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An intersecting anti-serum pool system for the immunofluorescent identification of respiratory viruses

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Summary

A method is described for the construction of five intersecting pools of guineapig antiserum to be used in the immunofluorescent identification of eight common respiratory viruses.

The component antisera are highly purified and produce no non-specific fluorescence at working titre before or after incorporation into pools. The antiserum pools are fully stable at 4°C for up to 2 weeks, and for at least 3 months when stored at −20° C.

Due to their intersecting nature, a direct “read-out” of the type of virus present is possible. The pool system also offers the advantage of built-in negative controls for each specimen examined.

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References

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Author notes

  1. R. G. Sommerville

    Present address: Virology Section, University Department of Microbiology, Belvidere Hospital, Glasgow, Scotland

Authors and Affiliations

  1. Virology Section, University Department of Microbiology, Belvidere Hospital, Glasgow, Scotland

    A. R. McLure & D. E. MacFarlane

Authors
  1. A. R. McLure
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  2. D. E. MacFarlane
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  3. R. G. Sommerville
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McLure, A.R., MacFarlane, D.E. & Sommerville, R.G. An intersecting anti-serum pool system for the immunofluorescent identification of respiratory viruses. Archiv f Virusforschung 37, 6–11 (1972). https://doi.org/10.1007/BF01241145

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  • DOI: https://doi.org/10.1007/BF01241145

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