Summary
Altered baby hamster kidney (BHK-R) cells which were subcultured in the continuous presence of HVJ (hemagglutinating virus of Japan — the Sendai strain of parainfluenza 1 virus) showed a resistance to the antiviral action of both type I and II interferons. No evidence for a direct inactivation of interferon molecules during incubation of BHK-R cells was obtained. After serial subculture of BHK-R cells in growth medium free of HVJ, surface membranes with the proper sialic acid residues were restored and the cells became susceptible to the interferon action. It is suggested that binding sites for interferons might be ranked above HVJ receptors in the “receptor gradient”.
This is a preview of subscription content, log in via an institution to check access.
References
Belardelli, F., Aliberti, A., Santurbano, B., Antonelli, G., D'Agnolo, G., Rossi, G. B.: Interferon-induced antiviral actions in Friend leukenia cells: Role of membrane gangliosides. Virology117, 391–400 (1982).
Besancon, F., Ankel, H.: Binding of interferon to gangliosides. Nature252, 478–480 (1974).
Branca, A. A., Baglioni, C.: Evidence that types I and II interferons have different receptors. Nature294, 768–770 (1981).
Friedman, R. M.: Interferon binding: The first step in establishment of antiviral activity. Science156, 1760–1761 (1967).
Ito, Y., Kimura, Y., Nagata, I., Kunii, A.: Production of interferon-like substance by mouse spleen cells through contact with BHK cells persistently infected with HVJ. Virology60, 73–84 (1974).
Kato, N., Nakashima, I., Ohta, M.: Interferon production in mice by the capsular polysaccharide ofKlebsiella pneumoniae. Infect. Immun.12, 1–6 (1975).
Kimura, Y., Aoki, H., Shimokata, K., Ito, Y., Takano, M., Maeno, K.: Characterization of altered BHK cells resistant to HVJ (Sendai virus) infection. J. gen. Virol.47, 193–197 (1980).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951).
Markwell, M. A. K., Svennerholm, L., Paulson, J. C.: Specific gangliosides function as host cell receptors for Sendai virus. Proc. Natl. Acad. Sci. U.S.A.78, 5406–5410 (1981).
Nagata, I., Kimura, Y., Ito, Y., Tanaka, T.: Temperature-sensitive phenomenon of viral maturation observed in BHK cells persistently infected with HVJ. Virology49, 453–461 (1972).
Schiffmann, D., Koschel, K.: Interferon induces an antiviral state in ganglioside-deficient transformed mouse fibroblasts. Med. Microbiol. Immunol.169, 281–289 (1981).
Sugita, K., Maru, M., Sato, K.: A sensitive plaque assay for Sendai virus in an established line of monkey kidney cells. Japan. J. Microbiol.18, 262–264 (1974).
Svennerholm, L.: Quantitative estimation of sialic acids. 3. An anion exchange resin method. Acta Chem. Scand.12, 547–554 (1958).
Vengris, V. E., Reynolds, F. H., jr., Hollenberg, M. D., Pitha, P. M.: Interferon action. Role of membrane gangliosides. Virology72, 486–493 (1976).
Warren, L.: The thiobarbituric acid assay of sialic acids. J. Biol. Chem.234, 1971–1975 (1959).
Yonehara, S., Ishii, A., Y-Takahashi, M.: Cell surface receptor-mediated internalization of interferon: Its relation to the antiviral activity of interferon. J. gen. Virol.64, 2409–2418 (1983).
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
Kimura, Y., Yokochi, T., Miyadai, T. et al. Reversible resistance to the antiviral action of interferon observed in altered BHK cells resistant to HVJ (Sendai virus) infection. Archives of Virology 82, 241–246 (1984). https://doi.org/10.1007/BF01311167
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311167