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Transformation of meristematic cells in the shoot apex of cultured pea shoots byAgrobacterium tumefaciens andA. rhizogenes

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Summary

Different tissues in cultured pea shoots were inoculated withAgrobacterium tumefaciens wild types C 58 and ACH 5 andA. rhizogenes wild type 9402. The C 58 and 9402 bacteria induced the formation of tumours and hairy roots respectively while the ACH 5 was inactive. The younger the tissue the more rapidly it responded to the active bacteria. The shoot apex was the most reactive organ and developed into a tumour, theA. rhizogenes tumours subsequently giving rise to transformed hairy roots. Histological examination showed that transformed cells (including those within the apical dome) initially became highly vacuolate before dividing rapidly to form a tumour. These changes were accompanied by cell division in surrounding tissues.

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Abbreviations

BAP:

6-benzylaminopurine

IBA:

indole-3-butyric acid

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Authors and Affiliations

  1. Department of Applied Genetics, AFRC Institute of Plant Science Research, John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    G. Hussey, R. D. Johnson & S. Warren

Authors
  1. G. Hussey
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  2. R. D. Johnson
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  3. S. Warren
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Hussey, G., Johnson, R.D. & Warren, S. Transformation of meristematic cells in the shoot apex of cultured pea shoots byAgrobacterium tumefaciens andA. rhizogenes . Protoplasma 148, 101–105 (1989). https://doi.org/10.1007/BF02079328

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  • DOI: https://doi.org/10.1007/BF02079328

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