Summary
A liquid chromatographic assay method was developed to separate 2′-deoxyuridine from its anomer and pentopyranosyl isomers. The influence of the pH of the mobile phase, the type of organic modifier and the column temperature on the chromatographic parameters was systematically investigated. Using a Hypersil C18 (5 μm) column at 10°C, the following mobile phase was found suitable: tetrahydrofuran −0.2 M potassium phosphate buffer pH 4.0-water (0.1∶5∶94.9, v/v).
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Van Schepdael, A., Van Aerschot, A., Herdewijn, P. et al. Liquid chromatographic separation of the anomers and isomers of 2′-deoxyuridine. Chromatographia 33, 571–574 (1992). https://doi.org/10.1007/BF02262250
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DOI: https://doi.org/10.1007/BF02262250