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Electrically induced fusion of different human cell types

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Summary

The fusion of human cells of the cerebrospinal fluid and of the peripheral blood is reported, as well as the fusion of these cells with tomato protoplasts. The cells were fused by applying short-time electric pulses after dielectrophoretic collection. The importance of this method for diagnosis and therapy is discussed and possible applications are mentioned.

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References

  1. Baehr R von (1985) Die medizinische Bedeutung induzierter monoklonaler Antikörper — Ursprung, Stand und Perspektiven. Z Klin Med 40:85–94

    Google Scholar 

  2. Dommasch D (1975) Monocytes and histiocytes in cell cultures of cerebrospinal fluid. J Neurol 209:103–114

    Google Scholar 

  3. Dommasch D, Grüninger W, Schultze B (1977) Autoradiographic demonstration of proliferating cells in cerebrospinal fluid. J Neurol 214:97–112

    Google Scholar 

  4. Dudits D, Rasko I, Hadlaczky Gy, Lima-De-Faria A (1976) Fusion of human cells with carrot protoplasts induced by polyethyleneglycol. Hereditas 82:121–124

    Google Scholar 

  5. Guseo A (1971) Der Untergang der Liquorzellen in vivo und in vitro. Ideggyòg Szele 24:471–479

    Google Scholar 

  6. Jacob HE, Siegemund F, Bauer E (1984) Fusion von pflanzlichen Protoplasten durch elektrischen Feldimpuls nach Dielektrophorese. Biol Zentralbl 103:77–82

    Google Scholar 

  7. Küppers G, Diederich KJ, Zimmermann U (1984) Fusion by stimulated atmospheric discharges: further support for the hypotheses of involvement of electrofusion in evolution. Z Naturforsch 39:973–980

    Google Scholar 

  8. Lamprecht I, Mischel M (1981) Dielektrophoretische Untersuchungen an biologischen Systemen. Naturwissenschaften 68:501–506

    Google Scholar 

  9. Lo MUS, Tsong TY, Conrad MK, Strittmatter MS, Hester LD, Snyder SH (1984) Monoclonal antibody production by receptor-mediated electrically induced cell fusion. Nature 310:792–794

    Google Scholar 

  10. Müller W (1967) Über die Methodik der Liquorzellkultur. Ber Dtsch Phys Med Ges Würzburg 74:15–18

    Google Scholar 

  11. Müller W (1970) Beobachtungen an lebenden Liquorzellen unter Gewebekulturbedingungen. Z Neurol 198:315–331

    Google Scholar 

  12. Müller W, Versteeg J, van der Kuip L (1972) Die Kombination von Liquorzellkultur und Immunofluorescenz als diagnostische Methode. Z Neurol 202:159–163

    Google Scholar 

  13. Neumann E, Rosenheck K (1972) Permeability changes induced by electric impulses in vesicular membranes. J Membr Biol 10:279–290

    Google Scholar 

  14. Scheurich P, Zimmermann U (1981) Giant human erythrocytes by electric field-induced cell to cell fusion. Naturwissenschaften 68:45

    Google Scholar 

  15. Schmidt RM (1968) Der Liquor cerebrospinalis. VEB Volk und Gesundheit, Berlin

    Google Scholar 

  16. Schreier H (1982) Liposomen — ein neuartiger Arzneistoffträger. I. Phospholipide: Herstellung und Charakterisierung von Liposomen. Pharm Unserer Zeit 11:97–102

    Google Scholar 

  17. Schreier H, Raeder-Schikorr M (1982) Liposomen — ein neuartiger Arzneistoffträger. II. Schicksal von Liposomen in vivo: Einsatz in der Therapie. Pharm Unserer Zeit 11:103–108

    Google Scholar 

  18. Siegemund F, Golze P (1982) Effects of some conditions for the isolation of viable protoplasts of tomato, Lycopersicon esculentum Mill. Biològia (Bratisl) 37:1095–1102

    Google Scholar 

  19. Siegemund F, Tschuch G (1984) Elektrisch induzierte Fusion von Lycopersicon-Protoplasten unterschiedlicher Herkunft. Arch Züchtungsforsch 14:163–168

    Google Scholar 

  20. Vienken J, Zimmermann U, Alonso A, Chapman D (1984) Orientation of sickle red blood cells in an alternating electric field. Naturwissenschaften 71:158–159

    Google Scholar 

  21. Wieczorek V (1969) Klinische und tierexperimentelle Liquorzelluntersuchungen zur differentialdiagnostischen Abgrenzung und Bedeutung der einzelnen Zellarten im Liquor cerebrospinalis für die Diagnostik bei Erkrankungen im Bereich des ZNS. Habilitation thesis, Jena

  22. Zimmermann U (1982) Electric field-mediated fusion and related electric phenomena. Biochim Biophys Acta 694:227–277

    Google Scholar 

  23. Zimmermann U, Pilwat G, Richter HP (1981) Electric-field-stimulated fusion: increased field stability of cells induced by pronase. Naturwissenschaften 68:577–578

    Google Scholar 

  24. Zimmermann U, Scheurich PS, Pilwat G, Benz R (1981) Zellen mit manipulierten Funktionen: Neue Perspektiven für Zellbiologie, Medizin und Technik. Angew Chem 93:332–351

    Google Scholar 

  25. Zimmermann U, Vienken J, Pilwat G (1984) Electrofusion of cells. In: Chayen J, Bitensky L (eds) Investigative microtechniques in medicine and biology, vol. 1. Dekker, New York Basel

    Google Scholar 

  26. Zimmermann U, Vienken J, Pilwat G, Arnold WM (1984) Electrofusion of cells: principles and potential for the future. In: Pethica B, Whelan J (eds) Cell fusion. Pitman, London

    Google Scholar 

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Authors and Affiliations

  1. Klinik und Poliklinik für Psychiatrie und Neurologie der Martin-Luther-Universität Halle-Wittenberg, Julius-Kühn-Str. 7, DDR-4020, Halle/S

    V. Neumann & B. Baeßler

  2. Wissenschaftsbereich Genetik der Sektion Biowissenschaften, Martin-Luther-Universität Halle-Wittenberg, German Democratic Republic

    F. Siegemund

Authors
  1. V. Neumann
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  2. F. Siegemund
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  3. B. Baeßler
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Dedicated to Professor H. Rennert on the occasion of his 65th birthday

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Neumann, V., Siegemund, F. & Baeßler, B. Electrically induced fusion of different human cell types. J Neurol 233, 153–156 (1986). https://doi.org/10.1007/BF00314422

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  • DOI: https://doi.org/10.1007/BF00314422

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