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Radioimmunoassay of Angiotensin

Nature volume 217, pages 277–278 (1968)Cite this article

Abstract

HABER et al.1 showed that antibodies elicited in rabbits by copolymers of angiotensin with poly-L-lysine can be used for radioimmunoassay of angiotensin. The following are details and some results of the method we have developed. Comparing the binding affinity of three different rabbit antisera to Val5-angiotensin II amide (‘Hypertensin’, Ciba) and three analogues, we found that these antisera do not quantitatively behave identically. This fact has important consequences for the standardization of immunoassays of angiotensin in human plasma as long as human angiotensin is not available as a chemically pure substance. (Val5-angiotensin has the amino-acid sequence of bovine angiotensin, while Ileu5-angiotensin represents the structure of horse, hog and possibly human angiotensin.)

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Authors and Affiliations

  1. Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam

    H. J. G. HOLLEMANS, J. VAN DER MEER & J. L. TOUBER

  2. Department of Internal Medicine, Binnengasthuis, University of Amsterdam,

    H. J. G. HOLLEMANS, J. VAN DER MEER & J. L. TOUBER

Authors
  1. H. J. G. HOLLEMANS
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  2. J. VAN DER MEER
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  3. J. L. TOUBER
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HOLLEMANS, H., VAN DER MEER, J. & TOUBER, J. Radioimmunoassay of Angiotensin. Nature 217, 277–278 (1968). https://doi.org/10.1038/217277a0

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