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A new valve for zone-electrophoretic sample treatment coupled on-line with high performance liquid chromatography

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Summary

The design of a new valve arrangement for zone-electrophoretic sample treatment (ZEST) coupled online with high performance liquid chromatography is described. Characteristics of this valve, such as the internal heat development as a function of the current, have been investigated. By using quinidine and desipramine as model compounds it is shown that charged compounds can be isolated from biological samples, in about 15 min, with high selectivity. The carry-over of proteins to the analytical column has been compared with the carry-over using a pre-column sample clean-up method. The detection limits of quinidine and hydroquinidine (50 ng/ml), using zone-electrophoretic sample treatment coupled with column liquid chromatography, are in the same range as with direct injections using pre-columns.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Free University, de Boelelaan 1083, 1081 HV, Amsterdam, (The Netherlands)

    A. J. J. Debets, K.-P. Hupe & U. A. Th. Brinkman

  2. Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, (The Netherlands)

    W. Th. Kok

Authors
  1. A. J. J. Debets
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  2. K.-P. Hupe
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  3. U. A. Th. Brinkman
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  4. W. Th. Kok
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Debets, A.J.J., Hupe, KP., Brinkman, U.A.T. et al. A new valve for zone-electrophoretic sample treatment coupled on-line with high performance liquid chromatography. Chromatographia 29, 217–222 (1990). https://doi.org/10.1007/BF02317907

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  • DOI: https://doi.org/10.1007/BF02317907

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