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Liquid chromatography of erythromycin A and related substances on poly(styrene-divinylbenzene)

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Summary

An isocratic liquid chromatography method for assay and purity control of erythromycin is presented. Erythromycin A is separated from all its potential impurities, except erythromycin D. The selectivity depends on the pore size of the poly(styrene-divinylbenzene) stationary phase. Wide pore PLRP-S 8 μm 1000 Å shows the best selectivity. The column is heated at 70°C. The mobile phase is acetonitrile-2-methyl-2-propanol-0.2 M potassium phosphate buffer pH 9.0-water (3:16.5:5:75.5). The flow rate is 2 ml/min. UV detection is performed at 215 nm. The total analysis time is about 30 min. The method was used to compare official standards.

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Authors and Affiliations

  1. Laboratorium voor Farmaceutische Chemie, Instituut voor Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Van Evenstraat 4, B-3000, Leuven, Belgium

    J. Paesen, E. Roets & J. Hoogmartens

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  1. J. Paesen
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  2. E. Roets
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  3. J. Hoogmartens
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Paesen, J., Roets, E. & Hoogmartens, J. Liquid chromatography of erythromycin A and related substances on poly(styrene-divinylbenzene). Chromatographia 32, 162–166 (1991). https://doi.org/10.1007/BF02325020

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  • DOI: https://doi.org/10.1007/BF02325020

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