Summary
A three-dimensional network of structural filaments was visible with common electron microscopes in the cytoplasm ofEuglena gracilis green cells extracted with buffers containing the nonionic detergent Triton X-100. A similar filamentous web was detected at the periphery of critical point dried cells cleaved on grids by means of an adhesive tape. SDS-polyacrylamide gel electrophoresis of the detergent-resistent cytoskeleton showed that actin or actin-like proteins of molecular weight in the range of 43–45 K are not among the components having a structural role inEuglena. The significance of these findings was discussed in relation to the capability of the alga to change the cell shape.
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References
Bassi, M., Donini, A., 1984: Phallotoxin-visualization of F-actin in normal and chromium-poisonedEuglena cells. Cell Biol. int. Rep.8, 867–871.
Bouck, G. B., 1982: Flagella and the cell surface. In: The biology ofEuglena, Vol. III (Buetow, D. E., ed.), pp. 29–51. New York: Academic Press.
Bovee, E. C., 1982: Movement and locomotion ofEuglena. In: The biology ofEuglena, Vol. III (Buetow, D. E., ed.), pp. 143–164. New York: Academic Press.
Bre, M. H., El Ferjani, E., Lefort-Tran, M., 1981: Sequential protein-dependent steps in the cell cycle. Initiation and completion of division in vitamin B 12 replenishedEuglena gracilis. Protoplasma108, 301–318.
Cande, W. Z., Wolniak, S. M., 1978: Chromosome movement in lysed mitotic cells is inhibited by vanadate. J. Cell Biol.72, 552–567.
Capco, D. G., Penman, S., 1983: Mitotic architecture of the cell: the filament networks of the nucleus and cytoplasm. J. Cell Biol.96, 896–906.
Clayton, L., Pogson, C. I., Gull, K., 1983: Ultrastructural and biochemical characterisation of the cytoskeleton ofPhysarum polycephalum myxamoebae. Protoplasma118, 181–191.
Cox, G., Juniper, B. E., 1983: High-voltage electron microscopy of whole, critical-point dried plant cells. Fine cytoskeletal elements in the mossBryum tenuisetum. Protoplasma115, 70–80.
Fairbanks, G., Steck, T. L., Wallach, D. F. H., 1971: Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry10, 2606–2617.
Gallo, J. M., Schrevel, J., 1982: Euglenoid movements inDistigma proteus. I. Cortical rotational motion. Biol. Cell44, 139–148.
Hawes, C. R., 1981: Applications of high-voltage electron microscopy to botanical ultrastructure. Micron12, 227–257.
Hofmann, C., Bouck, B., 1976: Immunological and structural evidence for patterned intussusceptive surface growth in a unicellular organism. J. Cell Biol.69, 693–715.
Laemmli, U. K., 1970: Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature227, 680–685.
Lefort-Tran, M., Bre, M. H., Ranck, J. L., Pouphile, M., 1980:Euglena plasma membrane during normal and vitamin B 12 starvation growth. J. Cell Sci.41, 245–261.
Mesland, D. A. M., Spiele, H., Roos, E., 1981: Membraneassociated cytoskeleton and coated vesicles in cultured hepatocytes visualized by dry-cleaving. Exp. Cell Res.132, 169–184.
Metcalf III,T. N., Szabo, L. J., Schubert, K. R., Wang, J. L., 1980: Immunochemical identification of an actin-like protein from soybean seedlings. Nature285, 171–172.
Pahlic, M., 1985: Multiple forms of actin inPhysarum polycephalum. Eur. J. Cell Biol.36, 169–175.
Penman, S., Capco, D. G., Fey, E. G., Chatterjee, P., Reiter, T., Ermish, S., Wan, K., 1983: The three-dimensional structural networks of cytoplasm and nucleus: function in cells and tissue. In: Modern cell biology, Vol. II (McIntosh, J. R., ed.), pp. 385–415. New York: Alan R. Liss, Inc.
Pollard, T. D., 1976: The role of actin in the temperature dependent gelation and contraction of extracts ofAcanthamoeba. J. Cell Biol.68, 579–601.
Porter, K. R., Beckerle, M., McNiven, M., 1983: The cytoplasmic matrix. In: Modern cell biology, Vol. II (McIntosh, J. R., ed.), pp. 259–302. New York: Alan R. Liss, Inc.
Powell, A. J., Peace, G. W., Slabas, A. R., Lloyd, C. W., 1982: The detergent-resistant cytoskeleton of higher plant protoplasts contains nucleus-associated fibrillar bundles in addition to microtubules. J. Cell Sci.56, 319–335.
Rogalski, A. A., Bouck, G. B., 1980: Characterization and localization of a flagellar-specific membrane glycoprotein inEuglena. J. Cell Biol.86, 424–435.
Suzaki, T., Williamson, R. E., 1985: Euglenoid movement inEuglena fusca: evidence for sliding between pellicular strips. Protoplasma124, 137–146.
Traas, J. A., 1984: Visualization of the membrane bound cytoskeleton and coated pits of plant cells by means of dry cleaving. Protoplasma119, 212–218.
Vannini, G. L., Poli, F., 1983: Binucleation and abnormal chromosome distribution inEuglena gracilis cells treated with dimethyl sulfoxide. Protoplasma114, 62–66.
Wolken, J. J., 1961:Euglena. Inst. Microbiol. New Brunswick, New Jersey: Rutgers Univ. Press.
Wolosewick, J. J., Porter, K. R., 1979: Microtrabecular lattice of the cytoplasmic ground substance, artifact or reality? J. Cell Biol.82, 114–139.
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The study was supported by grants from Consiglio Nazionale delle Ricerche (CNR) and Ministero della Pubblica Istruzione of Italy.
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Poli, F., Pancaldi, S., Dall'Olio, G. et al. Cytoskeletal structures inEuglena gracilis after triton X-100 extraction and dry cleaving. Protoplasma 128, 218–223 (1985). https://doi.org/10.1007/BF01276344
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DOI: https://doi.org/10.1007/BF01276344