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Optimisation of fast protein separations on non-porous silica-based strong anion exchangers

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Summary

The adsorbed coating technology using various vinylpyrrolidone-vinylimidazole copolymer compositions was carried out on 1.7 μm non-porous monodisperse silica. It was shown that the retention properties and the loading capacity for bovine serum albumin (BSA) increases with the amount of vinylimidazole in the copolymer composition. The retention behavior of various proteins as a function of the salt composition in the eluent has been applied to find the optimal conditions for the synthesis of the anion-exchange stationary phase. The suitability of these supports for the fast separation of biological molecules is demonstrated. The best resolution and the highest speed in protein analysis was obtained by using an anion exchanger of low capacity.

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Authors and Affiliations

  1. Selbstemdikation, Boehringer Ingelheim GmbH, 55216, Ingelheim, Germany

    G. Jilge

  2. Laboratoire de Physico-Chimie des Biopolymères, CNRS, UMR 27, Université Paris Val de Marne, 2 rue H. Dunant, 94320, Thias, France

    B. Sébille, C. Vidal-Madjar & R. Lemque

  3. Insitut für Anorganische und Analytische Chemie, Johannes-Gutenberg-Universität, J. J. Becher Weg 24, 55099, Mainz, Germany

    K. K. Unger

Authors
  1. G. Jilge
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  2. B. Sébille
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  3. C. Vidal-Madjar
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  4. R. Lemque
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  5. K. K. Unger
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Jilge, G., Sébille, B., Vidal-Madjar, C. et al. Optimisation of fast protein separations on non-porous silica-based strong anion exchangers. Chromatographia 37, 603–607 (1993). https://doi.org/10.1007/BF02274109

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  • DOI: https://doi.org/10.1007/BF02274109

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