Abstract
Fusions that bring lac gene expression under the control of transcriptional and translational signals within the Tn3 element have been used to identify and characterise a Tn3-encoded ‘transposase’ (Tnp) peptide of MW 100,000 essential f or transposition. The gene specifying this product is regulated by the Tn3 repressor protein and is part of a bidirectional genetic unit that includes the repressor gene.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Campbell, A. et al. in DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 15–22 (Cold Spring Harbor Laboratory, New York, 1977).
Heffron, F., Bedinger, P., Champoux, J. & Falkow, S. Proc. natn. Acad. Sci. U.S.A. 74, 702–706 (1977).
Heffron, F., So, M. & McCarthy, B. J. Proc. natn. Acad. Sci. U.S.A. 75, 6012–6016 (1978).
Kopecko, D. J. & Cohen, S. N. Proc. natn. Acad. Sci. U.S.A. 75, 1373–1377 (1975).
Cohen, S. N., Casadaban, M. J., Chou, J. & Tu, C.-P. D. Cold Spring Harb. Symp. quant. Biol. 43, 1247–1255 (1979).
Casadaban, M. J., Chou, J. & Cohen, S. N. (in preparation).
Chou, J., Casadaban, M. J., Lemaux, P. G. & Cohen, S. N. Proc. natn. Acad. Sci. U.S.A. 76, 4020–4024 (1979).
Gill, R., Heffron, F., Dougan, G. & Falkow, S. J. Bact. 136, 742–756 (1978).
Adler, H. I., Fisher, W. D., Cohen, A. & Hardegree, A. A. Proc. natn. Acad. Sci. U.S.A. 57, 321–329 (1967).
Sutcliffe, J. G. Proc. natn. Acad. Sci. U.S.A. 75, 3737–3741 (1978).
Casadaban, M. J., Chou, J. & Cohen, S. N. (in preparation).
Miller, J. H. in Experiments in Molecular Genetics,167–172 (Cold Spring Harbor Laboratory, New York, 1972).
Kretschmer, P. J., Chang, A. C. Y. & Cohen, S. N. J. Bact. 130, 888–899 (1977).
Kretschmer, P. J. & Cohen, S. N. J. Bact. 139, 515–519 (1979).
Sussenbach, J. S., Monfoort, C. H., Schiphof, R. & Stubberingh, E. E. Nucleic Acids Res. 3, 3193–3202 (1976).
Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).
Pribnow, D. Proc. natn. Acad. Sci. U.S.A. 72, 784–788 (1975).
Majors, J. Nature 256, 672–674 (1975).
Steege, D. A. Proc. natn. Acad. Sci. U.S.A. 74, 4163–4168 (1977).
Gilbert, W. & Maxam, A. Proc. natn. Acad. Sci. U.S.A. 70, 3581–3585 (1973).
Ptashne, M. et al. Science 194, 156–161 (1976).
Shapiro, J. Proc. natn. Acad. Sci. U.S.A. 76, 1933–1937 (1979).
Meagher, R. B., Tait, R. C., Betlack, M. & Boyer, H. W. Cell 10, 521–536 (1977).
O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Pederson, S. et al. Molec. gen. Genet. 144, 339–344 (1976).
Chang, A. C. Y. & Cohen, S. N. J. Bact. 134, 1141–1156 (1978).
Borck, K., Beggs, J., Brammar, W., Hopkins, A. & Murray, N. Molec. gen. Genet. 146, 199–207 (1976).
Dougan, G., Saul, M., Twigg, A., Gill, R. & Sherratt, D. J. Bact. 138, 48–54 (1979).
Timmis, K., Cabello, F. & Cohen, S. N. Molec. gen. Genet. 162, 121–137 (1978).
Cohen, S. N., Chang, A. C. Y. & Hsu, L. Proc. natn. Acad. Sci. U.S.A. 69, 2110–2114 (1972).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Neidhardt, F. C., Block, P. L. & Smith, D. F. J. Bact. 109, 736–747 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chou, J., Lemaux, P., Casadaban, M. et al. Transposition protein of Tn3: identification and characterisation of an essential represser-controlled gene product. Nature 282, 801–806 (1979). https://doi.org/10.1038/282801a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/282801a0
This article is cited by
-
Test systems to study transcriptional regulation and promoter activity in Bacillus megaterium
Applied Microbiology and Biotechnology (2005)
-
Transcriptional activation of flanking sequences by Tn1000 insertion
Molecular and General Genetics MGG (1994)
-
DNA binding domains in Tn3 transposase
Molecular and General Genetics MGG (1993)
-
ATP-dependent specific binding of Tn3 transposase to Tn3 inverted repeats
Nature (1985)
-
A New Chimeric Gene as a Marker for Plant Transformation: The Expression of Escherichia coli β-Galactosidase in Sunflower and Tobacco Cells
Nature Biotechnology (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.