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Separation and determination of components of spiramycin in bulk powders and in pharmaceutical preparations by high-performance liquid chromatography

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Summary

Two chromatographic systems are described to separate and estimate the three major components of the macrolide antibiotic spiramycin. The first system uses a C 18 reversed-phase column with acetonitrile-water-diethyl-amine (50:49.95:0.05) eluent, the second uses a microparticulate silica gel column with a mobile phase composed of the lower layer of a mixture of dichloromethane and n-propanol (96–4) saturated with 5% diethylamine in water.

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References

  1. R. Corbaz, L. Ettlinger, E. Gaüman, W. Keller-Schierlein, F. Kradolfer, E. Kyburz, L. Heipp, V. Prelog, A. Wettstein andH. Zähner, Helv. Chim. Acta39, 304 (1956).

    Article  Google Scholar 

  2. S. Pinnert-Sindico, L. Ninet, J. Preud'home andC. Cosar, Antibiolt. Ann. 724 (1954).

  3. Pharmacopéc Française VIII, 1965, Paris.

  4. R. Paul andS. Tchelitcheff, Bull. Soc. Chim. Fr. 650 (1965).

  5. M. E. Kuehne andB. W. Benson, J. Amer. Chem. Soc.87, 4660 (1965).

    Article  Google Scholar 

  6. S. Ōmura, A. Nakagawa, M. Otani, T. Hata, H. Ogura andK. Furuhata, J. Amer. Chem. Soc.91, 3401 (1969).

    Article  Google Scholar 

  7. T. Ikekawa, J. Antibiolt., Ser. A.16, 56 (1963).

    Google Scholar 

  8. S. Bhuwapathanapun andP. Gray, J. Antibiot.30, 673 (1977).

    PubMed  Google Scholar 

  9. K. Tsuji andJ. Goetz, J. Chromatogr.147, 359 (1978).

    Article  PubMed  Google Scholar 

  10. S. Ōmura, Y. Suzuki, A. Nakagawa andT. Hata, J. Antibiot.26, 794 (1973).

    PubMed  Google Scholar 

  11. A. Wehrli, J. C. Hildebrand, A. P. Keller, R. Stampfli andR. W. Frei, J. Chromatogr.149, 199 (1978).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry and Drug Quality Control, State University of Ghent, Faculty of Pharmaceutical Sciences, De Pintelaan 135, B-9000, Ghent, (Belgium)

    G. A. Bens, W. Van den Bossche & P. De Moerloose

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  1. G. A. Bens
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  2. W. Van den Bossche
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  3. P. De Moerloose
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Bens, G.A., Van den Bossche, W. & De Moerloose, P. Separation and determination of components of spiramycin in bulk powders and in pharmaceutical preparations by high-performance liquid chromatography. Chromatographia 12, 294–298 (1979). https://doi.org/10.1007/BF02261832

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  • DOI: https://doi.org/10.1007/BF02261832

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