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Mutual interactions between HIV-1 and cytokines in adherent cells during acute infection

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Summary

The production of cytokines by HIV-infected cells from adherent tissues as well as their effects on HIV replication in the same cells were investigated. CD4-transfected HeLa-T4-6c epithelial cells, CD4-positive normal lung fibroblasts and CD4-negative RD rhabdomyosarcoma cells were infected with HIV-1. All cultures were permissive for virus replication, which was completed within 48–72 h by Hela-T4-6c and RD cells and 2–3 weeks in normal fibroblasts. During the course of HIV replication, a series of cytokines (particularly IL-6 and TNFα) was produced and released in parallel to the peak of virus growth, in amounts varying with the cell system studied. Treatment of cultures with recombinant cytokines given at concentrations in the range of those induced by HIV-1 indicated that IL-6 and TNFα caused an increase of: i) CD4 expression, ii) HIV absorption to uninfected cells, and iii) release of infectious virions by infected cells. The fact that HIV-1 absorption and spread can be mediated by HIV-induced cytokines may be relevant in the pathogenesis of the in vivo disease, as it may constitute a possible self-enhancing model of HIV infection also in the solid tissues.

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References

  1. Ameglio F, Tilocca F, Arca MV, Alemanno L, Dolei A (1993) Ferritin downregulation in HIV-infected cells. AIDS Res Hum Retroviruses 9: 795–798

    PubMed  Google Scholar 

  2. Ankel H, Turriziani O, Antonelli G (1991) Prostaglandin A inhibits replication of human immunodeficiency virus during acute infection. J Gen Virol 72: 2797–2800

    PubMed  Google Scholar 

  3. Barillari G, Buonaguro L, Fiorelli V, Hoffman J, Michaels F, Gallo RC, Ensoli B (1992) Effects of cytokines from activated immune cells on vascular cell growth and HIV-1 gene expression. J Immunol 149: 3727–3734

    PubMed  Google Scholar 

  4. Bhat S, Spitalnik SL, Gonzalez-Scarano F, Silberberg DH (1991) Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein. Proc Natl Acad Sci USA 88: 7131–7134

    PubMed  Google Scholar 

  5. Breen EC, Rezai AR, Nakajima K, Beall GN, Mitsuyasu RT, Hirano T, Kishimoto T, Martinez-Mada O (1990) Infection with HIV is associated with elevated IL-6 levels and production. J Immunol 144: 480–484

    PubMed  Google Scholar 

  6. Campbell IL (1991) Cytokines in viral diseases. Curr Opin Immunol 3: 486–491

    PubMed  Google Scholar 

  7. Cheng-Mayer C, Rutka JT, Rosenblum ML, McHugh T, Stites DP, Levy JA (1987) Human immunodeficiency virus can productively infect cultured human glial cells. Proc Natl Acad Sci USA 84: 3526–3530

    PubMed  Google Scholar 

  8. Chesebro B, Wehrly K (1988) Development of a sensitive quantitative focal assay for human immunodeficiency virus. J Virol 62: 3779–3788

    PubMed  Google Scholar 

  9. Clapham PR, Weber JN, Whitby D, McIntosh K, Dalgleish AG, Maddon PJ, Deen KC, Sweet RW, Weiss RA (1989) Soluble CD4 blocks the infectivity of diverse strains of HIV and SIV for T cells and monocytes but not for brain and muscle cells. Nature 337: 368–370

    PubMed  Google Scholar 

  10. Cordiali Fei P, Massa A, Prignano G, Pietravalle M, Alemanno L, Vitelli G, Palamara G, Giglio A, Gandolfo G, Gentili G, Caprilli F, Ameglio F (1992) Behavior of several progression markers during the HIV-Ab seroconversion period. Comparison with later stages. Biol Regulat Homeostat Agents 6: 57–64

    Google Scholar 

  11. Dianzani F, Antonelli G, Capobianchi MR, De Marco P (1988) Replication of human immunodeficiency virus: yield of infectious virus under single growth cycle conditions. Arch Virol 103: 127–131

    PubMed  Google Scholar 

  12. Dolei A, Serra C, Arca MV, Toniolo A (1992) Acute HIV-1 infection of CD4-positive human lung fibroblasts. AIDS 6: 232–234

    PubMed  Google Scholar 

  13. Emilie D, Peuchmaur M, Maillot MC, Crevon MC, Brousse N, Delfraissy JF, Dromont J, Galanaud P (1990) Production of interleukins in human immunodeficiency virus-1-replicating lymph nodes. J Clin Invest 86: 148–159

    PubMed  Google Scholar 

  14. Grassi F, Meneveri R, Gullberg M, Lopalco L, Rossi GB, Lanza P, De Santis C, Brattsand G, Buttò S, Ginelli E, Beretta A, Siccardi A (1991) Human immunodeficiency virus type 1 gp120 mimics a hidden monomorphic epitope borne to class 1 major histocompatibility complex heavy chains. J Exp Med 174: 53–62

    PubMed  Google Scholar 

  15. Grossmann RM, Kroeger J, Yourish D, Granelli-Piperno A, Murray DP, May LT, Kupper TS, Sengal PB, Gottlieb AB (1989) Interleukin-6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes. Proc Natl Acad Sci USA 86: 6367–6371

    PubMed  Google Scholar 

  16. Haubrich RH, Takeda A, Koff W, Smith G, Ennis F (1992) Studies of antibody-dependent enhancement of human immunodeficiency virus (HIV) type 1 infection mediated by Fc receptors using sera from recipients of a recombinant gp160 experimental HIV-1 vaccine. J Infect Dis 165: 545–548

    PubMed  Google Scholar 

  17. Kekow J, Wachsman W, McCutchan JA, Cronin M, Carson DA, Lotz M (1990) Transforming growth factor β and noncytopathic mechanisms of immunodeficiency in human immunodeficiency virus infection. Proc Natl Acad Sci USA 87: 8321–8325

    PubMed  Google Scholar 

  18. Lafeuilade A, Pozot-Martin I, Quilchini R (1991) Increased interleukin 6 production is associated with disease progression in HIV infection. AIDS 5: 1139–1151

    PubMed  Google Scholar 

  19. McKeating JA, Griffits PD, Weiss RA (1990) HIV susceptibility conferred to human fibroblasts by cytomegalo virus-induced Fc receptor. Nature 343: 659–661

    PubMed  Google Scholar 

  20. Mellert W, Kleinschmidt H, Schmidt J, Festl H, Emler S, Roth WK, Erfle V (1990) Infection of human fibroblast and osteoblast-like cells with HIV-1. AIDS 4: 527–535

    PubMed  Google Scholar 

  21. Merrill JE, Koyanagi Y, Zack J, Thomas L, Martin F, Chen ISY (1992) Induction of interleukin-1 and tumor necrosis factor α in brain cultures by human immunodeficiency virus type 1. J Virol 66: 2217–2225

    PubMed  Google Scholar 

  22. Mosca JD, Bednarik DP, Raj NBK, Rosen CA, Sodroski CA, Haseltine WA, Pitha P (1987) Herpes simplex virus type 1 can reactivate transcription of latent immuno-deficiency virus. Nature 325: 67–70

    PubMed  Google Scholar 

  23. Munis JR, Richman DD, Kornbluth RS (1990) Human immunodeficiency virus-1 infection of macrophages in vitro neither induces tumor necrosis factor (TNF)/cachectin gene expression nor alters TNF/cachectin induction by lipopolysaccharide. J Clin Invest 85: 591–596

    PubMed  Google Scholar 

  24. Nelson JA, Wiley CA, Reynolds-Kohler C, Reese CE, Margatetten W, Levy JA (1988) Human immunodeficiency virus detected in bowel epithelium from a patient with gastrointestinal symptoms. Lancet 1: 259–262

    PubMed  Google Scholar 

  25. Pfizenmaier K, Scheurich P, Schluter C, Kronke M (1987) Tumor necrosis factor enhances HLA-A,B,C and HLA-DR gene expression in human tumor cells. J Immunol 138: 975–980

    PubMed  Google Scholar 

  26. Plata F, Garcia-Pons F, Ryter A, Lebargy F, Goodenow MM, Dat MHQ, Autran B, Mayaud C (1990) HIV-1 infection of lung alveolar fibroblasts and macrophages in humans. AIDS Res Hum Retroviruses 6: 979–986

    PubMed  Google Scholar 

  27. Poli G, Fauci AS (1992) The effect of cytokines and pharmacologic agents on chronic HIV infection. AIDS Res Hum Retroviruses 8: 191–197

    PubMed  Google Scholar 

  28. Poli G, Kinter A, Justement JS, Kehrl JH, Bressler P, Stanley S, Fauci AS (1990) Tumor necrosis factor α functions in an autocrine manner in the induction of human immunodeficiency virus expression. Proc Natl Acad Sci USA 87: 782–785

    PubMed  Google Scholar 

  29. Rosenberg ZF, Fauci AS (1990) Immunopathogenic mechanisms of HIV infection: cytokine induction of HIV expression. Immunol Today 11: 176–180

    PubMed  Google Scholar 

  30. Scheglovitova O, Capobianchi MR, Antonelli G, Guamnu D, Dianzani F (1993) CD4-positive lymphoid cells rescue HIV-1 replication from abortively infected human primary endothelial cells. Arch Virol 132: 267–280

    PubMed  Google Scholar 

  31. Sodrowski JG, Rosen CA, Haseltine WA (1984) Transacting transcriptional activation of the long terminal repeat of human T lymphotropic viruses in infected cells. Science 225: 381–385

    PubMed  Google Scholar 

  32. Steffan AM, Lafon ME, Gendrault JL, Schweitzer C, Royer C, Jaeck D, Arnaud JP, Schmitt MP, Aubertin AM, Kirn A (1992) Primary cultures of endothelial cells from the human liver sinusoid are permissive for human immunodeficiency virus type 1. Proc Natl Acad Sci USA 89: 1582–1586

    PubMed  Google Scholar 

  33. Tateno M, Gonzalez-Scarano F, Levy JA (1989) Human immunodeficiency virus can infect CD4-negative fibroblastoid cells. Proc Natl Acad Sci USA 86: 4287–4290

    PubMed  Google Scholar 

  34. Tornatore C, Nath A, Amemya K, Major E (1991) Persistent human immunodeficiency virus type 1 infection in human fetal glial cells reactivated by T-cell factor(s) or by the cytokines tumor necrosis factor α and interleukin-1β. J Virol 65: 6094–6100

    PubMed  Google Scholar 

  35. Vitkovic L, Wood GP, Major EO, Fauci AS (1991) Human astrocytes stimulate HIV-1 expression in a chronically infected promonocyte clone via interleukin-6. AIDS Res Hum Retroviruses 7: 723–727

    PubMed  Google Scholar 

  36. Yamato K, El-Wajjaoiu Z, Simon K, Koeffler HP (1990) Modulation of interleukin-1β RNA in monocytic cells infected with human immunodeficiency virus-1. J Clin Invest 86: 1109–1114

    PubMed  Google Scholar 

  37. Yahi N, Baghdiguian S, Moreau H, Fantini J (1992) Galactosyl ceramide (or a closely related molecule) is the receptor for human immunodeficiency virus type 1 on human colon epithelial HT29 cells. J Virol 66: 4848–4854

    PubMed  Google Scholar 

  38. Wakerfield PE, James WD, Samlaska CP, Meltzer MS (1991) Tumor necrosis factor. J Am Acad Dermatol 24: 675–685

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Microbiology and Virology, University of Sassari, Sassari

    A. Dolei, C. Serra, M. V. Arca & F. Tilocca

  2. Institute S. Gallicano, Rome

    M. Pietravalle, L. Alemanno & F. Ameglio

  3. Institute of Medicine and Public Health, Pavia University, Varese, Italy

    A. Toniolo

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  1. A. Dolei
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  2. C. Serra
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  3. M. V. Arca
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  4. F. Tilocca
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  5. M. Pietravalle
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  6. L. Alemanno
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  7. A. Toniolo
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  8. F. Ameglio
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Dolei, A., Serra, C., Arca, M.V. et al. Mutual interactions between HIV-1 and cytokines in adherent cells during acute infection. Archives of Virology 134, 157–168 (1994). https://doi.org/10.1007/BF01379114

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  • DOI: https://doi.org/10.1007/BF01379114

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