Abstract
We evaluated the activation and inhibition effects of phosphatidylcholine (PC) and sphingomyelin (SM) on lipoprotein lipase (LPL) for medium or long chain-triglycerides (TG) in monolayers at the air/water interface. Monolayers of medium chain-TG, being in an expanded state at a surface pressure of 15 mN/m, showed low susceptibility to LPL in the subphase. Adding 50 mole% of PC or SM into these monolayers reduced the partial molecular area of the TG and enhanced the LPL activity. Monolayers of long chain-TG, being in a condensed state, showed high susceptibility of LPL either with or without PC. SM added to the long chain-TG monolayers, however, inhibited the LPL action. We investigated the interaction between TG and phospholipid on the basis of the collapse pressure-measurements of mixed monolayers. For medium chain-TG monolayers, PC and SM gave similar collapse pressure-composition profiles. Contrary to this, SM gave a markedly higher collapse pressure of long chain-TG than PC: SM stabilized the monolayer state of long chain-TG. These results suggested that I) orientation of the acyl chains of TG molecule in a monolayer is crucial for the LPL activity, and that II) strong interaction between SM and long chain-TG retards the substrate-transfer from the mixed monolayer to the catalytic pocket of LPL.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Havel RJ, Kane JP, Kashyap ML (1973) J Clin Invest 52:32–38
Emmerich J, Beg OU, Peterson J, Previato L, Brunzell JD, Brewer HB, Jr, Santamarina-Fojo S (1992) J Biol Chem 267:4161–4165
Faustinella F, Smith LC, Semenkovich CF, Chan L (1991) J Biol Chem 266: 9481–9485
Dugi KA, Dichek HL, Talley GD, Brewer H, Jr, Santamarina-Fojo S (1992) J Biol Chem 267:25086–25091
Wong H, Davis RC, Thuren T, Goers JW, Nikazy J, Waite M, Schotz MC (1994) J Biol Chem 269:10319–10323
Van Tilbeurgh H, Egloff M-P, Martinez C, Rugani N, Verger R, Cambillau C (1993) Nature 362:814–820
Vainio P, Virtanen JA, Kinnunen PKJ, Voyta JC, Smith LC, Gotto AM, Jr, Sparrow JT, Pattus F, Verger R (1983) Biochemistry 22:2270–2275
Jackson RL, Ponce E, McLean LR (1986) Biochemistry 25:1166–1170
Jackson RL, Pattus F, de Haas GH (1980) Biochemistry 19:373–378
Vainio P, Virtanen JA, Kinnunen PKJ, Gotto AM, Jr, Sparrow JT, Pattus F, Bougis P, Verger R (1983) J Biol Chem 258:5477–5482
Demel RA, Jackson RL (1985) J Biol Chem 260:9589–9592
Demel RA, Dings PJ, Jackson RL (1984) Biochim Biophys Acta 793:399–407
Nakagaki M, Handa T (1976) Bull Chem Soc Jpn 49:880–885
Nakagaki M, Tomita K, Handa T (1985) Biochemistry 24:4619–4624
Handa T, Saito H, Miyajima K (1993) Biophys J 64:1760–1765
Nakagaki M, Funasaki N, Fujita K (1972) J Chem Soc Jpn 1972:243–248
McLean LR, Demel RA, Socorro L, Shinomiya M, Jackson RL (1986) Methods Enzymol 129:738–763
Hayashi R, Tajima S, Yamamoto A (1986) J Biochem 100:319–331
Small DM (1986) Handbook of Lipid Research vol. 4, Plenum Press, New York, pp 345–394
Rogalska E, Ransac S, Verger R (1993) J Biol Chem 268:792–794
Wong H, Davis RC, Nikazy J, Seebart KE, Schotz MC (1991) Proc Natl Acad Sci USA 88:11290–11294
Thunnissen MMGM, AB E, Kalk KH, Drenth J, Dijkstra BW, Kuipers OP, Dijkman R, de Haas GH, Verheij HM (1990) Nature 347:689–691
Verheij HM, Slotboom AJ, de Haas GH (1981) Rev Physiol Biochem Pharmacol 91:91–203
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arimoto, I., Fujita, M., Saito, H. et al. Activation and inhibition of lipoprotein lipase in mixed monolayers of medium or long chain-triglycerides and phospholipids. Colloid Polym Sci 275, 60–66 (1997). https://doi.org/10.1007/s003960050052
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003960050052