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A 23187-stimulated calcium uptake and GABA release by cerebrocortical synaptosomes: effects of high pressure

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Summary

Guinea pig cerebrocortical synaptosome preparations were used to study the effect of compression to 62 ATA on45Ca2+ uptake and [3H]GABA release using a calcium ionophore A23187, which bypasses the voltage-sensitive calcium channel. Pressure was found to exert a suppressive effect on the A 23187-induced release of [3H]GABA, while having no significant effect on A 23187-stimulated45Ca2+ uptake. On the other hand, both depolarization-induced45Ca2+ uptake and [3H]GABA release were inhibited by pressure exposure. These results suggest that pressure may suppress GABA release by affecting pre-synaptic events subsequent to calcium influx.

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Author notes

  1. S. C. Gilman MSC, USN

    Present address: Department of Physiology, Armed Forces Radiobiology Research Institute, 20889-5145, Bethesda, Maryland, USA

Authors and Affiliations

  1. Diving Medicine Department, Naval Medical Research Institute, Bethesda, Md, USA

    S. C. Gilman MSC, USN, J. S. Colton & Y. Grossman

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  1. S. C. Gilman MSC, USN
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  2. J. S. Colton
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  3. Y. Grossman
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Gilman, S.C., Colton, J.S. & Grossman, Y. A 23187-stimulated calcium uptake and GABA release by cerebrocortical synaptosomes: effects of high pressure. J. Neural Transmission 86, 1–9 (1991). https://doi.org/10.1007/BF01250371

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  • DOI: https://doi.org/10.1007/BF01250371

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