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Renal handling of amino acids in 5/6-nephrectomized rats: Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone under amino acid load

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Summary

In anaesthetized adult female rats, the renal amino acid handling was measured six days after 5/6 nephrectomy (5/6NX). The distinct rise in blood urea nitrogen as well as the significant reduction in urine flow and GFR indicate an impairment of kidney function. In principle, in 5/6NX rats amino acid plasma concentrations were comparable to those of control animals with two intact kidneys, whereas the fractional excretions (FEAA) of most endogenous amino acids measured were significantly enhanced. After bolus injection of leucine or taurine (each 20 mg/100 g b.wt.) or glutamine (90 mg/ 100 g b.wt.), dissolved in 2m1 normal saline per 100 g b.wt., the FEAA of both the amino acids administered and the endogenous amino acids increased as a sign of overloaded amino acid reabsorption capacity. This effect was more pronounced in 5/6NX rats than in controls. As early as one hour after amino acid load, plasma concentrations and FEAA returned to baseline values of 5/6NX rats. A pretreatment with triiodothyronine (20,µg/100 g b.wt.) or dexamethasone (60 µg/100 g b.wt.), both given intraperitoneally once daily for 3 days, stimulated the renal amino acid transport capacity in 5/6NX rats: the increase in FEAA after amino acid load was significantly lower compared to non-pretreatred animals. This stimulation could be shown for the bolus amino acids and the endogenous amino acids and was more distinct in 5/6NX rats than in controls with two intact kidneys.

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References

  • Achtermeier S, Bräunlich H, Fleck C, Hohenegger M, Kretzschmar M, Sprott H (1993) Age dependent different consequences of bilateral nephrectomy (NX) in rats. Exp Toxicol Pathol 45: 149–153

    Google Scholar 

  • Anagnostou A, Miller M, Westenfelder C, Clemons G, Baranowski R (1988) Effects of triiodothyronine replacement on the anemia of chronic renal failure. Exp Hematol 16: 159–162

    Google Scholar 

  • Azimova S, Normatov K, Umarova G, Kalontarov A, Makhmudova A, Kashimova Z, Abdukarimov A (1986) Nature of thyroid hormone receptors. Intracellular functions of thyroid-binding prealbumin. Biochem 50: 1651–1659

    Google Scholar 

  • Baum M, Quigley R (1993) Glucocorticoids stimulate rabbit proximal convoluted tubule acidification. J Clin Invest 91: 110–114

    Google Scholar 

  • Betts PR, Green A (1977) Plasma and urine amino acid concentrations in children with chronic renal insufficiency. Nephron 18: 132–139

    Google Scholar 

  • Bräunlich H, Fleck C, Kersten L, Stein G, Laske V, Müller A, Keil E (1986) Renal effects of aluminium in uremic rats and in rats with intact kidney function. J Appl Toxicol 6: 55–59

    Google Scholar 

  • Capasso G, De Santo NG, Kinne R (1987) Thyroid hormones and renal transport: Cellular and biochemical aspects. Kidney Int 32: 443–451

    Google Scholar 

  • Ceriotti G, Spandrio L (1963) A spectrophotometric method for determination of urea. Clin Chim Acta 8: 295–299

    Google Scholar 

  • Chesney RW, Zelikovic I, Jones DP, Budreau A, Jolly K (1990) The renal transport of taurine and the regulation of renal sodium-chloride-dependent transporter activity. Pediatr Nephrol 4: 399–407

    Google Scholar 

  • Christensen HN (1990) Role of amino acid transport and countertransport in nutrition and metabolism. Physiol Rev 70: 43–71

    Google Scholar 

  • De Nayer P (1987) Thyroid hormone action at the cellular level. Hormone Res 26: 48–57

    Google Scholar 

  • Druml W, Kelly RA, May RC, Mitch WE (1988) Abnormal cation transport in uremia. Mechanism in adipocytes and skeletal muscle from uremic rats. J Clin Invest 81: 1197–1203

    Google Scholar 

  • Faraj AH, Morley AR (1992) Remnant kidney pathology after five-sixth nephrectomy in rat. 1. A biochemical and morphological study. APMIS 100: 1097–1105

    Google Scholar 

  • Fervenza FC, Harvey CM, Hendry BM, Ellroy JC (1989) Increased lysine transport capacity in erythrocytes from patients with chronic renal failure. Clin Sci 76: 419–422

    Google Scholar 

  • Fischerova H (1992) Renal excretion of amino acids in chronic renal failure and in chronically dialyzed patients. Cas Lek Cesk 131: 49–52

    Google Scholar 

  • Fleck C (1992) Renal transport of endogenous amino acids. II. Influence of treatment with triiodthyronine or dexamethasone in immature and adult rats. Renal Physiol Biochem 15: 266–276

    Google Scholar 

  • Fleck C, Bräunlich H (1984) Kidney function after unilateral nephrectomy. Exp Pathol 25: 3–18

    Google Scholar 

  • Fleck C, Bräunlich H (1986) Relation between renal and hepatic excretion of drugs: I Phenol red in comparison with p-aminohippurate and indocyanine green. Exp Pathol 29: 179–192

    Google Scholar 

  • Fleck C, Bräunlich H (1987) Relation between renal and hepatic excretion of drugs: 111. Comparison of various methods reducing the renal or hepatic excretory capacity of rats. Exp Pathol 31: 95–104

    Google Scholar 

  • Fleck C, Bräunlich H (1990) Age dependent differences in stimulation and compensation of renal and biliary transport processes. Correlation to the physicochemical properties of the excreted substances. Arzneim Forsch Drug Res 40: 1047–1052

    Google Scholar 

  • Fleck C, Bräunlich H (1995) Renal handling of drugs and amino acids after impairment of kidney or liver function — Influences of maturity and protective treatment. Pharmacol Ther 67: 53–77

    Google Scholar 

  • Fleck C, Börner A, Kretzschmar M, Machnik G, Sprott H, Zimmermann T, Keil E, Bräunlich H (1992) Liver function after bilateral nephrectomy. Liver 12: 319–325

    Google Scholar 

  • Fleck C, Aurich M, Schwertfeger M (1997) Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone in amino acid loaded rats. Amino Acids 12: 265–279

    Google Scholar 

  • Fröhlich J, Hoppe Seyler G, Schollmeyer P, Maier KP, Gerok W (1977) Possible sites of interaction of acute renal failure with amino acid utilization for gluconeogenesis in isolated perfused rat liver. Eur J Clin Invest 7: 261–268

    Google Scholar 

  • Greiber S, Mitch WE (1992) Mechanism for protein catabolism in uremia: metabolic acidosis and activation of proteolytic pathways. Miner Electrolyte Metab 18: 233–236

    Google Scholar 

  • Gretz N, Meisinger E, Waldherr R, Strauch M (1988) Acute renal failure after 5/6 nephrectomy: histological and functional changes. Contrib Nephrol 60: 56–63

    Google Scholar 

  • Guensey DL, Edelman IS (1983) Regulation of thermogenesis by thyroid hormones. In: Oppenheimer JH, Samuels HH (eds) Molecular basis of thyroid hormone action. Academic Press, New York, pp 293–294

    Google Scholar 

  • Gutmann M, Hoischen C, Krämer R (1993) Carrier mediated glutamate secretion by Corynebacterium glutamicum under biotin limitation. Biochim Biophys Acta 1112: 115–123

    Google Scholar 

  • Hatch M, Freel RW, Vaziri NC (1994) Intestinal excretion of oxalate in chronic renal failure. J Am Soc Nephrol 5: 1339–1343

    Google Scholar 

  • Haylor J, Chowdry J, Baillie H, Cope G, el Nahas AM (1996) Renal function and morphometry in the dwarf rat following a reduction in renal mass. Nephrol Dial Transplant 11: 643–650

    Google Scholar 

  • Ikeda T, Yuhara M, Gomi T, Ishimitsu T, Uehara Y (1989) Alteration of vascular thromboxane in rats with subtotal renal ablation. Prostaglandins Leukot Essent Fatty Acids 37: 157–161

    Google Scholar 

  • Kopple JD, Fukuda S (1980) Effects of amino acid infusion and renal failure on the uptake and release of amino acids by the dog kidney. Am J Clin Nutr 33: 1363–1372

    Google Scholar 

  • MacPherson NA, Moscarello MA, Goldberg DM (1991) Aminoaciduria is an earlier index of renal tubular damage than conventional renal disease markers in the gentamicin-rat model of acute renal failure. Clin Invest Med 14: 101–110

    Google Scholar 

  • Maroni BJ, Haesemeyer RW, Kutner MH, Mitch WE (1990) Kinetics of system A amino acid uptake by muscle: effects of insulin and acute uremia. Am J Physiol 258: F1304-F1310

    Google Scholar 

  • Michalk DV, Essich HJ, Bohles HJ, Scharer K (1983) Taurine metabolism in experimental renal failure. Kidney Int [Suppl] 15: S16-S21

    Google Scholar 

  • Mitch WE, May RC, Maroni BJ, Druml W (1989) Protein and amino acid metabolism in uremia: influence of metabolic acidosis. Kidney Int [Suppl] 27: S205-S207

    Google Scholar 

  • Nabokov A, Amann K, Wagner J, Gehlen F, Munter K, Ritz E (1996) Influence of specific and non-specific endothelin receptor antagonists on renal morphology in rats with surgical renal ablation. Nephrol Dial Transplant 11: 514–520

    Google Scholar 

  • Peterson LN, Carpenter B, Guttierrez GA, Fajardo C, Levine DZ (1987) Potassium depletion enhances renal compensatory hypertrophy in the nephrectomized rat. Miner Electrolyte Metab 13: 57–62

    Google Scholar 

  • Quan ZY, Walser M, Hill GS (1992) Adrenalectomy ameliorates ablative nephropathy in the rat idependently of corticosterone maintenance level. Kidney Int 41: 326–333

    Google Scholar 

  • Rafestin-Oblin ME, Lombes M, Lustenberger P, Blanchardic P, Michaud A, Claire M (1986) Affinity of corticosteroids for mineral corticoid and glucocorticoid receptors of the rabbit kidney: effect of steroid substitution. J Steroid Biochem 25: 527–534

    Google Scholar 

  • Rogers SA, Miller SB, Hammerman MR (1996) Altered EGF expression and thyroxine metabolism in kidney following acute ischemic injury in rat. Am J Physiol 270: F21-F30

    Google Scholar 

  • Roth M (1971) Fluorescence reaction for amino acids. Anal Chem 43: 880–882

    Google Scholar 

  • Roth M, Hampai A (1973) Column chromatography of amino acids with fluorescence detection. J Chromatogr 83: 353–356

    Google Scholar 

  • Schaefer RM, Teschner M, Weissinger F, Duelk MJ, Peter G, Heidland A (1989) Catabolic effects of ethanol in chronically uremic rats. Nephron 51: 67–72

    Google Scholar 

  • Silbernagl S (1983) Kinetics and localisation of tubular resorption of “acidic” amino acids. A microperfusion and free flow micropuncture study in rat kidney. Pflügers Arch Eur J Physiol 396: 218–224

    Google Scholar 

  • Silbernagl S (1992) Amino acids and oligopeptides. In: Seldin DW, Giebisch G (eds) The kidney, vol 2. Raven Press, New York, pp 2889–2920

    Google Scholar 

  • Sohtell M, Kalmark B, Ulfendahl H (1983) FITC-inulin as a kidney tubule marker in the rat. Acta Physiol Scand 119: 313–316

    Google Scholar 

  • Trachtman H, Lu P, Sturman JA (1993) Immunohistochemical localization of taurine in rat renal tissue: studies in experimental disease states. J Histochem Cytochem 41: 1209–1216

    Google Scholar 

  • Yamamoto K (1985) Steroid receptor regulated transcription of specific genes and gene networks. Ann Rev Genet 19: 209–252

    Google Scholar 

  • Yao SY, Mazyka WR, Elliot JF, Cheeseman CI, Young JD (1994) Poly (A)+ RNA from the mucosa of rat jejunum induces novel Na−-dependent and Na+-independentleucine transport activities in oocytes of Xenopus laevis. Molec Membrane Biol 11: 109–118

    Google Scholar 

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  1. Ch. Fleck

    Present address: Institut für Pharmakologie und Toxikologie, Klinikum der Friedrich-Schiller-Universität Jena, D-07740, Jena, Germany

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  1. Institut für Pharmakologie und Toxikologie, Klinikum der Friedrich-Schiller-Universität Jena, D-07740, Jena, Germany

    K. Gräfe & I. Kart

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Fleck, C., Gräfe, K. & Kart, I. Renal handling of amino acids in 5/6-nephrectomized rats: Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone under amino acid load. Amino Acids 16, 149–164 (1999). https://doi.org/10.1007/BF01321533

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