Abstract
The finding that the diversity (D) and joining (JH) but not the variable (VH) DNA segments of mouse immunoglobulin heavy-chain genes are joined in the DNA of some cloned cytolytic T cells, led to identification and sequencing of three different D DNA segments. Two segments identified on the embryo DNA carry on both the 5′ and 3′ sides two sets of characteristic sequences separated by a 12-base pair spacer, which have been implicated as recognition signals for a recombinase. The third segment, identified in a form joined with a JHDNA segment in a T cell, carries the recognition signal on the 5′ side. These results support the 12/23-base pair model for somatic generation of immunoglobulin V genes, and rule out the possibility that the cytolytic T cells use assembled VH, D and JH sequences to encode their antigen receptors.
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Kurosawa, Y., von Boehmer, H., Haas, W. et al. Identification of D segments of immunoglobulin heavy-chain genes and their rearrangement in T lymphocytes. Nature 290, 565–570 (1981). https://doi.org/10.1038/290565a0
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DOI: https://doi.org/10.1038/290565a0
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