Abstract
Radiation hybrid (RH) maps are a useful tool for genome analysis, providing a direct method for localizing genes and anchoring physical maps and genomic sequence along chromosomes. The construction of a comprehensive RH map for the human genome1 has resulted in gene maps reflecting the location of more than 30,000 human genes2,3. Here we report the first comprehensive RH map of the mouse genome. The map contains 2,486 loci screened against an RH panel of 93 cell lines4. Most loci (93%) are simple sequence length polymorphisms (SSLPs) taken from the mouse genetic map, thereby providing direct integration between these two key maps. We performed RH mapping by a new and efficient approach in which we replaced traditional gel- or hybridization-based assays by a homogeneous 5´-nuclease assay5 involving a single common probe for all genetic markers. The map provides essentially complete connectivity and coverage across the genome, and good resolution for ordering loci, with 1 centiRay (cR) corresponding to an average of approximately 100 kb. The RH map, together with an accompanying World-Wide Web server, makes it possible for any investigator to rapidly localize sequences in the mouse genome. Together with the previously constructed genetic map6 and a YAC-based physical map reported in a companion paper7, the fundamental maps required for mouse genomics are now available.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hudson, T.J. et al. An STS-based map of the human genome. Science 270, 1945–1954 (1995).
Schuler, G.D. et al. A gene map of the human genome. Science 274, 540–546 (1996).
Deloukas, P.A. et al. A physical map of 30,000 human genes. Science 282, 744–746 (1998).
McCarthy, L.C. et al. A first-generation whole-genome radiation hybrid map spanning the mouse genome. Genome Res. 7, 1153–1161 (1997).
Livak, K.J., Marmaro, J. & Todd, J.A. Towards fully automated genome-wide polymorphism screening. Nature Genet. 9, 341–342 (1995).
Dietrich, W.F. et al. A comprehensive genetic map of the mouse genome. Nature 380, 149–152 (1996).
Nusbaum, C. et al. A YAC-based physical map of the mouse genome. Nature Genet. 22, 388–393 (1999).
Rhodes, M. et al. A high-resolution microsatellite map of the mouse genome. Genome Res. 8, 531–542 (1998).
Slonim, D., Kruglyak, L., Stein, L. & Lander, E.S. Building human genome maps with radiation hybrids. J. Comput. Biol. 4, 487–504 (1997).
Marra, M. et al. An encyclopedia of mouse genes. Nature Genet. 21, 191–194 (1999).
Acknowledgements
We thank G. Farino and V. Frazzoni for technical assistance. T.J.H. is a recipient of a Clinician-Scientist award from the Medical Research Council of Canada. This research was supported by the National Institutes of Health (HG01806).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Van Etten, W., Steen, R., Nguyen, H. et al. Radiation hybrid map of the mouse genome. Nat Genet 22, 384–387 (1999). https://doi.org/10.1038/11962
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/11962
This article is cited by
-
Female house mice initially shun infected males, but do not avoid mating with them
Behavioral Ecology and Sociobiology (2015)
-
Development of a deer mouse whole-genome radiation hybrid panel and comparative mapping of Mus chromosome 11 loci
Mammalian Genome (2006)
-
Genetic characterization of asymmetric somatic hybrids between Bupleurum scorzonerifolium Willd and Triticum aestivum L.: potential application to the study of the wheat genome
Planta (2006)
-
Initial sequencing and comparative analysis of the mouse genome
Nature (2002)
-
Two novel tumor suppressor gene loci on chromosome 6q and 15q in human osteosarcoma identified through comparative study of allelic imbalances in mouse and man
Oncogene (2002)