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Cell type switching by DNA transposition in fission yeast

Nature volume 305, pages 682–688 (1983)Cite this article

Abstract

The mating-type genes of Schizosaccharomyces pombe have been isolated. Restriction and heteroduplex mapping have demonstrated that cell type switching occurs by copy transposition of a 1.1 kilobase sequence from one of two silent storage cassettes to an expression locus at mat1. The switching event is initiated by a long-lived double-strand DNA break at mat1. The properties of this cut are discussed in relation to the known pattern of cell type switching.

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References

  1. Egel, R. Molec. gen. Genet. 121, 277–284 (1973).

    Article  Google Scholar 

  2. Leupold, U. C. r. Lab. Carlsberg Ser. Physiol. 24, 381–480 (1950).

    Google Scholar 

  3. Egel, R. Nature 266, 172–174 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Miyata, H. & Miyata, M. J. gen. appl. Microbiol. 27, 365–371 (1981).

    Article  Google Scholar 

  5. Leupold, U. Cold Spring Harb. Symp. quant. Biol. 23, 161–170 (1958).

    Article  CAS  PubMed  Google Scholar 

  6. Egel, R. in DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. J., Shapiro, J. & Adhya, S.) 447–455 (Cold Spring Harbor, New York, 1977).

    Google Scholar 

  7. Egel, R. & Gutz, H. Curr. Genet. 3, 5–12 (1981).

    Article  CAS  PubMed  Google Scholar 

  8. Bresch, D., Muller, G. & Egel, R. Molec. gen. Genet. 102, 301–306 (1968).

    Article  CAS  PubMed  Google Scholar 

  9. Egel, R. Cold Spring Harb. Symp. quant. Biol. 45, 1003–1007 (1981).

    Article  PubMed  Google Scholar 

  10. Egel, R. Molec. gen. Genet. 161, 305–309 (1978).

    Article  Google Scholar 

  11. Egel, R. Molec. gen. Genet. (in the press).

  12. Oshima, Y. & Takano, I. Genetics 67, 327–335 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Hicks, J. B., Strathern, J. N. & Herskowitz, I. in DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. J., Shapiro, J. & Adhya, S.) 457–462 (Cold Spring Harbor, New York, 1977).

    Google Scholar 

  14. Hicks, J. B., Strathern, J. N. & Klar, A. J. S. Nature 282, 478–483 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Nasmyth, K. A. & Tatchell, K. Cell 19, 753–764 (1980).

    Article  CAS  PubMed  Google Scholar 

  16. Strathern, J. N., Spatola, E., McGill, C. & Hicks, J. B. Proc. natn. Acad. Sci. U.S.A. 77, 2839–2843 (1980).

    Article  ADS  CAS  Google Scholar 

  17. Beach, D. H., Nurse, P. M. & Egel, R. Nature 296, 682–683 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  18. Roberts, T. M. et al. Gene 12, 123–127 (1980).

    Article  CAS  PubMed  Google Scholar 

  19. Beach, D. H. & Nurse, P. M. Nature 290, 140–142 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Beach, D. H., Piper, M. & Nurse, P. M. Molec. gen. Genet. 187, 326–329 (1982).

    Article  CAS  PubMed  Google Scholar 

  21. Egel, R. Molec. gen. Genet. 161, 305–309 (1978).

    Article  Google Scholar 

  22. Strathern, J. N. et al. Cell 31, 183–192 (1982).

    Article  CAS  PubMed  Google Scholar 

  23. Gutz, H., Meade, J. H. & Walker, S. Genetics 80, Suppl. S38 (1975).

  24. Egel, R. Molec. gen. Genet. 148, 149–158 (1976).

    Article  CAS  PubMed  Google Scholar 

  25. Gutz, H. & Fecke, H. C. Hoppe-Seyler's Z. physiol. Chem. 360, 274 (1979).

    Google Scholar 

  26. Angehrn, P. & Gutz, H. Genetics 60, 158 (Abstr.), (1968).

    Google Scholar 

  27. Egel, R. & Egel-Mitani Expl Cell. Res. 88, 127–134 (1974).

    Article  CAS  Google Scholar 

  28. Nurse, P. M. Genetics as a Tool in Microbiology Vol. 31 (eds Glover, S. W. & Hopwood, D. A.) 291–315 (Cambridge University Press, 1981).

    Google Scholar 

  29. Gutz, H. & Doe, F. J. Genetics 74, 563–569 (1973).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Nurse, P. M., Thuriaux, P. & Nasmyth, K. A. Molec. gen. Genet. 146, 167–178 (1976).

    Article  CAS  PubMed  Google Scholar 

  31. Nasmyth, K. A. & Nurse, P. M. Molec. gen. Genet. 182, 119–124 (1981).

    Article  CAS  PubMed  Google Scholar 

  32. Hicks, J. B. & Herskowitz, I. Genetics 83, 245–258 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Strathern, J. N. & Herskowitz, I. Cell 17, 371–381 (1979).

    Article  CAS  PubMed  Google Scholar 

  34. Nasmyth, K. A. Nature 302, 670–676 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  35. Mao, J. et al. Nucleic Acids Res. 10, 487–500 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Russell, P. & Hall, B. D. Molec. cell. Biol. 2, 106–116 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Szostak, J. W., Orr-Weaver, T. L., Rothstein, R. J. & Stahl, F. W. Cell 33, 25–35 (1983).

    Article  CAS  PubMed  Google Scholar 

  38. Loenan, W. A. & Brammar, W. J. Gene 10, 249–259 (1980).

    Article  Google Scholar 

  39. Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  40. Chow, L. T. & Broker, T. R. Electron Microscopy in Biology Vol. 1 (ed. Griffith, J. D.) (Wiley, New York, 1981).

    Google Scholar 

  41. Southern, E. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  42. Mitchinson, J. M. Meth. cell. Physiol. 4, 131–165 (1970).

    Article  Google Scholar 

  43. Nurse, P. M. Nature 256, 547–551 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724, USA

    David H. Beach

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  1. David H. Beach
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Beach, D. Cell type switching by DNA transposition in fission yeast. Nature 305, 682–688 (1983). https://doi.org/10.1038/305682a0

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