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Pathogenicity and vaccine efficacy of a thymidine kinase-deficient mutant of feline herpesvirus type 1 in cats

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Summary

We constructed a recombinant feline herpesvirus type 1 (FHV-1) which was deleted in a defined region (450 bp) within the thymidine kinase (TK) gene (C7301dlTK) [Yokoyama et al. (1995) J Vet Med Sci 57: 709–714]. In this report, we carried out two experiments to assess the pathogenicity and vaccine efficacy of the recombinant C7301dlTK in cats. The first experiment showed that, following multiple inoculation of the recombinant C7301dlTK by intraocular, intranasal and oral routes, the virus was sufficiently attenuated in cats, although a high titer of the virus was recovered from target organs (eye, nose, and mouth). In the second experiment, two intramuscular vaccinations with the recombinant C7301dlTK protected cats to a significant degree against subsequent challenge with the parent FHV-1 strain C7301 at 4 weeks after the last vaccination. These results demonstrate that the recombinant C7301dlTK is effective as a live attenuated vaccine with a clear genetic marker.

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References

  1. Bittle JL, York CJ, Newberne JW, Martin M (1960) Serologic relationship of new feline cytopathogenic viruses. Am J Vet Res 21: 547–550

    Google Scholar 

  2. Bittle JL, Rubic WJ (1974) Studies of feline viral rhinotracheitis vaccine. Vet Med Small Anim Clin 69: 1503–1505

    PubMed  Google Scholar 

  3. Bittle JL, Rubic WJ (1975) Immunogenic and protective effects of the F-2 strain of feline viral rhinotracheitis virus. Am J Vet Res 36: 89–91

    PubMed  Google Scholar 

  4. Bittle JL, Rubic WJ (1975) A feline calicivirus vaccine combined with feline viral rhinotracheitis and feline panleukopenia vaccine. Feline Prac 5: 13–15

    Google Scholar 

  5. Cameron JM, McDougall I, Marsden HS, Preston VG, Ryan DM, Subak-Sharpe JH (1988) Ribonucleotide reductase encoded by herpes simplex virus is a determinant of the pathogenicity of the virus in mice and a valid antiviral target. J Gen Virol 69: 2607–2612

    PubMed  Google Scholar 

  6. Chou J, Kern ER, Whitley RJ, Roizman B (1990) Mapping of herpes simplex virus-1 neurovirulence to γ134.5, a gene nonessential for growth in culture. Science 250: 1262–1266

    PubMed  Google Scholar 

  7. Cole GE, Stacy-Phipps S, Nunberg JH (1990) Recombinant feline herpesviruses expressing feline leukemia virus envelope andgag proteins. J Virol 64: 4930–4938

    PubMed  Google Scholar 

  8. Crandell RA, Ganaway JR, Niemann WH, Maurer FD (1960) Comparative study of three isolates with the original feline viral rhinotracheitis virus. Am J Vet Res 21: 504–506

    PubMed  Google Scholar 

  9. Crandell RA, Maurer FD (1958) Isolation of a feline virus associated with intranuclear inclusion bodies. Proc Soc Exp Biol Med 97: 487–490

    PubMed  Google Scholar 

  10. Crandell RA, Fabricant CG, Nelson-Rees WA (1973) Development, characterization, and viral susceptibility of a feline (felis catrus) renal cell line (CRFK). In Vitro 9: 176–185

    PubMed  Google Scholar 

  11. Davis EV, Beckenbauer WH (1976) Studies on the safety and efficacy of an intranasal feline rhinotracheitis-calici virus vaccine. Vet Med Small Anim Clin 71: 1405–1410

    PubMed  Google Scholar 

  12. Edwards BG, Buell DJ, Acree WM (1977) Evaluation of a new feline rhinotracheitis virus vaccine. Vet Med Small Anim Clin 72: 205–209

    PubMed  Google Scholar 

  13. Edwards BG, Buell DJ, Acree WM, Payne JB (1977) Evaluation of feline rhinotracheitis/feline panleukopenia combination vaccines by consecutive virulent challenge. Feline Prac 7: 45–50

    Google Scholar 

  14. Efstathiou S, Kemp S, Darby G, Minson AC (1989) The role of herpes simplex virus type 1 thymidine kinase in pathogenesis. J Gen Virol 70: 869–879

    PubMed  Google Scholar 

  15. Field HJ, Wildy P (1978) The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus. J Hyg 81: 267–277

    Google Scholar 

  16. Grail A, Harbour DA (1990) Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1. Jpn J Vet Sci 52: 1007–1013

    Google Scholar 

  17. Herrmann SC, Gaskell RM, Ehlers B, Ludwig H (1984) Characterization of the feline herpesvirus genome and molecular epidemiology of isolates from natural outbreaks and latent infections. In: Wittmann A, Gaskell RM, Ehlers B, Rziha HJ (eds) Latent herpesvirus infection in vaterinary medicine. Martinus Nijihoff, Boston, pp 321–326

    Google Scholar 

  18. Hickman MA, Reubel GH, Hoffman DE, Morris JG, Rogers QR, Pedersen NC (1994) An epizootic of feline herpesvirus, type 1 in a large specific pathogen-free cat colony and attempts to eradicate the infection by identification and culling of carriers. Lab Anim 28: 320–329

    PubMed  Google Scholar 

  19. Horimoto T, Limcumpao JA, Xuan X, Ono M, Maeda K, Kawaguchi Y, Kai C, Takahashi E, Mikami T (1992) Heterogeneity of feline herpesvirus type 1 strains. Arch Virol 126: 283–292

    PubMed  Google Scholar 

  20. Johnson RH, Thomas RG (1966) Feline viral rhinotracheitis in Britain. Vet Rec 79: 188–190

    Google Scholar 

  21. Kimman TG, de Wind N, Oei-Lei N, Pol JMA, Berns AJM, Gielkens ALJ (1992) Contribution of single genes within the unique short region of Aujeszky's disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. J Gen Virol 73: 243–251

    PubMed  Google Scholar 

  22. Kimman TG, de Wind N, de Bruin T, de Visser Y, Voermans J (1994) Inactivation of glycoprotein gE and thymidine kinase or the US3-encoded protein kinase synergistically decreases in vivo replication of pseudorabies virus and the induction of protective immunity. Virology 205: 511–518

    PubMed  Google Scholar 

  23. Kit S, Kit M, Pirtle EC (1985) Attenuated properties of thymidine kinase-negative deletion mutant of pseudorabies virus. Am J Vet Res 46: 1359–1367

    PubMed  Google Scholar 

  24. Kit S, Qavi H, Gaines JD, Billingsley P, McConnell S (1985) Thymidine kinase-negative bovine herpesvirus type 1 mutant is stable and highly attenuated in calves. Arch Virol 86: 63–83

    PubMed  Google Scholar 

  25. Maeda K, Kawaguchi Y, Ono M, Tajima T, Mikami T (1995) Restriction endonuclease analysis of field isolates of feline herpesvirus type 1 and identification of the heterogeneous regions. J Clin Microbiol 33: 217–221

    PubMed  Google Scholar 

  26. Maeda K, Kawaguchi Y, Ono M, Tajima T, Mikami T (1995) Comparisons among feline herpesvirus type 1 isolates by immunoblot analysis. J Vet Med Sci 57: 147–150

    PubMed  Google Scholar 

  27. Mochizuki M, Konishi S, Ogata M (1977) Studies on cytopathogenic viruses from cats with respiratory infections. III. Isolation and certain properties of feline herpesviruses. Jpn J Vet Sci 39: 27–37

    Google Scholar 

  28. Mochizuki M, Konishi S, Ogata M (1977) Sero-diagnostic aspects of feline herpesvirus infection. Jpn J Vet Sci 39: 191–194

    Google Scholar 

  29. Nash AA, Leung K-N, Wildy P (1985) The T-cell-mediated immune response of mice to herpes simplex virus. In: Roizman B, Lopes C (eds) The herpesviruses, vol 4. Plenum Press, New York, pp 87–102

    Google Scholar 

  30. Nunberg JH, Wright DK, Cole GE, Petrovskis EA, Post LE, Compton T, Gilbert JH (1989) Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpesvirus gene homologs. J Virol 63: 3240–3249

    PubMed  Google Scholar 

  31. Orr CM, Gaskell CJ, Gaskell RM (1980) Interaction of an intranasal combined feline viral rhinotracheitis, feline calicivirus vaccine and the FVR carrier state. Vet Rec 23: 164–166

    Google Scholar 

  32. Povey RC (1979) A review of feline viral rhinotracheitis (feline herpesvirus I infection). Comp Immunol Microbiol Infect Dis 2: 373–387

    PubMed  Google Scholar 

  33. Povey RC, Wilson MR (1978) A comparison of inactivated feline viral rhinotracheitis and feline caliciviral disease vaccines with live-modified viral vaccines. Feline Prac 8: 35–42

    Google Scholar 

  34. Scott FW (1975) Evaluation of a feline viral rhinotracheitis vaccine. Feline Prac 5: 17–22

    Google Scholar 

  35. Scott FW (1977) Evaluation of a feline viral rhinotracheitis-feline calicivirus disease vaccine. Am J Vet Res 38: 229–234

    PubMed  Google Scholar 

  36. Slater E, York C (1976) Comparative studies on parental and intranasal inoculation of an attenuated feline herpesvirus. Dev Biol Standard 33: 410–416

    Google Scholar 

  37. Slater JD, Gibson JS, Field HJ (1993) Pathogenicity of a thymidine kinase-deficient mutant of equine herpesvirus 1 in mice and specific pathogen-free foals. J Gen Virol 74: 819–828

    PubMed  Google Scholar 

  38. Studdert MJ, Martin MC (1970) Virus diseases of the respiratory tract of cats: 1. Isolation of feline rhinotracheitis virus. Aust Vet J 46: 99–104

    PubMed  Google Scholar 

  39. Tan RJS (1970) Serological comparisons of feline respiratory viruses. Jpn J Med Sci Biol 23: 419–424

    PubMed  Google Scholar 

  40. Tenser RB, Ressel SJ, Fralish FA, Jones JC (1983) The role of pseudorabies virus thymidine kinase expression in trigeminal ganglion infection. J Gen Virol 64: 1369–1373

    PubMed  Google Scholar 

  41. Wardley RC, Berlinski PJ, Thomsen DR, Meyer AL, Post LE (1992) The use of feline herpesvirus and baculovirus as vaccine vectors for thegag andenv genes of feline leukaemia virus. J Gen Virol 73: 1811–1818

    PubMed  Google Scholar 

  42. Willemse MJ, Chalmers WSK, Cronenberg AM, Pfundt R, Strijdreen IGL, Sondermeijer PJA (1994) The gene downstream of the gC homologue in feline herpes virus type 1 is involved in the expression of virulence. J Gen Virol 75: 3107–3116

    PubMed  Google Scholar 

  43. Wilson JHG (1978) Intranasal vaccination against upper respiratory tract disease (URD) in the cat. II. Results of field studies under enzootic conditions in the Netherlands with a combined vaccine containing live attenuated calici- and herpesvirus. Comp Immunol Microbiol Infect Dis 1: 43–48

    PubMed  Google Scholar 

  44. Yokoyama N, Maeda K, Kawaguchi Y, Ono M, Tohya Y, Mikami T (1995) Construction of the recombinant feline herpesvirus type 1 deleted thymidine kinase gene. J Vet Med Sci 57: 709–714

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, Tokyo, Japan

    N. Yokoyama, K. Maeda, Y. Tohya, Y. Kawaguchi, Y. -S. Shin, M. Ono & T. Mikami

  2. Central Laboratory, Kyoritsu Shoji Co., Ibaraki, Japan

    S. Ishiguro & Y. Fujikawa

Authors
  1. N. Yokoyama
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  2. K. Maeda
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  3. Y. Tohya
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  4. Y. Kawaguchi
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  5. Y. -S. Shin
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  6. M. Ono
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  7. S. Ishiguro
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  8. Y. Fujikawa
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  9. T. Mikami
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Yokoyama, N., Maeda, K., Tohya, Y. et al. Pathogenicity and vaccine efficacy of a thymidine kinase-deficient mutant of feline herpesvirus type 1 in cats. Archives of Virology 141, 481–494 (1996). https://doi.org/10.1007/BF01718312

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  • DOI: https://doi.org/10.1007/BF01718312

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