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Evaluation of solute hydrophobicity by reversed-phase high performance liquid chromatography using aqueous binary mobile phases

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Summary

The dependence of the capacity factor (k′) on the concentration of the organic modifier (D) in the aqueous binary mobile phase in reversed-phase high-performance liquid chromatography has been investigated to evaluate the hydrophobicity of the solute molecule. The r-values, defined as the slope of log k′ vs. log(1/D) plots, were measured for various solutes and related to the non-polar surface area and the partition coefficients. The r-value was found to be a good indication of solute hydrophobicity. Detailed investigation of the results allowed to consider statistically the molecular posture of the solute adsorbed onto the stationary alkyl ligand.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, 606, Kyoto, Japan

    A. Kaibara, C. Hohda, N. Hirata, M. Hirose & T. Nakagawa

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  1. A. Kaibara
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  2. C. Hohda
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  3. N. Hirata
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  4. M. Hirose
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  5. T. Nakagawa
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Kaibara, A., Hohda, C., Hirata, N. et al. Evaluation of solute hydrophobicity by reversed-phase high performance liquid chromatography using aqueous binary mobile phases. Chromatographia 29, 275–288 (1990). https://doi.org/10.1007/BF02317918

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  • DOI: https://doi.org/10.1007/BF02317918

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