Summary
Human rotavirus “KUN” strain was cultivated in a fetal rhesus monkey kidney cell line, MA 104 cells. Four types of virus particles in cells infected with KUN strain were clearly identified: nucleoid cores, single-shelled particles, double-shelled particles, and membrane band, “enveloped” particles. “Enveloped” particles were found only in the thin sections of infected cells. When first visible, the virus precursors appeared at the ribosome free membrane of rough endoplasmic reticulum (RER), increasing in size while simultaneously being coated with nucleocapsid, inner shell. Single-shelled particles were also synthesized within bundles of filaments of viroplasm in the cytoplasma. During subsequent virus maturation two types of “budding” processes were observed. Double-shelled particles arising at the RER membrane entered the cisternae of the RER through an exocytosis-like process. In contrast, the “enveloped” particles developed in the cisternae by being completely enclosed with RER membrane, and later during cytolysis released the single-shelled particles. These “enveloped” virus particles appeared to be the result of inefficient virus maturation at the last stage of outer capsid formation.
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References
Adams, W. R., Kralt, L.: Electron microscopic study of the intestinal epithelium of mice infected with an agent of epizootic diarrhea of infant mice. Am. J. Path.51, 39–69 (1967).
Altenburg, B. C., Graham, D. Y., Estes, M. K.: Ultrastructural study of rotavirus replication in cultured cells. J. gen. Virol.45, 75–85 (1980).
Banfield, W. G., Kasnic, G., Blackwell, J. H.: Further observations on the epizootic diarrhea of infant mice. An electron microscopic study. Virology36, 411–421 (1968).
Chasey, D.: Different particle types in tissue culture and intestinal epithelium infected with rotavirus. J. gen. Virol.37, 443–451 (1977).
Esparza, J., Gorziglia, M., Gil, F., Romer, H.: Multi-replication of human rotavirus in cultured cells: an electron microscopic study. J. gen. Virol.47, 461–472 (1980).
Estes, M. K., Graham, D. Y., Smith, E. M., Gerba, C. P.: Rotavirus stability and inactivation. J. gen. Virol.43, 403–409 (1979).
Fernelius, A. L., Ritchie, A. E., Classick, L. G., Norman, J. O., Mebus, C. A.: Cell culture adaption and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska. Arch. ges. Virusforsch.37, 114–130 (1972).
Holmes, I. H., Ruck, B. J., Bishop, R. F., Davidsan, G. P.: Infantile enteritis viruses: morphogenesis and morphology. J. Virol.16, 937–943 (1975).
Kimura, T., Murakami, T.: Tubular structures associated with acute nonbacterial gastroenteritis in young children. Inf. Imm.17, 157–160 (1977).
Kutsuzawa, T., Konno, T., Suzuki, S., Kapikian, A. Z., Ebina, T., Ishida, H.: Isolation human rotavirus subgroup 1 and 2 in cell cultures. J. Clin. Microbiology16, 727–730 (1982).
Lecatsas, G.: Electron microscopic study of the formation of bluetongue virus. Onderstepoort Journal of Veterinary Research35, 139–150 (1968).
Lacatsas, G.: Electron microscopic study of a reovirus type 1 in BHK21 cells. Onderstepoort Journal of Veterinary Research35, 151–158 (1968).
Matsumura, T., Stollar, V., Schlesinger, R. W.: Studies on the nature of dengue viruses. V. structure and development of dengue virus in Vero cells. Virology46, 344–355 (1971).
McNulty, M. S., Curran, W. L., McFerran, J. B.: The morphogenesis of a cytopathic bovine rotavirus in Madin-Darby bovine kidney cells. J. gen. Virol.33, 503–508 (1976).
McNulty, M. S.: Rotaviruses. J. gen. Virol.40, 1–18 (1978).
McNulty, M. S., Allan, G. M., Todd, D., McFerran, J. B.: Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Archs. Virol.61, 13–21 (1979).
Pearson, G. R., McNulty, M. S.: Ultrastructural changes in small intestinal epithelium of neonatal pigs infected with pig rotavirus. Arch. Virol.59, 127–136 (1979).
Petrie, B. L., Graham, D. Y., Estes, M. K.: Identification of rotavirus particle types. Intervirology16, 20–28 (1981).
Saif, L. J., Theil, K. W., Bohl, E. H.: Morphogenesis of porcine rotavirus in porcine kidney cell cultures and intestinal epithelial cells. J. gen. Virol.39, 205–217 (1978).
Stair, E. L., Mebus, C. A., Twiehaus, M. J., Underdahl, N. R.: Neonatal calf diarrhea. Electron microscopy of intestines infected with a reovirus-like agent. Vet. Pathol.10, 155–170 (1973).
Suzuki, H., Konno, T.: Reovirus-like particles in jejunal mucosa of a Japanese infant with acute infectious nonbacterial gastroenteritis. Tohoku J. exp. Med.115, 119–122 (1975).
Suzuki, H., Kutsuzawa, T., Konno, T., Ebina, T., Ishida, N.: Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells. Arch. Virol.70, 33–41 (1981).
Tralka, T. S., Costa, J., Robson, A.: Electron microscopic study of herpesvirus saimiri. Virology80, 158–165 (1977).
Welch, A. B., Thompson, T. L.: Physiochemical characterization of a neonatal calf diarrhea virus. Can. J. comp. Med.37, 287–294 (1973).
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Suzuki, H., Konno, T., Kitaoka, S. et al. Further observations on the morphogenesis of human rotavirus in MA 104 cells. Archives of Virology 79, 147–159 (1984). https://doi.org/10.1007/BF01310808
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DOI: https://doi.org/10.1007/BF01310808