia locatedted by pyridine nucleotide fluorescence. Science 198: 951–953
Welsh FA, Greenberg JH, Jones SC, Ginsberg MD, Reivich M (1980) Correlation between glucose utilization and metabolite levels during focal ischemia in cat brain. Stroke 11: 79–84
Yonas H, Wolfson SK Jr, Dujovny M, Boenke M, Cook E (1981) Selective lentticulostriate occlusion in the primate. A highly focal cerebral ischemia model. Stroke 12: 567–572
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Shibuya, M., Arita, N. & Yamamoto, Y.L. Regional differences in local cerebral blood flow (LCBF) and glucose utilization (LCGU) in the basal ganglia after occlusion of the middle cerebral artery in rats. J. Neural Transmission 68, 271–287 (1987). https://doi.org/10.1007/BF02098503
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DOI: https://doi.org/10.1007/BF02098503