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Pollen development in orchids

2. The cytokinetic apparatus in simultaneous cytokinesis

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Summary

Cytokinesis in microsporocytes of moth orchids is unusual in that it occurs simultaneously after meiosis, the cytoplasm does not infurrow in the division planes, and cell plates are deposited in association with centrifugal expansion of phragmoplasts. Microtubules radiating from the nuclear envelopes appear to be of fundamental importance in establishment of division planes. Primary interzonal spindles develop between sister nuclei and interaction of radial microtubules triggers development of secondary interzonal spindles between non-sister nuclei. From three to six or more phragmoplasts, depending upon the arrangement of nuclei in the coenocyte, develop from these postmeiotic arrays. The phragmoplasts consist of co-aligned microtubules and F-actin organized into bundles that are broad proximal to the mid-plane and taper distally. Ultrastructure of the phragmoplast/cell plate reveals that abundant ER is associated with vesicle aggregation and coalescence. Cell plates are deposited in association with phragmoplasts as they expand centrifugally to join the parental wall and/or fuse with one another in the interior of the cell.

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Abbreviations

CLSM:

confocal laser scanning microscope/microscopy

FITC:

flnorescein isothiocyanate

PPB:

preprophase band of microtubules

TEM:

transmission electron microscope/microscopy

References

  • Albert VA, Corriveau JL, Coleman AW (1989) In situ, fluorochromemediated visualization of nuclear and cytoplasmic DNA: a new cytological tool for orchid pollen research. Lindleyana 4: 192–214

    Google Scholar 

  • Bajer AS, Mole-Bajer J (1986) Reorganization of microtubules in endosperm cells and cell fragments of the higher plantHaemanthus in vivo. J Cell Biol 102: 263–281

    Google Scholar 

  • Brown RC, Lemmon BE (1988 a) Sporogenesis in bryophytes. Adv Bryol 3: 159–223

    Google Scholar 

  • — — (1988 b) Microtubules associated with simultaneous cytokinesis of coenocytic microsporocytes. Amer J Bot 75: 1848–1856

    Google Scholar 

  • — — (1988 c) Cytokinesis occurs at boundaries of domains delimited by nuclear-based microtubules in sporocytes ofConocephalum conicum (Bryophyta). Cell Motil Cytoskeleton 11: 139–146

    Google Scholar 

  • — — (1989) Minispindles and cytoplasmic domains in microsporogenesis in orchids. Protoplasma 148: 26–32

    Google Scholar 

  • — — (1991) Pollen development in orchids. 1. Cytoskeletal control of division plane in irregular patterns of meiotic cytokinesis. Protoplasma 163: 9–18

    Google Scholar 

  • Dunbar A (1973) Pollen ontogeny in some species of Campanulaceae. A study by electron microscopy. Bot Not 126: 277–315

    Google Scholar 

  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen inHelleborus foetidus L. II. Pollen grain development through the callose special wall stage. J Cell Sci 3: 175–186

    Google Scholar 

  • Farr CH (1916) Cytokinesis of the pollen-mother-cells of certain dicotyedons. Mem NY Bot Gard 6: 253–316, 3 plates

    Google Scholar 

  • — (1918) Cell division by furrowing inMagnolia. Amer J Bot 5: 379–395, 3 plates

    Google Scholar 

  • Gunning BES (1982) The cytokinetic apparatus: its development and spatial regulation. In: Lloyd C (ed) Cytoskeleton in plant growth and development. Academic Press, London, pp 229–292

    Google Scholar 

  • Hepler PK, Palevitz BA, Lancelle SA, McCauley MM, Lichtscheidl I (1990) Cortical endoplasmic reticulum in plants. J Cell Sci 96: 355–373

    Google Scholar 

  • Heslop-Harrison J (1971) Wall pattern formation in angiosperm microsporogenesis. Symp Soc Exp Biol 25: 277–300

    Google Scholar 

  • Kakimoto T, Shibaoko H (1987) Actin filaments and microtubules in the preprophase band and phragmoplast of tobacco cells. Protoplasma 140: 151–156

    Google Scholar 

  • Katsuta J, Hashiguchi Y, Shibaoka H (1990) The role of the cytoskeleton in positioning of the nucleus in premitotic tobacco BY-2 cells. J Cell Sci 95: 413–422

    Google Scholar 

  • Lloyd CW (1987) The plant Cytoskeleton: the impact of fluorescence microscopy. Annu Rev Plant Physiol 38: 119–139

    Google Scholar 

  • —, Traas JA (1988) The role of F-actin in determining the division plane in carrot suspension cells. Drug studies. Development 102: 211–221

    Google Scholar 

  • Mole-Bajer J, Bajer AS, Inoue S (1988) Three-dimensional localization and redistribution of F-actin in higher plant mitosis and cell plate formation. Cell Motil Cytoskeleton 10: 217–228

    Google Scholar 

  • Nakamura S, Miki-Hirosige H (1982) Coated vesicles and cell plate formation in the microspore mother cell. J Ultrastruct Res 80: 302–311

    Google Scholar 

  • Palevitz BA (1988) Cytochalasin-induced reorganization of actin inAllium root cells. Cell Motil Cytoskeleton 9: 283–298

    Google Scholar 

  • —, Hepler PK (1974 a) The control of the plane of division during stomatal differentiation inAllium. I. Spindle reorientation. Chromosoma 46: 297–326

    Google Scholar 

  • — — (1974 b) The control of the plane of division during stomatal differentiation inAllium. II. Drug studies. Chromosoma 46: 327–341

    Google Scholar 

  • Periasamy K, Swamy BGL (1959) Studies in the Annonaceae-I. Microsporogenesis inCananga odorata andMiliusa wightiana. Phytomorphology 9: 251–263

    Google Scholar 

  • Sampson FB (1969) Cytokinesis in pollen mother cells of angiosperms, with emphasis onLaurelia novae-zelandiae (Monimiaceae). Cytologia 34: 627–633

    Google Scholar 

  • Schnarf K (1929) Embryologie der Angiospermen. Berlin

  • Sheldon JM, Hawes C (1988) The actin cytoskeleton during male meiosis inLilium. Cell Biol Int Rep 12: 471–476

    Google Scholar 

  • Swamy BGL (1949) Embryological studies in the Orchidaceae. I. Gametophytes. Am Midl Nat 41: 184–201

    Google Scholar 

  • Traas JA, Burgain S, Dumas de Vaulx R (1989) The organization of the cytoskeleton during meiosis in eggplant (Solanum melongena L.): microtubules and F-actin are both necessary for coordinated meiotic division. J Cell Sci 92: 541–550

    Google Scholar 

  • Van Lammeren AAM, Bednara J, Willemse MTM (1989) Organization of the actin cytoskeleton during pollen development inGasteria verrucosa (Mill.) H Duval visualized with rhodaminephalloidin. Planta 178: 531–539

    Google Scholar 

  • Van Went J, Cresti M (1988) Cytokinesis in microspore mother cells ofImpatiens sultani. Sex Plant Reprod 1: 228–233

    Google Scholar 

  • Waterkeyn L (1962) Les parois microsporocytaires de nature callosique chezHelleborus etTradescantia. Cellule 62: 225–255, 3 plates

    Google Scholar 

  • Yeung EC (1987) Development of pollen and accessory structures in orchids. In: Arditti J (ed) Orchid biology: reviews and perspectives, vol 4. Cornell University Press, Ithaca, pp 193–226

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, The University of Southwestern Louisiana, 70504-2451, Lafayette, LA, USA

    R. C. Brown & B. E. Lemmon

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  1. R. C. Brown
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  2. B. E. Lemmon
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Brown, R.C., Lemmon, B.E. Pollen development in orchids. Protoplasma 165, 155–166 (1991). https://doi.org/10.1007/BF01322286

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  • DOI: https://doi.org/10.1007/BF01322286

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